ID A0A1T5EGS1_9FLAO Unreviewed; 756 AA.
AC A0A1T5EGS1;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Citrate hydro-lyase {ECO:0000256|ARBA:ARBA00029682};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN ORFNames=SAMN05660477_01400 {ECO:0000313|EMBL:SKB83079.1};
OS Soonwooa buanensis.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Chryseobacterium group; Soonwooa.
OX NCBI_TaxID=619805 {ECO:0000313|EMBL:SKB83079.1, ECO:0000313|Proteomes:UP000191112};
RN [1] {ECO:0000313|EMBL:SKB83079.1, ECO:0000313|Proteomes:UP000191112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22323 {ECO:0000313|EMBL:SKB83079.1,
RC ECO:0000313|Proteomes:UP000191112};
RA Peterson S.W.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; FUYZ01000003; SKB83079.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1T5EGS1; -.
DR STRING; 619805.SAMN05660477_01400; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000191112; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:InterPro.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.1060.10; Aconitase, Domain 2; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR015932; Aconitase_dom2.
DR InterPro; IPR006248; Aconitase_mito-like.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01340; aconitase_mito; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000191112}.
FT DOMAIN 35..478
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 559..686
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 756 AA; 81786 MW; F351E449364A8349 CRC64;
MIFDLDMIKK VYERYPERIA AARKVVGKPL TLAEKILYAH LWEGNATQAY ERGNSYVDFA
PDRVAMQDAT AQMALLQFMQ AGKAKVAVPS TAHADHLIQA RVGAEADLQE GINKNSEVFN
FLSSVCDKYG IGFWKPGAGI IHQVVLENYA FPGGMMIGTD SHTVNAGGLG MVAIGVGGAD
AVDVMAGMAW ELKMPKLIGV KLTGKMSGWT SAKDVILKVA GILTVKGGTG CIVEYFGEGA
ESLSGTGKGT ICNMGAEIGA TTSTFGYDDS MRRYLAATGR QEVVDAADKV AEHLTGDAEV
YANPEQYFDQ VIEINLSELT PHLNGPFTPD LATPVAEFRA KAEANGWPLE VEWALIGSCT
NSSYEDLSRA ASIVEDAVAK GVKPKAILGI NPGSEQVKFT AERDGFLDSF RKFENARIFT
NACGPCIGQW DREGAEKAEK NSIIHSFNRN FAKRADGNPN THAFVASPEM VAAVAISGRL
DFNPITDTLT NEAGEQVKLD EPKGSELPAK GFAVDDNGYQ APSEDGSNVQ VVVSPTSDRL
QLLEEFPAWD GLNITGAFVL IKAFGKCTTD HISMAGPWLK YRGHLDNISN NMLIGAVNAY
NMETNNVKNE LTGAYGEVPA VARAYKAAHV PSIVIGDENY GEGSSREHAA MEPRHLGVKA
VLVKSFARIH ETNLKKQGML GLTFADKADY DKVREDDVVN FLDLDKFAPG KQLTLEFVHN
DGTKDIVMAN HTYNDQQIDW FKAGSALNLI KRMEAN
//
