ID A0A1T4U129_9GAMM Unreviewed; 612 AA.
AC A0A1T4U129;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE SubName: Full=Alcohol dehydrogenase (Cytochrome c) {ECO:0000313|EMBL:SKA46360.1};
GN ORFNames=SAMN02745132_00504 {ECO:0000313|EMBL:SKA46360.1};
OS Enterovibrio nigricans DSM 22720.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Enterovibrio.
OX NCBI_TaxID=1121868 {ECO:0000313|EMBL:SKA46360.1, ECO:0000313|Proteomes:UP000190162};
RN [1] {ECO:0000313|Proteomes:UP000190162}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22720 {ECO:0000313|Proteomes:UP000190162};
RA Varghese N., Submissions S.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR617512-3};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR617512-3};
CC -!- COFACTOR:
CC Name=pyrroloquinoline quinone; Xref=ChEBI:CHEBI:58442;
CC Evidence={ECO:0000256|PIRSR:PIRSR617512-2};
CC Note=Binds 1 PQQ group per subunit. {ECO:0000256|PIRSR:PIRSR617512-2};
CC -!- SIMILARITY: Belongs to the bacterial PQQ dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00008156}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FUXU01000004; SKA46360.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1T4U129; -.
DR Proteomes; UP000190162; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:InterPro.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0016614; F:oxidoreductase activity, acting on CH-OH group of donors; IEA:InterPro.
DR CDD; cd10277; PQQ_ADH_I; 1.
DR Gene3D; 2.140.10.10; Quinoprotein alcohol dehydrogenase-like superfamily; 1.
DR InterPro; IPR034119; ADHI.
DR InterPro; IPR018391; PQQ_beta_propeller_repeat.
DR InterPro; IPR017512; PQQ_MeOH/EtOH_DH.
DR InterPro; IPR002372; PQQ_repeat.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR001479; Quinoprotein_DH_CS.
DR NCBIfam; TIGR03075; PQQ_enz_alc_DH; 1.
DR PANTHER; PTHR32303; QUINOPROTEIN ALCOHOL DEHYDROGENASE (CYTOCHROME C); 1.
DR PANTHER; PTHR32303:SF20; QUINOPROTEIN ETHANOL DEHYDROGENASE; 1.
DR Pfam; PF13360; PQQ_2; 2.
DR SMART; SM00564; PQQ; 6.
DR SUPFAM; SSF50998; Quinoprotein alcohol dehydrogenase-like; 1.
DR PROSITE; PS00364; BACTERIAL_PQQ_2; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|PIRSR:PIRSR617512-3};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR617512-4};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR617512-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW PQQ {ECO:0000256|ARBA:ARBA00022891, ECO:0000256|PIRSR:PIRSR617512-2};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..612
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5012165251"
FT ACT_SITE 322
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-1"
FT BINDING 86
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT BINDING 136
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT BINDING 180
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT BINDING 198
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-3"
FT BINDING 280
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-3"
FT BINDING 322
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-3"
FT BINDING 419..420
FT /ligand="pyrroloquinoline quinone"
FT /ligand_id="ChEBI:CHEBI:58442"
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-2"
FT DISULFID 130..131
FT /evidence="ECO:0000256|PIRSR:PIRSR617512-4"
SQ SEQUENCE 612 AA; 67009 MW; E47345EEE2A8449B CRC64;
MTAMSFKRCG LFAALSLVLT APSMAAGVTD KDIMADQQTT EDVVTYGMGL RGQRFSPMDT
INKETVSEIR PVWGFSLGGE KQRGQESQPM IKDGVMYITG SYSRVYAIDA RTGDELWQYD
ARLPDGIMPC CDVINRGVAL YDDLVIFGTL DAKLVALDAK TGKVRWKKTV DNYQDGYSIT
AAPIVVKGNV ITGVSGGEFG IIGKVRAYDA KTGKLVWERP TVEGHMGYVW KDGKKIENGI
SGGAPGKTWP ADLWKSGGAA PWLGGTYDAD TDLLFFGTGN PAPWNSHMRP GDNLFSSSRL
AIDPDDGKIV WHFQTTPHDG WDFDGVNELI AFDYEQNGKT VKAAATADRN GFFYVLDRTS
GEFIRGFPFV NKISWAKGLD DKGRPLYIDS NRPGNPTDVA DGKKGQTVVA TPSFLGGKNW
MPMAYSPKSG LFYVPSNEWE MDIWNEPVSY KKGAAYLGAG FTIKSINDDY IGALRAYDPK
TGERVWEYKN YAPLWGGVMT TAGGLVFTGN PEGYLMAFDD KTGELLYKFN TGSGIVGTPV
TWEMDGEQYI SILSGWGERC HFGAETWPSG SRTSTKAVWS GRLNSPKTVR SLSGNTMPGE
SPAWCDQEVH HV
//
