ID A0A1U8HLI9_GOSHI Unreviewed; 641 AA.
AC A0A1U8HLI9;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=alpha-amylase {ECO:0000256|ARBA:ARBA00012595};
DE EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595};
DE AltName: Full=1,4-alpha-D-glucan glucanohydrolase {ECO:0000256|ARBA:ARBA00030238};
GN Name=LOC107887239 {ECO:0000313|RefSeq:XP_016666915.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016666915.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016666915.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016666915.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
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DR RefSeq; XP_016666915.1; XM_016811426.1.
DR AlphaFoldDB; A0A1U8HLI9; -.
DR OrthoDB; 201664at2759; -.
DR Proteomes; UP000189702; Chromosome 12.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR012850; A-amylase_bs_C.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF33; ALPHA-AMYLASE 3, CHLOROPLASTIC; 1.
DR Pfam; PF07821; Alpha-amyl_C2; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR SMART; SM00642; Aamy; 1.
DR SMART; SM00810; Alpha-amyl_C2; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000189702}.
FT DOMAIN 249..578
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT DOMAIN 579..639
FT /note="Alpha-amylase C-terminal beta-sheet"
FT /evidence="ECO:0000259|SMART:SM00810"
SQ SEQUENCE 641 AA; 72092 MW; D69909A1BBB82E48 CRC64;
MIQAKSSFGK WPASCASKNE NRRLEGFYEE QPIVKEVSVG NMVNVAIRKF PDAANIVLHL
ETDIPGDVVV HWGVCRDDAK TWEIPAVTYP PETVVFRNKA LRTLLQPTGN GSRAFFTLDE
KLDGFLFVLK LDDNSWVNFQ GNDFYIPLSS ARSVKGQSNS ESEETSGKAH ADGIINEIRN
LISGLNSEKS LQTKVKEAKE SILNEIEKLA AEAYSIFRSS TPSYPEEDSD ADDAEPTINI
SSGTGSGFEI LCQVFNWESN KSGRWYMELK EKASEISSLG FTVIWLPTPT ESVSPEGYMP
KDLYNLNSRY GTIDELKDHV KSLHGVGLKV LGDVVLNHRC AHYQNQNGIW NIFGGRLNWD
GRAVVGDDPH FQGRGNKSSG DNFHAAPNID HSQDFVRKDL IEWLCWLREE IGYDGCRLDF
VRGFWGGYVK DYLDASEPYF AVGEYWDSLS YTYGEMDHNQ DAHRQRIVDW INATNGTAGA
FDVTTKGILH SALERREYWR LSDQKGKPPG VVGWWPSCAV TFIENHDTGS TQGHWRFPGG
KEMQGYAYIL THPGTPAVFY DHIVSHHRSE IGALISLRNR NKIHCRSIVE IVKAERDVYA
ATIDDRVAMK IGPGYYEPPS GPQIWSLALE GYDYKVWEAS S
//
