ID A0A1T5M4L4_9GAMM Unreviewed; 748 AA.
AC A0A1T5M4L4;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Malate dehydrogenase (Oxaloacetate-decarboxylating)(NADP+) {ECO:0000313|EMBL:SKC83083.1};
DE Flags: Fragment;
GN ORFNames=SAMN06296058_3636 {ECO:0000313|EMBL:SKC83083.1};
OS Pseudoxanthomonas indica.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Pseudoxanthomonas.
OX NCBI_TaxID=428993 {ECO:0000313|EMBL:SKC83083.1, ECO:0000313|Proteomes:UP000190341};
RN [1] {ECO:0000313|EMBL:SKC83083.1, ECO:0000313|Proteomes:UP000190341}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P15 {ECO:0000313|EMBL:SKC83083.1,
RC ECO:0000313|Proteomes:UP000190341};
RA Peterson S.W.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: In the N-terminal section; belongs to the malic enzymes
CC family. {ECO:0000256|ARBA:ARBA00007686}.
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DR EMBL; FUZV01000003; SKC83083.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1T5M4L4; -.
DR STRING; 428993.SAMN06296058_3636; -.
DR OrthoDB; 9805787at2; -.
DR Proteomes; UP000190341; Unassembled WGS sequence.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10950; -; 1.
DR Gene3D; 3.40.50.10750; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR012188; ME_PTA.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR042113; P_AcTrfase_dom1.
DR InterPro; IPR042112; P_AcTrfase_dom2.
DR InterPro; IPR002505; PTA_PTB.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR Pfam; PF01515; PTA_PTB; 1.
DR PIRSF; PIRSF036684; ME_PTA; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036684-2};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW NADP {ECO:0000256|PIRSR:PIRSR036684-3};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000190341}.
FT DOMAIN 13..146
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 158..393
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
FT ACT_SITE 89
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-1"
FT BINDING 71..78
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 131
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 132
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-2"
FT BINDING 157
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT BINDING 281
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000256|PIRSR:PIRSR036684-3"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:SKC83083.1"
FT NON_TER 748
FT /evidence="ECO:0000313|EMBL:SKC83083.1"
SQ SEQUENCE 748 AA; 80867 MW; 42FFA2D369D3A80B CRC64;
KQAALEYHRL SPPGKIKVTP TKPMLTQRDL ALAYSPGVAY ACEAIVEDPK AASELTARGN
LVAVVTNGTA VLGLGNIGAL AGKPVMEGKG VLFQKFAGID VFDIEIDETD PDKLVDIIAS
LEPTFGGINL EDIKAPECFI VERKLRERMR IPVFHDDQHG TAIIVGAAVV NALEVVGKRI
EDVKLATSGA GAAGIACLDM LVALGLKPEN ILAFDREGVI YSGRAHLDPD KARYARETDK
RTLAEIVAGA DIFLGLSAGG ILKPEMVATM AAKPIILALA NPNPEILPAD AKAVRPDAII
GTGRSDYPNQ VNNALCFPYI FRGALDVGAT VINEAMKVAC VHAIAHLARR EASDLGTAYG
DDVPCFGPEY LIPRPFDPRL LVEVAAAVAQ AAMDSGVALR PIQDMRGYRE KLGQFVYRTS
LMMKPVYDRA KADKKRVVYA EGEEETVLRA VQTVIDEGLA HPILIGRPDV IAARIERLSL
RMRAGVDFEL TNQDDDPRFN DYWQHYHALT SRRGVTPAAA KNLMRSRPTL IAAVMVARGE
ADAMVSGIVG RFHKKLGYVR SVIPLEPGVQ STSAMTGVIN NQGAYFFLDT HVQDDPTAEQ
IAEATLQAAY RLKLFGIEPR VALLSHSNFG SHETPGALKM RKVRELLLKR MPKLNVDGEM
QGDTAWDEVL RNRIMPGSTL TGRANLFVLP NLDAANIAYN MVRVVTDGVA IGPILMGISK
PVHILTTSAT PRRVVNMTAI AAVDAQIR
//
