ID   A0A1U7XHS3_NICSY        Unreviewed;       929 AA.
AC   A0A1U7XHS3;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   SubName: Full=Uncharacterized protein LOC104236872 isoform X2 {ECO:0000313|RefSeq:XP_009789211.1};
GN   Name=LOC104236872 {ECO:0000313|RefSeq:XP_009789211.1};
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096 {ECO:0000313|Proteomes:UP000189701, ECO:0000313|RefSeq:XP_009789211.1};
RN   [1] {ECO:0000313|Proteomes:UP000189701}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA   Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA   Peitsch M.C., Ivanov N.V.;
RT   "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT   tomentosiformis.";
RL   Genome Biol. 14:R60.1-R60.17(2013).
RN   [2] {ECO:0000313|RefSeq:XP_009789211.1}
RP   IDENTIFICATION.
RC   TISSUE=Leaf {ECO:0000313|RefSeq:XP_009789211.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC       {ECO:0000256|ARBA:ARBA00010871}.
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DR   RefSeq; XP_009789211.1; XM_009790909.1.
DR   AlphaFoldDB; A0A1U7XHS3; -.
DR   GeneID; 104236872; -.
DR   OrthoDB; 659795at2759; -.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.20; -; 2.
DR   Gene3D; 3.30.470.20; ATP-grasp fold, B domain; 2.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR000291; D-Ala_lig_Van_CS.
DR   InterPro; IPR011095; Dala_Dala_lig_C.
DR   InterPro; IPR011127; Dala_Dala_lig_N.
DR   InterPro; IPR016185; PreATP-grasp_dom_sf.
DR   PANTHER; PTHR23132; D-ALANINE--D-ALANINE LIGASE; 1.
DR   PANTHER; PTHR23132:SF0; D-ALANINE-D-ALANINE LIGASE FAMILY; 1.
DR   Pfam; PF07478; Dala_Dala_lig_C; 2.
DR   Pfam; PF01820; Dala_Dala_lig_N; 2.
DR   SUPFAM; SSF56059; Glutathione synthetase ATP-binding domain-like; 2.
DR   SUPFAM; SSF52440; PreATP-grasp domain; 2.
DR   PROSITE; PS50975; ATP_GRASP; 2.
DR   PROSITE; PS00843; DALA_DALA_LIGASE_1; 1.
DR   PROSITE; PS00844; DALA_DALA_LIGASE_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00409}; Cell shape {ECO:0000256|ARBA:ARBA00022960};
KW   Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00409}; Peptidoglycan synthesis {ECO:0000256|ARBA:ARBA00022984};
KW   Reference proteome {ECO:0000313|Proteomes:UP000189701}.
FT   DOMAIN          186..431
FT                   /note="ATP-grasp"
FT                   /evidence="ECO:0000259|PROSITE:PS50975"
FT   DOMAIN          860..925
FT                   /note="ATP-grasp"
FT                   /evidence="ECO:0000259|PROSITE:PS50975"
SQ   SEQUENCE   929 AA;  102087 MW;  2D20460185A3FD7C CRC64;
     MASSFSINSI NPNTKYLSHL RNYSFPASVL RRTINFPVKK SSNLSCKRRS CRVGIVRASS
     STEVVDDRGS ADVERAHNGK VLRVGLICGG PSAERGISLN SARSVLDHIQ GDDLDVSCYY
     IDSNLNAYAI STAQVYSNTP ADFDFKLESL AQGFRSLSDF TEHLASSVDI VFPVIHGRFG
     EDGGVQELLE RSNIPFVGTG STHCQKAFDK YDASLELDRQ GFVTVPNFLI QVKPTRAGSS
     IGVSVAYGVT DSLQKANTII SEGIDDKVLV EIFLEGGSEF TAIVLDVGSG FGCQPVVLLP
     TEVELQSHGT VDVREKDAIF NYRRKYLPTR QVAYHTPPRF SVDVISKIRE GASLLFQRLG
     LRDFARIDGW FLPPSMKASS FAGNKFGRTN SGTVIFTDIN LISGMEQTSF LFQQASKVGF
     SHSNILRTII QHACLRFPAL LSHNIISSPS RRRSKSASVT EAFIKQHKKV YVIFGGDTSE
     RQVSLMSGTN VWLNLRASDD LEVTPCLLAP ATSYSDVSDF GKHEVDKKFK TVWTLPYSLL
     LRHTTEEVLD ACLEAIEPNR AALTSHLRNQ VMDDLTRGLR KLSWFNGFDI SDELPKKFSL
     EQWVKLAKES QATVFIAVHG GIGEDGTLQS LLEAEGVPYT GPGVIASKTC MDKVATSLAL
     KHLTDFGVLT INKDARKKDD LLKMSISDLW RDLKSKLHCD TLCVKPARDG CSTGVARLCC
     EGDLAFYINA LQDCLPRIPP NSLSKAHGMI EMPNPPPELI IFEPFVETDE IVVASKSRNE
     IAHNLLWKGD SRWVEVTVGV VGKRGSMHSL TPSVTVKESG GILSLEEKFQ GGTGINLTPP
     PPSIMSSSAL ERCKKHIELI ANTLQLEGFS RIDAFVHADT GEVLIIEVNT VPGMTPSTVL
     IHQALAEQPP LYPQQFFRTL LDLASERSM
//