ID A0A1U7NFS9_9FIRM Unreviewed; 864 AA.
AC A0A1U7NFS9;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=BO222_06620 {ECO:0000313|EMBL:OLU39438.1};
OS Ileibacterium valens.
OC Bacteria; Bacillota; Erysipelotrichia; Erysipelotrichales;
OC Erysipelotrichaceae; Ileibacterium.
OX NCBI_TaxID=1862668 {ECO:0000313|EMBL:OLU39438.1, ECO:0000313|Proteomes:UP000186341};
RN [1] {ECO:0000313|EMBL:OLU39438.1, ECO:0000313|Proteomes:UP000186341}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NYU-BL-A3 {ECO:0000313|EMBL:OLU39438.1,
RC ECO:0000313|Proteomes:UP000186341};
RA Cox L.M., Sohn J., Tyrrell K.L., Citron D.M., Lawson P.A., Patel N.B.,
RA Iizumi T., Perez-Perez G.I., Goldstein E.J., Blaser M.J.;
RT "Description of two novel members of the family Erysipelotrichaceae:
RT Ileibacterium lipovorans gen. nov., sp. nov. and Dubosiella newyorkensis,
RT gen. nov., sp. nov.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|RuleBase:RU004460}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLU39438.1}.
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DR EMBL; MPJW01000135; OLU39438.1; -; Genomic_DNA.
DR RefSeq; WP_075819519.1; NZ_MPJW01000135.1.
DR AlphaFoldDB; A0A1U7NFS9; -.
DR GeneID; 82202871; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000186341; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000186341};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 2..261
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 622..828
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 864 AA; 98678 MW; 9568084B3AF44D8E CRC64;
MKKLLLVDGN SVLFRAYYST IYTNPMSTST GIPTNAVFGF ISMLNKAIST MQPDAILVAW
DAGKKTFRHD KFDDYKGTRK PLDEELIVQM PIVREFLDEN GIVRYEQEGY EADDIIGSMA
KAAEDMDVSI LTGDKDLLQL VNDHTKVLMM KKGVNQMAVM DEAAIDEQYG LKPIQIIDLK
GLMGDPSDNI PGVKHIGEKT ALLLLHKYGS IEETYAHLDE IKGKRHEYLE EGRDSALLSK
ELATIFTQMK LPFDIADLMY NPNEKNPNTF YQKYEMRSLL TSLPTQPEES KSASIIHTSE
CPFTAEDEII LMPVADQKPF MEQSLFGFML CENSPKKEAD KVYYLNKEDA LKDDSFKKLL
ENGRKITTWN VKESLHLFDR NGFALPNFQK DLHLAAFLLY SQATSLEALL EACEITLPVS
LKDLNKKSLG GFTEERAIPV FGKWISELPA LDQKLFALIE DENLHELYWD VEFPLARVLY
EMECQGISIS KSILLEIGKN YQARMDEIAQ RIYGYAGHTF NIGSPKQLGE VLFDELKLKS
STKKRSTAAE VLEKLRDAHP IIEDILEYRM YSKIVTTYVD GLKKYIIDDK IYTTFNQTMT
QTGRLSSSDP NLQNISIKSE EGREIRKAFV APENDYFISA DYSQVELRML AHMANEEFMI
EAFQEGIDIH NRTASIIFGV EPDQVTDAQR RTAKTVNFAI IYGQTEFGLS QELNISRVQA
RNFIRAYFQS YPNIHRFMDG LISGCEENGY VETLMHRRRY IPEIKDKNFM TREFGKRAAM
NAPIQGSAAD LIKIAMLRMD QAIKEKNLKS KMLLQIHDEL IFRVPEDELE LMQKLIPEVM
DSAMKLNVPL QASVNTGKTW YEAK
//
