GenomeNet

Database: UniProt
Entry: A0A1U7Z8V9_NELNU
LinkDB: A0A1U7Z8V9_NELNU
Original site: A0A1U7Z8V9_NELNU 
ID   A0A1U7Z8V9_NELNU        Unreviewed;       807 AA.
AC   A0A1U7Z8V9;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   31-JUL-2019, entry version 17.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   Name=LOC104591664 {ECO:0000313|RefSeq:XP_010248912.1,
GN   ECO:0000313|RefSeq:XP_010248913.1, ECO:0000313|RefSeq:XP_010248914.1,
GN   ECO:0000313|RefSeq:XP_010248915.1, ECO:0000313|RefSeq:XP_010248916.1,
GN   ECO:0000313|RefSeq:XP_010248917.1};
OS   Nelumbo nucifera (Sacred lotus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Proteales; Nelumbonaceae; Nelumbo.
OX   NCBI_TaxID=4432 {ECO:0000313|Proteomes:UP000189703, ECO:0000313|RefSeq:XP_010248915.1};
RN   [1] {ECO:0000313|Proteomes:UP000189703}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. China Antique {ECO:0000313|Proteomes:UP000189703};
RX   PubMed=23663246; DOI=10.1186/gb-2013-14-5-r41;
RA   Ming R., VanBuren R., Liu Y., Yang M., Han Y., Li L.T., Zhang Q.,
RA   Kim M.J., Schatz M.C., Campbell M., Li J., Bowers J.E., Tang H.,
RA   Lyons E., Ferguson A.A., Narzisi G., Nelson D.R., Blaby-Haas C.E.,
RA   Gschwend A.R., Jiao Y., Der J.P., Zeng F., Han J., Min X.J.,
RA   Hudson K.A., Singh R., Grennan A.K., Karpowicz S.J., Watling J.R.,
RA   Ito K., Robinson S.A., Hudson M.E., Yu Q., Mockler T.C., Carroll A.,
RA   Zheng Y., Sunkar R., Jia R., Chen N., Arro J., Wai C.M., Wafula E.,
RA   Spence A., Han Y., Xu L., Zhang J., Peery R., Haus M.J., Xiong W.,
RA   Walsh J.A., Wu J., Wang M.L., Zhu Y.J., Paull R.E., Britt A.B., Du C.,
RA   Downie S.R., Schuler M.A., Michael T.P., Long S.P., Ort D.R.,
RA   Schopf J.W., Gang D.R., Jiang N., Yandell M., dePamphilis C.W.,
RA   Merchant S.S., Paterson A.H., Buchanan B.B., Li S., Shen-Miller J.;
RT   "Genome of the long-living sacred lotus (Nelumbo nucifera Gaertn.).";
RL   Genome Biol. 14:R41-R41(2013).
RN   [2] {ECO:0000313|RefSeq:XP_010248912.1, ECO:0000313|RefSeq:XP_010248913.1, ECO:0000313|RefSeq:XP_010248914.1}
RP   IDENTIFICATION.
RG   RefSeq;
RL   Submitted (MAY-2017) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   RefSeq; XP_010248912.1; XM_010250610.1.
DR   RefSeq; XP_010248913.1; XM_010250611.1.
DR   RefSeq; XP_010248914.1; XM_010250612.1.
DR   RefSeq; XP_010248915.1; XM_010250613.1.
DR   RefSeq; XP_010248916.1; XM_010250614.1.
DR   RefSeq; XP_010248917.1; XM_010250615.1.
DR   GeneID; 104591664; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000189703; Genome assembly.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR040881; Urease_linker.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   Pfam; PF18473; Urease_linker; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000189703};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000189703}.
FT   DOMAIN      369    807       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    560    560       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       374    374       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       376    376       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       457    457       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       457    457       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       486    486       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       512    512       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       600    600       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     459    459       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     457    457       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   807 AA;  86919 MW;  3D7B85D6A2BFBC6C CRC64;
     MGSMSSRVWR RSVLTRQMEL DHVICGIGDW DTLLIKRRQV LPAVPHLLDV IQVEGTFLDG
     TKLITVHDAI ASENGNLELA LHGSFLPVPS LDKFSGKDIC TSPGEIIYEV GNITLNAHRR
     AVILLVTNTA DRPVQIGSHY HFIEANPYLI FDRKRAYGMR LNILAGTATR FEPGETKRVT
     LVSIGGHKVI KGGNGIADGA VDVSRIAEVM KAVHDRQFGN AEEIEAREGV TGEDPDLTTI
     VSHETYANMY GPTIGDRIRL GDTELYAEIE RDFTMYGDEC VFGGGKVLRD GMGQASGHPS
     SDCLDTVITN AVIVDYTGIY KADIGIKGGF IICIGKAGNP DVMDGVSSNM TIGVNTEVVA
     GEGMIVTAGA IDCHVHFICP QLAYEAISSG ITTLVGGGTG PADGTRATTC TPAPLQMKLM
     LQSTDEMPLN FGFTGKGNSA NPDGLPEIIR AGAMGLKLHE DWGSTPAAID NCLSVAEEND
     IQVNIHTDTL NESGCVEHTI AAFKERTIHT YHSEGAGGGH APDIIKVCGL KNVLPSSTNP
     TRPFTTNTVD EHLDMLMVCH HLDKDIPEDI SFAESRIREE TIAAEDILHD MGAISIISSD
     SQAMGRIGEV ITRTWQTAHK MKAQRGSIDQ SDTLNDNFRI KRYIAKYTIN PAIANGFSEY
     VGSVEAGKLA DLVLWKPSFF GAKPEMVIKG GAIAWANMGD PNASIPTPQP VMMRPMFGAF
     GKAGSSNSIA FVSKAAKDAG IEKEYGLKKR VVAVGNVRRL TKLDMKLNSA LPVIDVDPEK
     YTVTADGEVL TCTAATTVPL SRNYFLF
//
DBGET integrated database retrieval system