ID   A0A1U7YUL1_NICSY        Unreviewed;       643 AA.
AC   A0A1U7YUL1;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   27-MAR-2024, entry version 30.
DE   RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE            EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN   Name=LOC104248000 {ECO:0000313|RefSeq:XP_009802470.1};
OS   Nicotiana sylvestris (Wood tobacco) (South American tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4096 {ECO:0000313|Proteomes:UP000189701, ECO:0000313|RefSeq:XP_009802470.1};
RN   [1] {ECO:0000313|Proteomes:UP000189701}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23773524; DOI=10.1186/gb-2013-14-6-r60;
RA   Sierro N., Battey J.N., Ouadi S., Bovet L., Goepfert S., Bakaher N.,
RA   Peitsch M.C., Ivanov N.V.;
RT   "Reference genomes and transcriptomes of Nicotiana sylvestris and Nicotiana
RT   tomentosiformis.";
RL   Genome Biol. 14:R60.1-R60.17(2013).
RN   [2] {ECO:0000313|RefSeq:XP_009802470.1}
RP   IDENTIFICATION.
RC   TISSUE=Leaf {ECO:0000313|RefSeq:XP_009802470.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   RefSeq; XP_009802470.1; XM_009804168.1.
DR   AlphaFoldDB; A0A1U7YUL1; -.
DR   GeneID; 104248000; -.
DR   KEGG; nsy:104248000; -.
DR   eggNOG; KOG0167; Eukaryota.
DR   OrthoDB; 2721872at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000189701; Unplaced.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.20.930.20; Adaptor protein Cbl, N-terminal domain; 1.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR036537; Adaptor_Cbl_N_dom_sf.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR23315; U BOX DOMAIN-CONTAINING; 1.
DR   PANTHER; PTHR23315:SF262; U-BOX DOMAIN-CONTAINING PROTEIN 15-LIKE; 1.
DR   Pfam; PF00514; Arm; 2.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00185; ARM; 4.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000189701};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT   DOMAIN          268..342
FT                   /note="U-box"
FT                   /evidence="ECO:0000259|PROSITE:PS51698"
FT   REPEAT          403..440
FT                   /note="ARM"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
FT   REGION          1..25
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..16
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   643 AA;  72389 MW;  BC8EB9005BCCE2D1 CRC64;
     MAKAREHEHE EAVTVEEEFG SSCQTSPQDK RELVRELIGV IQTVGSYEEY RKSQREECHN
     LVERLKLLLP LLVEIRDFDG QITESGIECL MKLKKAFSSA KKLLKTCYCG SKIYLALESE
     AVMGRFHSVY ERLSQALEGM PYDELGISDE EKEQVELLRV QFRRAKTRND TQDMELAMDL
     IVALSTNYDR NVDSASIDRL GHRLGLHTVK ELRAETISVR KVIRERKGRH AEGTQKIIDL
     LNKFRRFAGL EETEALVPKD HEKSTSLAIP NEFLCPITLE IMTDPVIVST GQTYERENIQ
     QLLDSNCRTC PKTGQSLDHL SFTQNFALKN LIQQWCEKNN FQLPKKQPPP KPESPSVESE
     GKFLSLIQDL SSCHLEVQRK AVTEIRMLSK ENPENRRSIL NSGGIPPLVQ LLSYPDSRVQ
     EHAVTTLLNL SIDVTNKRLI SEEKPIPAII EILKNGNLGA KENSAAALFS LSMLDENKEA
     IGSFNGIPPL IDLLRNGTIR GKKDAITALF NLCFNEQNVS LATEAGIVAP LFQLLEKKNL
     DMVDEALSIL LLLATHQDGR QEMGKLSFIE TLVNLIRDGT PKNKECAVAM LHRLSIHNSN
     LMLAALQYGV YEHLVEIADS GTDRGQRNAK SLLQLMSKAE QIP
//