ID   A0A1U7WCT0_NICSY        Unreviewed;      1784 AA.
AC   A0A1U7WCT0;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE            EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
GN   Name=LOC104227270 {ECO:0000313|RefSeq:XP_009777792.1};
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096 {ECO:0000313|Proteomes:UP000189701, ECO:0000313|RefSeq:XP_009777792.1};
RN   [1] {ECO:0000313|Proteomes:UP000189701}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA   Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA   Peitsch M.C., Ivanov N.V.;
RT   "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT   tomentosiformis.";
RL   Genome Biol. 14:R60.1-R60.17(2013).
RN   [2] {ECO:0000313|RefSeq:XP_009777792.1}
RP   IDENTIFICATION.
RC   TISSUE=Leaf {ECO:0000313|RefSeq:XP_009777792.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC         arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC         an acidic residue or by a hydroxy-amino-acid residue, the
CC         phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC         Evidence={ECO:0000256|ARBA:ARBA00000451};
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DR   RefSeq; XP_009777792.1; XM_009779490.1.
DR   GeneID; 104227270; -.
DR   OrthoDB; 5479815at2759; -.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:InterPro.
DR   GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR   GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   InterPro; IPR005314; Peptidase_C50.
DR   InterPro; IPR030397; SEPARIN_core_dom.
DR   PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR   PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR   Pfam; PF03568; Peptidase_C50; 1.
DR   PROSITE; PS51700; SEPARIN; 1.
PE   4: Predicted;
KW   Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Reference proteome {ECO:0000313|Proteomes:UP000189701}.
FT   DOMAIN          1553..1647
FT                   /note="Peptidase C50"
FT                   /evidence="ECO:0000259|PROSITE:PS51700"
FT   REGION          882..902
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1784 AA;  201667 MW;  72E90EF28537D875 CRC64;
     MFSELVLLKD KPPTDLILGL YATGLLINES NIHSITQGNS RALSSDSKDV LQKLSSLLNF
     LKSYFEIDSK GSNTSQKRML YILSYFAVLK FLCQPLAEYV ISTRKEILSE TEAVSCNTYV
     EIIHDVFKQY INVFLHHSAA DSKQDHCDDT NKILLHVAVA TFTLSLRTKR DTKETVRFIK
     HLILSEWIQA NGLKYLFTSF YNITVVLYRN KQLIEAAKAL KLCCKASWNH VIRLCELFKH
     ESDKFQNDLS EDALIGFIDE ACGKTAFLLE VLNHSSDRKV QKVLIDSLKS WSAAEHLFKK
     LPSPISIVKQ FVKMELKVNN VDIEDGTTML YSLLSPFVES KQALGIILEQ ELLAYRELNA
     RNPRLCQEMC LKIIGLLLQK VYVSKDSYLQ RCRVLIVKGE VLRANGFENL KDCIQCLSEG
     IAIVESSLKQ KNCVANLACT NSASHLAAYA YCARALCTSE IEPNSKRLYE DIHAAARLWM
     SNHCHAPDQC RMSDIMLNML HQIVDLLSLK GYLELHPDIY EMMIQIFRKN IPLEKSVSLL
     WRYRRLSHAL CTSPVNDMFI KALSNHCGEL SESLKYWMKC MEVSQPQLIG FQQSFLTLAL
     SSKVSRSHQS FLHCDMTDDK VKLTAAELIH TVPLSSGAAF LSAYLYYDLS ERLILNGRLA
     EALAYAKEAH RLRSKLLQEN FFYQIEEQSE VYGLTAFQIY DPVAAKAWFP ENVSFDFDGS
     ILTPWTILQC YLESILQVGT VHEMLGNGTE AKTLLVWGEN ISSNQSLPLF IISFCCMLGK
     LYIKQHLWEL AEKELNTAKQ TLAENYDAIS CLKCRIILEV SIDLLIGDLW RLHHCDAVNS
     STVEFLYSVK EKYRSAFEKL NNFGWEDSVG WPLGASSQHT KHQATSFANG AKDPSGLKEL
     PSQMGKLDDA VEGRRTRKTK KESEHNLRMT RSRSYAMKKC ESSVDDKHSS GAENMSGMCF
     KLKCWHHLPL EVLQSGSLSN FIYLKWELVR RQLSLRLLTT MGKCIGLSGD IHGAQKLFLQ
     SVSLFSADSS CPKYSSLPLM SLVDQMGQDI WAVQLAVDHS LMLYHICCSI LNSYSCKATR
     KTSCKECHVF SCIKLSKVIS WLKLAFILSR EIPLLSQKIS RLLAAVYVLS TSDKSFSIPP
     SKAISESQWA SFFHQASIGT CLNQHFFSCP LKKQKAEHVV DYEGCCSLTQ QHLGSEEPNM
     FRLAPESVED LEDFVSRFFE SLPTSTVVCL SLLGRSVSSL LTELLHSPHP IQSWVLLSRM
     SSTSQPITVI LPAHSIVKEA SDDVAQFTSS FPFEVKDKHW HCPWLSSVVD DVAPVFRDIL
     ENNYLSSSVH LLEDTTESRS SWWKWRKQLD QRLAKFLRNL EDSWLGPWRY LLLGELSECE
     LLDSLVKKLY DHFRCKTGAD VHKSLLKVIL GAAKYAYEKE NHISQMVLNK GCHLHGGGNG
     NSKVFDKTST EVDNLFDSVY KSILDEAQEM VETESISRRP VILVLDLEVQ MLPWENLPLL
     RNQQVYRMPS ISSIRATLIK CCPYQQQVQM LKCYQGSPME QGVPSHSIPS IDPLDSYYLL
     NPSGDLSSTQ IEFESWFRDQ DFEGKCGTAP AVEELAEALK SHDLFIYFGH GSGAQYIPEH
     EVKKLESCAA TLLMGCSSGS LHLHGSYAPR GAALGYLLAG SPVIIANLWE VTDKDIDRFG
     KSMLDAILRE RSNVSFRCDK CDTLSHKLES MKISDSKRTQ RTKKEKDIAP DMCKNNTSTN
     HCNHRPKIGS FMGKAREACT LPFLIGAAPV CYGVPTGIIS KKDL
//