ID A0A1U7VTJ6_NICSY Unreviewed; 1045 AA.
AC A0A1U7VTJ6;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Telomerase reverse transcriptase {ECO:0000256|ARBA:ARBA00016182, ECO:0000256|RuleBase:RU365061};
DE EC=2.7.7.49 {ECO:0000256|ARBA:ARBA00012493, ECO:0000256|RuleBase:RU365061};
DE AltName: Full=Telomerase catalytic subunit {ECO:0000256|RuleBase:RU365061};
GN Name=LOC104217220 {ECO:0000313|RefSeq:XP_009765714.1};
OS Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4096 {ECO:0000313|Proteomes:UP000189701, ECO:0000313|RefSeq:XP_009765714.1};
RN [1] {ECO:0000313|Proteomes:UP000189701}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA Peitsch M.C., Ivanov N.V.;
RT "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT tomentosiformis.";
RL Genome Biol. 14:R60.1-R60.17(2013).
RN [2] {ECO:0000313|RefSeq:XP_009765714.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_009765714.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Telomerase is a ribonucleoprotein enzyme essential for the
CC replication of chromosome termini in most eukaryotes. It elongates
CC telomeres. It is a reverse transcriptase that adds simple sequence
CC repeats to chromosome ends by copying a template sequence within the
CC RNA component of the enzyme. {ECO:0000256|RuleBase:RU365061}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.49;
CC Evidence={ECO:0000256|ARBA:ARBA00024557,
CC ECO:0000256|RuleBase:RU365061};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365061}.
CC Chromosome, telomere {ECO:0000256|RuleBase:RU365061}.
CC -!- SIMILARITY: Belongs to the reverse transcriptase family. Telomerase
CC subfamily. {ECO:0000256|ARBA:ARBA00008001,
CC ECO:0000256|RuleBase:RU365061}.
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DR RefSeq; XP_009765714.1; XM_009767412.1.
DR AlphaFoldDB; A0A1U7VTJ6; -.
DR GeneID; 104217220; -.
DR OrthoDB; 55913at2759; -.
DR Proteomes; UP000189701; Unplaced.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003721; F:telomerase RNA reverse transcriptase activity; IEA:InterPro.
DR CDD; cd01648; TERT; 1.
DR Gene3D; 1.10.132.70; -; 1.
DR Gene3D; 1.10.357.90; -; 1.
DR Gene3D; 3.30.70.2630; -; 1.
DR InterPro; IPR000477; RT_dom.
DR InterPro; IPR021891; Telomerase_RBD.
DR InterPro; IPR003545; Telomerase_RT.
DR InterPro; IPR049139; TERT_C.
DR PANTHER; PTHR12066; TELOMERASE REVERSE TRANSCRIPTASE; 1.
DR PANTHER; PTHR12066:SF0; TELOMERASE REVERSE TRANSCRIPTASE; 1.
DR Pfam; PF00078; RVT_1; 1.
DR Pfam; PF12009; Telomerase_RBD; 1.
DR Pfam; PF21399; TERT_C; 1.
DR PRINTS; PR01365; TELOMERASERT.
DR SMART; SM00975; Telomerase_RBD; 1.
DR PROSITE; PS50878; RT_POL; 1.
PE 3: Inferred from homology;
KW Chromosome {ECO:0000256|ARBA:ARBA00022454, ECO:0000256|RuleBase:RU365061};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365061};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365061};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU365061};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU365061};
KW Reference proteome {ECO:0000313|Proteomes:UP000189701};
KW RNA-directed DNA polymerase {ECO:0000256|RuleBase:RU365061,
KW ECO:0000313|RefSeq:XP_009765714.1};
KW Telomere {ECO:0000256|ARBA:ARBA00022895, ECO:0000256|RuleBase:RU365061};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365061}.
FT DOMAIN 475..851
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000259|PROSITE:PS50878"
SQ SEQUENCE 1045 AA; 119570 MW; 0C9A5557B1AE09A4 CRC64;
MRKKMCIRKN CAAEKLSTET TKSASGDVLP SREGQFYSIT HTSLCGKRTE TTKSASGDVL
PSREGQFYSI THTSLCGKRK RQFKWQRQRK RKQLKAQEKY SLIPCMNSNT KDNWPGDQNC
DSLISCMNSN TKDNWPGDQN CDVSSNSVLL LEKKNTWCSC CLVFQTIPEV KKEVQINRQT
IFYKLESFPS VFPSKHILNS LKPDSSGANR LFKEIFRSLG VNVTTQIASC THSSNCTFIR
STCLYHSIIK LLKTLIRKAH HCQHVRLLEK HCSSTPSLDQ DTKNIIATIL ERHETEASIL
AKGCAESGLA HNDIDSESCS TTPGSQPSLL EPTKCYCMKK QVVSFIWAVC RSIVPINLLG
TPSNWRVLTK NISKLVKLRR FEKFTLKQSM RKIKLSRYHL LSDQYSLLKH KLLEHWIFWF
FSSIVVPLVQ ANFYVTEAEH EKQDIFYYRK PTWENIISKF VTCLRDQGYQ ELNVASVKKI
IWNRSFGFSK VRLCPKGKGV RMLANLKASA KLPVNPPLQS KGLRKVGVGR NVKCYNYKSV
NEVLKDLHLV LKHIVVNEPE RLGSSVFDYN DAYKRLLPFL SLLKSGFSVK PGVFIIVSDV
EKAFDSVDQD KLLSVLDDLN LEEEYFLSKV VQVVCTKKSL RIPQNWTLVS KENAPDSANA
RSYLPTGSLH GILVKQQVQG RKVRKEQLQN DMKEHIRRNV LRLGSNFFLQ SVGIPQGSVL
SSMLCSLYLG HLEKSVIFPF LDKACEPAPG FPSEECFLDD TASGCAHLVM CKPSYLLLRF
IDDLLFISTS KEQASRFFSR LQRGFRAYNC DMNKKKFGMN FQINTIPDLR SDRLYVVEDG
TSFLRWSGLF INCSTLEIQA DYTRYLNFPL SSTLTVGWLN KPGRDLKVKL CGYLRPKCHP
IFYDSNINSA AVVRLNIYQA FLLCAMKFHC YIFDLSSICR FSTKFYAAAL GKSLRYMTKL
IKRRMFSFKT GSDFRPILEV GKGEIEWLGL TAYIRVLKRK QSRYKELLRV LDSKLMALGK
QESTLSVLQE ATDDKRSSIL WKIKY
//
