ID A0A1U8HY84_GOSHI Unreviewed; 1099 AA.
AC A0A1U8HY84;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN Name=LOC107889099 {ECO:0000313|RefSeq:XP_016668908.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016668908.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016668908.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016668908.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR RefSeq; XP_016668908.1; XM_016813419.1.
DR AlphaFoldDB; A0A1U8HY84; -.
DR STRING; 3635.A0A1U8HY84; -.
DR PaxDb; 3635-A0A1U8HY84; -.
DR GeneID; 107889099; -.
DR KEGG; ghi:107889099; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000189702; Chromosome 13.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0032543; P:mitochondrial translation; IBA:GO_Central.
DR GO; GO:0009791; P:post-embryonic development; IEA:UniProt.
DR GO; GO:0048608; P:reproductive structure development; IEA:UniProt.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.10.830; Ile-tRNA synthetase CP2 domain-like; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR InterPro; IPR010663; Znf_FPG/IleRS.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF06827; zf-FPG_IleRS; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702}.
FT DOMAIN 129..778
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 823..986
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT DOMAIN 1056..1084
FT /note="Zinc finger FPG/IleRS-type"
FT /evidence="ECO:0000259|Pfam:PF06827"
SQ SEQUENCE 1099 AA; 123710 MW; 2B93E471E96C80DC CRC64;
MSFFKSVAAN SSVFTPREAK MAMRQPSTYG VLSQRAGSSI RKTTSVNLLY FRGSSSVRVF
SFLNIARYSS CSGEEFYSSS KRRSRGPVMA AKKASQGQKE EEGRYKHTVD LPKTTFGMRA
NALVREPEIQ KLWDDHQVFK RVVDKNDGLL FQGNFVLHDG PPYANGDLHM GHALNKILKD
IINRYKLLQN YKVHFVPGWD CHGLPIELKV LQSLDQDARK DLTPLKLRAK AAKFAKATVK
AQMSSFQRFG VWADWNNPYL TLDPKYEAAQ IEVFGEMALK GHIYRGRKPV HWSPSSSTAL
AEAELEYPEG HVSRSIYALF RMVSTPSSKE SLLEEFFPDI CLAIWTTTPW TVPANAAVAV
NAKLQYAVVE AKSLSEDVHL SAGNKKKRLG NIVTEPKMPF FIVASDLVPT LEAKWGIKLI
VKKTLSGSDL ENCRYIHPIN NMECPVVIGG DYITTESGTG LVHTAPGHGQ EDYVTGLKYG
LPIYSPVDDD GKFTEEAGQF NGLDVLGDGN AAVVKYLDET LSIIMEESYE HKYPYDWRTK
KPTIFRATEQ WFASVEGFRQ AAMDAIGHVK WIPAQAQNRI SSMTSSRSDW CISRQRTWGL
PIPVFYHVTS REPLMNKETI DHIKSIIAQK GSDAWWYMTV EDLLPEEYCD TASEYEKGTD
TMDVWFDSGS SWAAVLGERD CLNFPADLYL EGTDQHRGWF QSSLLTSIAT KGKAPYSSVI
THGFVLDEKG LKMSKSLGNV VDPHTIIEGG KNQKEAPGYG ADIMRLWVSS VDYTGDVMIG
PQILRQISDV YRKLRGTLRY LLGNLHDWKV ENAVSYQELP MIDRHALFQL ENVVKNIREG
YENYQFFKIF QIIQRFVNVD LSNFYFDIAK DRLYVGGTTS FTRRSCQTVL AEHLLSLSRV
IAPILPHLAE DVWQNLPFQY TIKDGSIAGF VFESKWPSVN EKWLTFPAEE VDFWGKVLEL
RTEVNKVLEI ARTGKLIGSS LEAKVYLHVS DASLASRLLE MCSANNDADA LHRIFLTSQV
EVVPSLDHEL VQNISHTGEY VVEDDRVWIG VSRADGSKCE RCWNYSPQVG SFTEHPTLCG
RCFNVVGIQP TPAMAAAIS
//
