ID A0A1U7R2I2_MESAU Unreviewed; 526 AA.
AC A0A1U7R2I2;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Hyaluronidase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
DE EC=3.2.1.35 {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
GN Name=Hyal5 {ECO:0000313|RefSeq:XP_005078156.1};
OS Mesocricetus auratus (Golden hamster).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea;
OC Cricetidae; Cricetinae; Mesocricetus.
OX NCBI_TaxID=10036 {ECO:0000313|Proteomes:UP000189706, ECO:0000313|RefSeq:XP_005078156.1};
RN [1] {ECO:0000313|RefSeq:XP_005078156.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00000251,
CC ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004609};
CC Lipid-anchor, GPI-anchor {ECO:0000256|ARBA:ARBA00004609}. Membrane
CC {ECO:0000256|ARBA:ARBA00004589}; Lipid-anchor, GPI-anchor
CC {ECO:0000256|ARBA:ARBA00004589}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 56 family.
CC {ECO:0000256|ARBA:ARBA00008871, ECO:0000256|PIRNR:PIRNR038193,
CC ECO:0000256|RuleBase:RU610713}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; XP_005078156.1; XM_005078099.1.
DR AlphaFoldDB; A0A1U7R2I2; -.
DR STRING; 10036.ENSMAUP00000007600; -.
DR GlyCosmos; A0A1U7R2I2; 1 site, No reported glycans.
DR GeneID; 101844137; -.
DR KEGG; maua:101844137; -.
DR CTD; 74468; -.
DR eggNOG; ENOG502R6HD; Eukaryota.
DR OrthoDB; 5344684at2759; -.
DR Proteomes; UP000189706; Unplaced.
DR GO; GO:0001669; C:acrosomal vesicle; IEA:Ensembl.
DR GO; GO:0009897; C:external side of plasma membrane; IEA:Ensembl.
DR GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IEA:InterPro.
DR GO; GO:0007341; P:penetration of zona pellucida; IEA:Ensembl.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR018155; Hyaluronidase.
DR InterPro; IPR001439; Hyaluronidase_PH20/Hyal5.
DR PANTHER; PTHR11769; HYALURONIDASE; 1.
DR PANTHER; PTHR11769:SF20; HYALURONIDASE PH-20; 1.
DR Pfam; PF01630; Glyco_hydro_56; 1.
DR PIRSF; PIRSF038193; Hyaluronidase; 1.
DR PIRSF; PIRSF500773; Hyaluronidase_PH20_Hyal5; 1.
DR PRINTS; PR00846; GLHYDRLASE56.
DR PRINTS; PR00848; SPERMPH20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR038193-3};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00022622};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR038193};
KW GPI-anchor {ECO:0000256|ARBA:ARBA00022622};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00022622};
KW Reference proteome {ECO:0000313|Proteomes:UP000189706}.
FT ACT_SITE 147
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-1"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-2"
FT DISULFID 60..351
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 223..237
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 376..387
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 381..435
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 437..443
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
SQ SEQUENCE 526 AA; 59740 MW; 338777BB2E4EBD82 CRC64;
MRVFCLKHSF SGSFLESNGA PHTVLVLLLL PCCLTADFRA PPLIPNVPFL WVWNAPTESC
LTKFNEPLDL SLFSLVGSPK KTATGQPITI FYTDRLGLYP YIDDSQTSVH GGIPQLGSLK
NHLAKSRNDI QHYMPIDNVG LAVIDWEDWR PTWSRNWKPK DIYRNKSIEL VKKQNIHLNL
TEATKKARKE FEQAARSFME QTLKLGKSLR PNHLWGFFLF PDCYNAQFHD ADYNGNCPDI
EKKKNDELSW IWKESTALYP SVYLRSRLKS SPQAALYVRN RVQEAIRVSK TKDPQNPVPI
FVYSRIVFAD LTYKFLHQDD LVNTIGETVA LGASGMVMWG TLGLVRSMKA CLNLHDYLEN
TLNPYLINVT LAAKMCSQTL CQDQGICSRK DWNSDNYLHL SPQNFQIQFL KSGKYVILGS
PTLDDLQYFS KNFRCSCYSN LNCKERDDLE SVSTVSVCAV EEACINSFVI ATPSALPVNW
KRPYFANSSQ SDIVSSATGG PCVPRRDVSG YLFVLSLYSQ HLKYLL
//
