ID A0A1T4QP19_9ACTN Unreviewed; 363 AA.
AC A0A1T4QP19;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Ribonuclease VapC {ECO:0000256|HAMAP-Rule:MF_00265};
DE Short=RNase VapC {ECO:0000256|HAMAP-Rule:MF_00265};
DE EC=3.1.-.- {ECO:0000256|HAMAP-Rule:MF_00265};
DE AltName: Full=Toxin VapC {ECO:0000256|HAMAP-Rule:MF_00265};
GN Name=vapC {ECO:0000256|HAMAP-Rule:MF_00265};
GN ORFNames=SAMN02745673_02346 {ECO:0000313|EMBL:SKA05444.1};
OS Marinactinospora thermotolerans DSM 45154.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Nocardiopsaceae; Marinactinospora.
OX NCBI_TaxID=1122192 {ECO:0000313|EMBL:SKA05444.1, ECO:0000313|Proteomes:UP000190637};
RN [1] {ECO:0000313|EMBL:SKA05444.1, ECO:0000313|Proteomes:UP000190637}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45154 {ECO:0000313|EMBL:SKA05444.1,
RC ECO:0000313|Proteomes:UP000190637};
RA Peterson S.W.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Toxic component of a toxin-antitoxin (TA) system. An RNase.
CC {ECO:0000256|HAMAP-Rule:MF_00265}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00265};
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000256|ARBA:ARBA00038093, ECO:0000256|HAMAP-Rule:MF_00265}.
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DR EMBL; FUWS01000005; SKA05444.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1T4QP19; -.
DR STRING; 1122192.SAMN02745673_02346; -.
DR OrthoDB; 32665at2; -.
DR Proteomes; UP000190637; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004521; F:RNA endonuclease activity; IEA:InterPro.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 2.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR039018; VapC20-like.
DR InterPro; IPR022907; VapC_family.
DR PANTHER; PTHR42188; 23S RRNA-SPECIFIC ENDONUCLEASE VAPC20; 1.
DR PANTHER; PTHR42188:SF1; 23S RRNA-SPECIFIC ENDONUCLEASE VAPC20; 1.
DR Pfam; PF01850; PIN; 2.
DR SUPFAM; SSF88723; PIN domain-like; 2.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00265};
KW Magnesium {ECO:0000256|HAMAP-Rule:MF_00265};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_00265};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_00265};
KW Reference proteome {ECO:0000313|Proteomes:UP000190637};
KW Toxin {ECO:0000256|HAMAP-Rule:MF_00265};
KW Toxin-antitoxin system {ECO:0000256|ARBA:ARBA00022649, ECO:0000256|HAMAP-
KW Rule:MF_00265}.
FT DOMAIN 2..116
FT /note="PIN"
FT /evidence="ECO:0000259|Pfam:PF01850"
FT DOMAIN 167..291
FT /note="PIN"
FT /evidence="ECO:0000259|Pfam:PF01850"
FT REGION 311..363
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 169
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00265"
FT BINDING 264
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00265"
SQ SEQUENCE 363 AA; 38748 MW; C7CB1753770D6F7E CRC64;
MIVADTGFLI ALLSEGDADH RACVELFERQ TEQVRVPASV VAGVSHALER LGAPQAEAAF
FDELNDRSRF VVVEHTRADY ARMADLVGRH PNSGFGTAEA GAVAVAERLG GPQIATVTPG
RYGGVRPEGV DYLLFAPPDQ SDALGPLRPR DPVPEDTEVA TRGDSAVLDT GVLVSLVTGD
RHTEASERWL ARSREELLVP DLIVGEAAFQ IRRLGAEGNR AEASFLRTLA EAPDVRVVNA
RPKDYGRMAQ IIREHADIKV GGCDASVLVA AERHRATSIA TVDSRDFQRL ADRAGASWRL
PLQADQAGRK AAVKDFPAGP GKALARGAGK KSRAGSGRAN PFRPAARLPD TAAGREGPRR
RGP
//
