ID   A0A1U7XNV8_NICSY        Unreviewed;       417 AA.
AC   A0A1U7XNV8;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   27-MAR-2024, entry version 30.
DE   RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093};
DE            EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
GN   Name=LOC104238604 {ECO:0000313|RefSeq:XP_009791306.1};
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096 {ECO:0000313|Proteomes:UP000189701, ECO:0000313|RefSeq:XP_009791306.1};
RN   [1] {ECO:0000313|Proteomes:UP000189701}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA   Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA   Peitsch M.C., Ivanov N.V.;
RT   "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT   tomentosiformis.";
RL   Genome Biol. 14:R60.1-R60.17(2013).
RN   [2] {ECO:0000313|RefSeq:XP_009791306.1}
RP   IDENTIFICATION.
RC   TISSUE=Leaf {ECO:0000313|RefSeq:XP_009791306.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC       {ECO:0000256|RuleBase:RU369093}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC         ECO:0000256|RuleBase:RU369093};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
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DR   RefSeq; XP_009791306.1; XM_009793004.1.
DR   AlphaFoldDB; A0A1U7XNV8; -.
DR   SMR; A0A1U7XNV8; -.
DR   STRING; 4096.A0A1U7XNV8; -.
DR   GeneID; 104238604; -.
DR   KEGG; nsy:104238604; -.
DR   eggNOG; ENOG502QR5G; Eukaryota.
DR   OrthoDB; 5489991at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR045185; PUB22/23/24-like.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR22849:SF130; RING-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR   PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000189701};
KW   Transferase {ECO:0000256|RuleBase:RU369093};
KW   Ubl conjugation pathway {ECO:0000256|RuleBase:RU369093}.
FT   DOMAIN          5..90
FT                   /note="U-box"
FT                   /evidence="ECO:0000259|PROSITE:PS51698"
SQ   SEQUENCE   417 AA;  47507 MW;  2C5A83D484F8CF98 CRC64;
     MEEVEIPQYF LCPISLMIMK DPVTTVTGIT YDRESIEMWL LTAEEEAEEE AEAVTCPVTK
     QKLPRDTELL TPNHMLRRLI QAWCIANASK GIDRIPTPKY PLDKSHMLRL VRQVNNHQIC
     IEALRKIEAL VIENEKNKKC LEEVGAIKAM VSFIVKSFKE KKLINGLEHA LRIFHLVWSP
     TTKNEQLVKE NHDLIEAILW ILRSEMMNNN QVIIKTHAMM VLKNVIEISS SNLLSGLQPE
     FFQEMVNILR KESKHHISQQ ATKVALQVLI DTCPWGRNKH KIIESGAIFE LIELELCNTE
     KRVSELIFCI LAHLCTCADG RAEFLKHAAG ISVITKRTLR VSSTTDDCAI QILGSISKFS
     ATKEVLIEML RVGAVSKLCM VIQADCEDYL KKKAMEILRT HSYAWGNSPC IQIHLLT
//