ID A0A1T4KYW0_VIBCI Unreviewed; 829 AA.
AC A0A1T4KYW0;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Assimilatory nitrite reductase (NAD(P)H) large subunit {ECO:0000313|EMBL:SJZ47655.1};
GN ORFNames=SAMN02745782_00390 {ECO:0000313|EMBL:SJZ47655.1};
OS Vibrio cincinnatiensis DSM 19608.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=1123491 {ECO:0000313|EMBL:SJZ47655.1, ECO:0000313|Proteomes:UP000190834};
RN [1] {ECO:0000313|EMBL:SJZ47655.1, ECO:0000313|Proteomes:UP000190834}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19608 {ECO:0000313|EMBL:SJZ47655.1,
RC ECO:0000313|Proteomes:UP000190834};
RA Peterson S.W.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRNR:PIRNR037149};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC {ECO:0000256|ARBA:ARBA00005096}.
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
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DR EMBL; FUXB01000002; SJZ47655.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1T4KYW0; -.
DR STRING; 1123491.SAMN02745782_00390; -.
DR UniPathway; UPA00653; -.
DR Proteomes; UP000190834; Unassembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR012744; Nitri_red_NirB.
DR InterPro; IPR017121; Nitrite_Rdtase_lsu.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR InterPro; IPR041575; Rubredoxin_C.
DR NCBIfam; TIGR02374; nitri_red_nirB; 1.
DR PANTHER; PTHR43809; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR PANTHER; PTHR43809:SF1; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF01077; NIR_SIR; 1.
DR Pfam; PF03460; NIR_SIR_ferr; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR PIRSF; PIRSF037149; NirB; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 1.
DR SUPFAM; SSF55124; Nitrite/Sulfite reductase N-terminal domain-like; 1.
DR PROSITE; PS00365; NIR_SIR; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRNR:PIRNR037149};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|PIRNR:PIRNR037149}; Heme {ECO:0000256|ARBA:ARBA00022617};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063,
KW ECO:0000256|PIRNR:PIRNR037149};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 4..287
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 318..384
FT /note="NADH-rubredoxin oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF18267"
FT DOMAIN 414..462
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 551..611
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 623..748
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
SQ SEQUENCE 829 AA; 90984 MW; 2D7CB2E902387E13 CRC64;
MMEQIVIVGN GMVGHHLVAQ LVEKQAHLEK RITVIGEERF IAYDRVQLSS LFSGKSHDDL
MLSSEEWYRK QGINLILGCQ VTEINRAQKK LILDEDEVLG YDRLILATGS YPFVPPVPGR
ERDNIFVYRT LEDLSAIRLA CQGAKSGAVI GGGLLGLEAA NALRLLGLET HVVEFAPRLM
PVQLDDGGGA LLKSKIEHLG LKVHTSTVTE KIIDGESAKH RMVFKDAPAL EVDVIVFSAG
IRPQDTLARQ AGLEIGERGG IVISDQCLTS DKHIYAIGEC ALWQQKIFGL VAPGYAMARV
VADHLLGKSH AFSGADMSTK LKLLGVDVAS IGDAQMQSEG AKELVLQDTN QGTYKKLVVD
ESATRLLGAI LVGDSSDYDA LLQAYLNQTR LPEHPAHLLF DSSLLRGEMA DSSVICSCHN
VTKGALVAAI HAGATELAEL KAVTKAGTGC GGCSGMVKSV LDAELVTMGM EVNHHLCEHF
AYSRQELFHL CQVEQIKDFA TLLDKHGQGL GCDICKPTAA SVFASLWNEH IMQPDLSALQ
DSNDAFMANL QKDGSYSVVP RVAGGEITPD KLIVLGEVAK RYDLYTKITG GQRVDLFGAQ
MEQLPEIWQT LINAGFETGH AYGKSLRTVK SCVGSTWCRY GVDDSVGLAI ELEHRYKGLR
APHKLKFAVS GCTRECAEAQ SKDIGVIATE KGWNLYVCGN GGMRPRHGDL FAVDLSKDEL
VVLIDRVLMF YVRTADRLQR TSVWLDNLEG GVRYLKQVII EDSLGVCEQL EAQMQHVVDT
YQCEWKSTLE SEQKLRKFRP FINHQQGSMA LNYQRLREQR IPAEQGEIS
//
