ID A0A1U7WPM5_NICSY Unreviewed; 755 AA.
AC A0A1U7WPM5;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN Name=LOC104228488 {ECO:0000313|RefSeq:XP_009779256.1};
OS Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4096 {ECO:0000313|Proteomes:UP000189701, ECO:0000313|RefSeq:XP_009779256.1};
RN [1] {ECO:0000313|Proteomes:UP000189701}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA Peitsch M.C., Ivanov N.V.;
RT "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT tomentosiformis.";
RL Genome Biol. 14:R60.1-R60.17(2013).
RN [2] {ECO:0000313|RefSeq:XP_009779256.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_009779256.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily.
CC {ECO:0000256|ARBA:ARBA00006637}.
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DR RefSeq; XP_009779256.1; XM_009780954.1.
DR AlphaFoldDB; A0A1U7WPM5; -.
DR SMR; A0A1U7WPM5; -.
DR STRING; 4096.A0A1U7WPM5; -.
DR GeneID; 104228488; -.
DR KEGG; nsy:104228488; -.
DR eggNOG; KOG1123; Eukaryota.
DR OrthoDB; 1360679at2759; -.
DR Proteomes; UP000189701; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:InterPro.
DR GO; GO:0006367; P:transcription initiation at RNA polymerase II promoter; IEA:InterPro.
DR CDD; cd18029; DEXHc_XPB; 1.
DR CDD; cd18789; SF2_C_XPB; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR032438; ERCC3_RAD25_C.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001161; XPB/Ssl2.
DR InterPro; IPR032830; XPB/Ssl2_N.
DR NCBIfam; TIGR00603; rad25; 1.
DR PANTHER; PTHR11274:SF17; DNA HELICASE; 1.
DR PANTHER; PTHR11274; RAD25/XP-B DNA REPAIR HELICASE; 1.
DR Pfam; PF16203; ERCC3_RAD25_C; 1.
DR Pfam; PF13625; Helicase_C_3; 1.
DR Pfam; PF04851; ResIII; 1.
DR PRINTS; PR00851; XRODRMPGMNTB.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000313|RefSeq:XP_009779256.1};
KW Helicase {ECO:0000313|RefSeq:XP_009779256.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000313|RefSeq:XP_009779256.1};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000189701}.
FT DOMAIN 277..441
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 496..640
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..38
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 755 AA; 85962 MW; 90D0E65AABF5602E CRC64;
MGIGEKDRPN KKIKFSIRPA IIEPEDEEEE EEENGDNDSD SELLIHDSEN KGVELMRDFT
KLELKPDHGN RPLWVCSDGR VFLETYSPLY KQASDFLIAI AEPVSRPQLI HEFNLTPHSL
YAAVSVGLHT HTIISVFNKL SKTKLPEEVV DFIQASTSNY GKVKLVLKQN RYLIESPCIE
VWEKLLKDDV ISRARITSSD LGLLPELMSE EKEARFSFEI DPAQVESVKQ RCLPNALNYP
MLEEYDFEND TVNPDLDMEL KVNAQPRPYQ EKSLSKMFGN GRARSGIIVL PCGAGKSLVG
VSAATRIKKS CLCLATNAVS VDQWAYQFRL WSTIRDEQIC RFTCDSKEQF RGDIAAGVVV
TTYNMIAFGG KRSEESEKLI EEIRNREWGL LLLDEVHVVP AQMFRKVISL TKSHCKLGLT
ATLVREDERI TDLNFLIGPK LYEANWSDLA KGGFIANVQC AEVWCPMTKE FFAEYLKQEN
QRKRQALYVM NPNKFRACEF LIRFHEQQRA DKIIVFSDNL FALMEYAMKL RKPMIYGGTS
HAERMKILEA FRTSPEVNTI FLSKVGDNSI DIPEANVIIQ ISSHAGSRRQ EAQRLGRILR
AKGRPLGRSA IEEHDAFFYS LVSTDTQEMY YSTKRQQFLI DQGYSFKVIT SLPPPDSGPE
LSYYHVEEQL GLLGKVLSAG EDMVGLEEIE EEDTDGKALR RANRSVGSMS TYSGARGMIY
MEYNGGQKKH GGFVQTKSKP KDLSKRHFLF KKRYV
//
