ID   A0A1T4UTH1_9GAMM        Unreviewed;       340 AA.
AC   A0A1T4UTH1;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   27-MAR-2024, entry version 25.
DE   RecName: Full=L-lysine 2,3-aminomutase {ECO:0000256|ARBA:ARBA00022363};
DE   AltName: Full=EF-P post-translational modification enzyme B {ECO:0000256|ARBA:ARBA00030756};
GN   ORFNames=SAMN02745132_02483 {ECO:0000313|EMBL:SKA55960.1};
OS   Enterovibrio nigricans DSM 22720.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Enterovibrio.
OX   NCBI_TaxID=1121868 {ECO:0000313|EMBL:SKA55960.1, ECO:0000313|Proteomes:UP000190162};
RN   [1] {ECO:0000313|Proteomes:UP000190162}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 22720 {ECO:0000313|Proteomes:UP000190162};
RA   Varghese N., Submissions S.;
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-lysine = D-beta-lysine; Xref=Rhea:RHEA:44148,
CC         ChEBI:CHEBI:32551, ChEBI:CHEBI:84138;
CC         Evidence={ECO:0000256|ARBA:ARBA00001352};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000256|ARBA:ARBA00001933,
CC         ECO:0000256|PIRSR:PIRSR603739-50};
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. KamA family.
CC       {ECO:0000256|ARBA:ARBA00008703}.
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DR   EMBL; FUXU01000029; SKA55960.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1T4UTH1; -.
DR   OrthoDB; 9770937at2; -.
DR   Proteomes; UP000190162; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR022462; EpmB.
DR   InterPro; IPR003739; Lys_aminomutase/Glu_NH3_mut.
DR   InterPro; IPR007197; rSAM.
DR   NCBIfam; TIGR03821; EFP_modif_epmB; 1.
DR   NCBIfam; TIGR00238; KamA family radical SAM protein; 1.
DR   PANTHER; PTHR30538:SF1; L-LYSINE 2,3-AMINOMUTASE; 1.
DR   PANTHER; PTHR30538; LYSINE 2,3-AMINOMUTASE-RELATED; 1.
DR   Pfam; PF13353; Fer4_12; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   PIRSF; PIRSF004911; DUF160; 1.
DR   SFLD; SFLDF00314; L-lysine_2_3-aminomutase_(yjeK; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|PIRSR:PIRSR004911-1};
KW   Coiled coil {ECO:0000256|SAM:Coils}; Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRSR:PIRSR004911-
KW   1}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR004911-1};
KW   Pyridoxal phosphate {ECO:0000256|PIRSR:PIRSR603739-50};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          106..321
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
FT   COILED          226..253
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   BINDING         120
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004911-1"
FT   BINDING         124
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004911-1"
FT   BINDING         127
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004911-1"
FT   MOD_RES         332
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR603739-50"
SQ   SEQUENCE   340 AA;  38587 MW;  37378E79CB35B191 CRC64;
     MPDIITRNVP PVEQNWLKEM ANAVSDPQIL LQQLELEPTA WKSGFRARDL FAQRIPQSFI
     DRMEKGNPND PLLRQVMPVI DEFEDVPGYT TDPLEEQGNE QPGLLHKYKN RVLMILKGGC
     AINCRYCFRR HFPYEENKGG KSVWREAITY LQAHPEVDEV ILSGGDPLMA KDHELEWLIE
     AIESVPHVKR LRIHSRLPVV IPSRVTLALT NMLASTHLDV ILVTHINHTN EINEELRAAM
     TRLKQARVTL LNQGVLLKGV NDSVDTLKNL SNALFDAGIL PYYLHVLDKV QGAAHFLVDD
     EEAKRLMAGL IKEVSGYLVP RLTREIGGRD SKTPLDLHLE
//