ID   A0A1U7M541_TISCR        Unreviewed;       817 AA.
AC   A0A1U7M541;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   27-MAR-2024, entry version 15.
DE   RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE            EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN   ORFNames=TICRE_15790 {ECO:0000313|EMBL:OLS02427.1};
OS   Tissierella creatinophila DSM 6911.
OC   Bacteria; Bacillota; Tissierellia; Tissierellales; Tissierellaceae;
OC   Tissierella.
OX   NCBI_TaxID=1123403 {ECO:0000313|EMBL:OLS02427.1, ECO:0000313|Proteomes:UP000186112};
RN   [1] {ECO:0000313|EMBL:OLS02427.1, ECO:0000313|Proteomes:UP000186112}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6911 {ECO:0000313|EMBL:OLS02427.1,
RC   ECO:0000313|Proteomes:UP000186112};
RA   Poehlein A., Daniel R.;
RT   "Genome sequence of Tissierella creatinophila DSM 6911.";
RL   Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC         N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC         homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC         COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC         Evidence={ECO:0000256|ARBA:ARBA00001279};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OLS02427.1}.
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DR   EMBL; LTDM01000028; OLS02427.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1U7M541; -.
DR   REBASE; 193717; M.Tcr6911ORF15790P.
DR   Proteomes; UP000186112; Unassembled WGS sequence.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR003356; DNA_methylase_A-5.
DR   InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR   InterPro; IPR029464; HSDR_N.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR   PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR   Pfam; PF13588; HSDR_N_2; 1.
DR   Pfam; PF02384; N6_Mtase; 1.
DR   PRINTS; PR00507; N12N6MTFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00092; N6_MTASE; 1.
PE   4: Predicted;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW   ECO:0000313|EMBL:OLS02427.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000186112};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OLS02427.1}.
FT   DOMAIN          52..117
FT                   /note="Type I restriction enzyme R protein N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF13588"
FT   DOMAIN          343..681
FT                   /note="DNA methylase adenine-specific"
FT                   /evidence="ECO:0000259|Pfam:PF02384"
SQ   SEQUENCE   817 AA;  95650 MW;  0AE92532D9D36B3D CRC64;
     MEITKKQKEI INAYIEQSKI KAVESIDFVN KKVKYSNEIL NARNIDVLSG DEEIVRAYII
     TKLANELGYP LNKIVIEYEY TAGRPHTITS RIDLIVKDKN DDAFLFIELK PPKQYETLDK
     DSTIEEQLFK VAGMEKNEGH NVKYLVLYTI NFDNLNNIKD TCMIIDNEKY PNFAQWESVR
     NYTDTLPDRY SKVIKKPYIK NSDNDLEKSF THEMLHRLQI DLHNVLWGGG GTDDNEIFSS
     LTNLILAKIQ DEDEKMNGDA YDFQALSFVN DEDEFESNDV LFERINELYK RALSNKLNIV
     DKNELKKSYV IDTKKFSLSK LKYTVQQLEK YSFVDGKNSL SGKDILGDFF EGIIRNGFKQ
     DKGQFFTHIN IVKFMLWGIQ ADRLAIDRIN KDKEIPYMID PSAGSGTFLI EYMKFITENI
     KYKYRSQINS SREVTDKMES EWFYPNHREN KWAREYIYGT ELNFNLGTAT KVNMILHGDG
     STNIFVKDGL LPFNEYVKEN APNALNKAFK DNLYKYETNS QFDLILTNPP FSVDLDKDTA
     KRVKKNFIFG NKKNSENLFI ERYYQLLREN GRMAIILPES IFDTNENRYI RLFLYKYFNI
     KAIVSLPQST FAPFTNTKTS IIFAQKKTSE QIELWDSKWN IYTNEWSKLK NRVLNIIDVH
     HSVKSKEKLP SIKSLSQLEE IHILAQMLKN NLTIEDSNDI FSFCEDSAES DVLEFKESLI
     KKYEEDLISL CKVDNDTKAE FGFVNTWWVF SNVANELSYN IFMAEADEIG YKRTIRGERK
     SLNNLFRSDS NDKLIIDDGN KETILDYMRD IEWDEER
//