ID A0A1U8IG94_GOSHI Unreviewed; 526 AA.
AC A0A1U8IG94;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Glutamate--cysteine ligase, chloroplastic {ECO:0000256|ARBA:ARBA00015722};
DE EC=6.3.2.2 {ECO:0000256|ARBA:ARBA00012220};
DE AltName: Full=Gamma-ECS {ECO:0000256|ARBA:ARBA00032122};
DE AltName: Full=Gamma-glutamylcysteine synthetase {ECO:0000256|ARBA:ARBA00030585};
GN Name=LOC107896479 {ECO:0000313|RefSeq:XP_016677150.1,
GN ECO:0000313|RefSeq:XP_016677151.1, ECO:0000313|RefSeq:XP_016677152.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016677150.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016677150.1, ECO:0000313|RefSeq:XP_016677151.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016677150.1,
RC ECO:0000313|RefSeq:XP_016677151.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione from
CC L-cysteine and L-glutamate: step 1/2. {ECO:0000256|ARBA:ARBA00005006}.
CC -!- SUBUNIT: Homodimer or monomer when oxidized or reduced, respectively.
CC {ECO:0000256|ARBA:ARBA00011153}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
CC -!- SIMILARITY: Belongs to the carboxylate-amine ligase family.
CC Glutamate--cysteine ligase type 2 subfamily.
CC {ECO:0000256|ARBA:ARBA00010253}.
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DR RefSeq; XP_016677150.1; XM_016821661.1.
DR RefSeq; XP_016677151.1; XM_016821662.1.
DR RefSeq; XP_016677152.1; XM_016821663.1.
DR STRING; 3635.A0A1U8IG94; -.
DR PaxDb; 3635-A0A1U8IG94; -.
DR GeneID; 107896479; -.
DR KEGG; ghi:107896479; -.
DR OrthoDB; 294549at2759; -.
DR UniPathway; UPA00142; UER00209.
DR Proteomes; UP000189702; Chromosome 16.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0042398; P:cellular modified amino acid biosynthetic process; IEA:InterPro.
DR GO; GO:0006750; P:glutathione biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0043436; P:oxoacid metabolic process; IEA:UniProt.
DR Gene3D; 3.30.590.20; -; 1.
DR InterPro; IPR035434; GCL_bact_plant.
DR InterPro; IPR006336; GCS2.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR011556; Glut_cys_lig_pln_type.
DR NCBIfam; TIGR01436; glu_cys_lig_pln; 1.
DR PANTHER; PTHR34378; GLUTAMATE--CYSTEINE LIGASE, CHLOROPLASTIC; 1.
DR PANTHER; PTHR34378:SF1; GLUTAMATE--CYSTEINE LIGASE, CHLOROPLASTIC; 1.
DR Pfam; PF04107; GCS2; 1.
DR PIRSF; PIRSF017901; GCL; 1.
DR SUPFAM; SSF55931; Glutamine synthetase/guanido kinase; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157,
KW ECO:0000256|PIRSR:PIRSR017901-50};
KW Glutathione biosynthesis {ECO:0000256|ARBA:ARBA00022684};
KW Ligase {ECO:0000313|RefSeq:XP_016677150.1,
KW ECO:0000313|RefSeq:XP_016677151.1};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DISULFID 190..410
FT /evidence="ECO:0000256|PIRSR:PIRSR017901-50"
FT DISULFID 353..368
FT /evidence="ECO:0000256|PIRSR:PIRSR017901-50"
SQ SEQUENCE 526 AA; 59192 MW; FA29460986D3BDA4 CRC64;
MALVSKAGSS YCIGSDITRG RTGQNVAFGV VNNAEAPIMK EKWVNFSSLS CKNARVAPFF
PLETVGFRSK RRNGVIVAAS PPTEDALIAT EPLTKQDLVG YLASGCKSKE KWRIGTEHEK
FGFEIKTLRP MKYEQIAELL NGISERFDWE KVMEGDKIIG LKQGKQSISL EPGGQFELSG
APLETLHQTC AEVNSHLYQV KAVAGEMGIG FLGIGFQPKL GLKDIPVMPK GRYEIMRNYM
PKVGSLGLDM MFRTCTVQVN LDFSSEADMI RKFRAGLALQ PIATALFANS PFTEGKPNGY
LSMRSQIWTD TDKDRTGMLP FVFDDSFGFE QYVDYALDVP MYFVYQKKKY IDCTGMTFRD
FMAGKLPCIP GELPNLNDWE NHLTTIFPEV RLKRYLEMRG ADGGPWRRLC ALPAFWVGLL
YDEVSLQSIL DMTADWTCEE REMLRNKVPK TGLKTPFRDG LLWHIAEDVL KLAKDGLERR
GFKESGFLNE VAEVVRTGVT PAEKLLELYH GKWGQSVDPV FEELLY
//
