ID A0A1U8HLI4_GOSHI Unreviewed; 668 AA.
AC A0A1U8HLI4;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=alpha-amylase {ECO:0000256|ARBA:ARBA00012595};
DE EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595};
DE AltName: Full=1,4-alpha-D-glucan glucanohydrolase {ECO:0000256|ARBA:ARBA00030238};
GN Name=LOC107887239 {ECO:0000313|RefSeq:XP_016666912.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016666912.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016666912.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016666912.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR RefSeq; XP_016666912.1; XM_016811423.1.
DR AlphaFoldDB; A0A1U8HLI4; -.
DR STRING; 3635.A0A1U8HLI4; -.
DR PaxDb; 3635-A0A1U8HLI4; -.
DR OrthoDB; 201664at2759; -.
DR Proteomes; UP000189702; Chromosome 12.
DR GO; GO:0004556; F:alpha-amylase activity; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005987; P:sucrose catabolic process; IBA:GO_Central.
DR CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR012850; A-amylase_bs_C.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF33; ALPHA-AMYLASE 3, CHLOROPLASTIC; 1.
DR Pfam; PF07821; Alpha-amyl_C2; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SMART; SM00810; Alpha-amyl_C2; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..30
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 31..668
FT /note="alpha-amylase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010590226"
FT DOMAIN 276..605
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT DOMAIN 606..666
FT /note="Alpha-amylase C-terminal beta-sheet"
FT /evidence="ECO:0000259|SMART:SM00810"
SQ SEQUENCE 668 AA; 75228 MW; 189941B3DA899441 CRC64;
MIQAKSSFGK WPVIYRRILC ILLILDAVEQ LPNMVLNVEA SCASKNENRR LEGFYEEQPI
VKEVSVGNMV NVAIRKFPDA ANIVLHLETD IPGDVVVHWG VCRDDAKTWE IPAVTYPPET
VVFRNKALRT LLQPTGNGSR AFFTLDEKLD GFLFVLKLDD NSWVNFQGND FYIPLSSARS
VKGQSNSESE ETSGKAHADG IINEIRNLIS GLNSEKSLQT KVKEAKESIL NEIEKLAAEA
YSIFRSSTPS YPEEDSDADD AEPTINISSG TGSGFEILCQ VFNWESNKSG RWYMELKEKA
SEISSLGFTV IWLPTPTESV SPEGYMPKDL YNLNSRYGTI DELKDHVKSL HGVGLKVLGD
VVLNHRCAHY QNQNGIWNIF GGRLNWDGRA VVGDDPHFQG RGNKSSGDNF HAAPNIDHSQ
DFVRKDLIEW LCWLREEIGY DGCRLDFVRG FWGGYVKDYL DASEPYFAVG EYWDSLSYTY
GEMDHNQDAH RQRIVDWINA TNGTAGAFDV TTKGILHSAL ERREYWRLSD QKGKPPGVVG
WWPSCAVTFI ENHDTGSTQG HWRFPGGKEM QGYAYILTHP GTPAVFYDHI VSHHRSEIGA
LISLRNRNKI HCRSIVEIVK AERDVYAATI DDRVAMKIGP GYYEPPSGPQ IWSLALEGYD
YKVWEASS
//
