ID A0A1U8BDK5_NELNU Unreviewed; 556 AA.
AC A0A1U8BDK5;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=5'-nucleotidase domain-containing protein DDB_G0275467 isoform X2 {ECO:0000313|RefSeq:XP_010278001.1};
GN Name=LOC104612312 {ECO:0000313|RefSeq:XP_010278001.1};
OS Nelumbo nucifera (Sacred lotus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Proteales; Nelumbonaceae; Nelumbo.
OX NCBI_TaxID=4432 {ECO:0000313|Proteomes:UP000189703, ECO:0000313|RefSeq:XP_010278001.1};
RN [1] {ECO:0000313|RefSeq:XP_010278001.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR017434-2};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR017434-2};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000256|ARBA:ARBA00009589}.
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DR RefSeq; XP_010278001.1; XM_010279699.2.
DR AlphaFoldDB; A0A1U8BDK5; -.
DR GeneID; 104612312; -.
DR OrthoDB; 3626840at2759; -.
DR Proteomes; UP000189703; Unplaced.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07522; HAD_cN-II; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR NCBIfam; TIGR02244; HAD-IG-Ncltidse; 1.
DR PANTHER; PTHR12103; 5'-NUCLEOTIDASE DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR12103:SF12; 5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 1; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR017434-2};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR017434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000189703}.
FT ACT_SITE 117
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT ACT_SITE 119
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT BINDING 117
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 119
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 400
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
SQ SEQUENCE 556 AA; 64059 MW; AC93955C116F8F90 CRC64;
MASIRRLLPF CSSLQRTGFS LRGTAEGFGV GGSCRSFSAT VASEEAIFKC PDEDGRVEPE
VLVMEDEIAK IRREFEAAKQ SFLKIPEALR EMPKMNPRGI FVNKNLRLDS VQVYGFDYDY
TLAYYSSNLQ NLIYDLAKEH LVNEFRYPES CLGFKYDPSF PIRGLYYDKL KGCLLKLDFF
GSIESDGCFF GRRKLSKKEI EEIYGTRHIG RDQAHGLVGL MDFFCFSEAC LIADIVQHFV
DAKLEFDACY IYQDVNRAIQ HVHHSGLVHR GILSDPQKYL VKNGQMLRFL KMLKEKGKKL
FLLTNSPYYF VDGGMRFMLE DAIGSTDSWR ELFDVVIARA NKPGFYNSEH PFRCYDIEKD
TLAFSRVDAF LPNQVYYHGC LKSFLQITRW KGPEVIYFGD HLFSDLRGPS KAGWRTAAII
HELENEICIQ NGENYRFHQA KFHIIQELLG KFHATVANSQ RGEAYDSLLN ELNMERKMAR
SVMKNMFNSS FGATFLTDTG QESAFAYHIH QYADVYTSKP ENFLLYSHEA WLHVPFDIKI
IPHHVKVPSS SFKTNQ
//
