ID   A0A1U8HTQ8_GOSHI        Unreviewed;       412 AA.
AC   A0A1U8HTQ8;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   27-MAR-2024, entry version 29.
DE   RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093};
DE            EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE   AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
GN   Name=LOC107889448 {ECO:0000313|RefSeq:XP_016669356.1};
OS   Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX   NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016669356.1};
RN   [1] {ECO:0000313|Proteomes:UP000189702}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX   PubMed=25893780; DOI=10.1038/nbt.3208;
RA   Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA   Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA   Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA   Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA   Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT   "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT   provides insights into genome evolution.";
RL   Nat. Biotechnol. 33:524-530(2015).
RN   [2] {ECO:0000313|RefSeq:XP_016669356.1}
RP   IDENTIFICATION.
RC   TISSUE=Leaf {ECO:0000313|RefSeq:XP_016669356.1};
RG   RefSeq;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC       {ECO:0000256|RuleBase:RU369093}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC         ECO:0000256|RuleBase:RU369093};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
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DR   RefSeq; XP_016669356.1; XM_016813867.1.
DR   AlphaFoldDB; A0A1U8HTQ8; -.
DR   STRING; 3635.A0A1U8HTQ8; -.
DR   PaxDb; 3635-A0A1U8HTQ8; -.
DR   GeneID; 107889448; -.
DR   KEGG; ghi:107889448; -.
DR   OrthoDB; 5489991at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000189702; Chromosome 13.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR045185; PUB22/23/24-like.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR22849:SF132; E3 UBIQUITIN-PROTEIN LIGASE PUB23; 1.
DR   PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS51698; U_BOX; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW   Transferase {ECO:0000256|RuleBase:RU369093};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|RuleBase:RU369093}.
FT   DOMAIN          5..80
FT                   /note="U-box"
FT                   /evidence="ECO:0000259|PROSITE:PS51698"
SQ   SEQUENCE   412 AA;  45458 MW;  3B0F53416643AFCB CRC64;
     MDEIDVPPFF ICPISLEIMK DPVTLSTGIT YDRESIEKWL FSGKNTTCPV TKQVIADCEL
     TPNHTLRRLL QSWCMLNASH GIERIPTPKP PISKAQIVKL LIDAKSPQQQ IKCLDTLKSI
     AAENATNKRC MESSGAVEFL VSIVCNYNPT AAEESLDYEF DSIRLIDEAL STLYSLQLSE
     ASLKNLMGKN GDFILSLTRV MQRGSYESRA YAVLLLKSMA EIADPMQLIN FRPQLFIELV
     QVLHDQISKQ ASKVTLQLLV SICPWGRNKI KAAEAGVVPV LIDLLLDSSS ERRACEMILT
     VLDAVCGCAE GRSELLKHGA GLAIVSKKIL RVSQVASERA VRILLSIAKF GATSKVLQEM
     QQLGVVAKLC LVLQLDCGSK TKEKAREVLK LHATVWKNSP CIPTNLVSSY PA
//