ID A0A1U7WYD1_NICSY Unreviewed; 467 AA.
AC A0A1U7WYD1;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN Name=LOC104231087 {ECO:0000313|RefSeq:XP_009782321.1};
OS Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4096 {ECO:0000313|Proteomes:UP000189701, ECO:0000313|RefSeq:XP_009782321.1};
RN [1] {ECO:0000313|Proteomes:UP000189701}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA Peitsch M.C., Ivanov N.V.;
RT "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT tomentosiformis.";
RL Genome Biol. 14:R60.1-R60.17(2013).
RN [2] {ECO:0000313|RefSeq:XP_009782321.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_009782321.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; XP_009782321.1; XM_009784019.1.
DR AlphaFoldDB; A0A1U7WYD1; -.
DR STRING; 4096.A0A1U7WYD1; -.
DR GeneID; 104231087; -.
DR KEGG; nsy:104231087; -.
DR eggNOG; ENOG502QPV6; Eukaryota.
DR OrthoDB; 930987at2759; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000189701; Unplaced.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF167; 3-KETOACYL-COA SYNTHASE; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000189701};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 6..24
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 162..183
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 26..313
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 333..414
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT ACT_SITE 169
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 248
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 335
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 339
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 368
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 372
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 467 AA; 52919 MW; CDB90A2C5AC69806 CRC64;
MDLISNLFYG LPFFYLLFLI WKIIDRKRHQ NCYILDYECH KPTDDRMLST KFSGEVIRRN
KHLGLNEYQF LLKAIVSSGI GEQTYAPRMV FYGREACPTY EDGILEMEEF FHDSIDKVLK
RNGFSPSDID VLVVNISMLT CMPSLAGRII NYYKMREDIK VYNITGMGCS ASVIAINIVQ
SIFKNEKNKV ALMVTSESLS PNWYTGNNRS MILANCLFRS GGSAILLTNK VALKNKAMFK
LKCLVRTHHG AKDEAYDSCI QKEDDQGHIG FHLDKTLPKA ATKALVDNLK TIAPLVLPVR
ELLRFAIVSL VRKMNWGSTK GGARPVINFK TGVDHICLHT GGKAVIDGVG TNLNLSEYDL
EPARMTLHRF GNTSASSLWY VLGYMEAKKR LKKGDKVLMI SFGAGFKCNS CLWEVLRDLG
NENVWKDCID NYPPKTLANP FLEKFGWLHN EDPNTFVVPE DYVIPDD
//
