ID A0A1U8IJT6_GOSHI Unreviewed; 970 AA.
AC A0A1U8IJT6;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN Name=LOC107895337 {ECO:0000313|RefSeq:XP_016676134.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016676134.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016676134.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016676134.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Through the carboxylation of phosphoenolpyruvate (PEP) it
CC forms oxaloacetate, a four-carbon dicarboxylic acid source for the
CC tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003774}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346}.
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DR RefSeq; XP_016676134.1; XM_016820645.1.
DR AlphaFoldDB; A0A1U8IJT6; -.
DR STRING; 3635.A0A1U8IJT6; -.
DR PaxDb; 3635-A0A1U8IJT6; -.
DR OrthoDB; 355614at2759; -.
DR Proteomes; UP000189702; Chromosome 15.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IBA:GO_Central.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR GO; GO:0048366; P:leaf development; IBA:GO_Central.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF33; PHOSPHOENOLPYRUVATE CARBOXYLASE 3; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702}.
FT ACT_SITE 183
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 612
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 970 AA; 110933 MW; A135535BD3592CB7 CRC64;
MASFNNNNNN NNNNGKFEKL ASIDAQLRQL VPAKVSEDDK LVEYDALLLD RFLDILQDLH
GEDLKETVQE CYELSAEYEG KNNPKKLEEL GNVLTSLDPG DSIVVAKAFS HMLNLANLAE
EVQIAHRRRI KLKKGDFVDE NNATTESDLE ETLKRLVVDL KKSPQEVFDA LKNQTVDLVF
TAHPTQSVRR SLLQKHGRIR NCLAQLYAKD ITPDDKQELD EALGREIQAA FRTDEIRRTP
PTPQDEMRAV MSYFHETVWK GVPKFLRRVD TALKNIGINE RVPYNAPLIQ FSSWMGGDRD
GNPRVTPEVT RDVCLLARMM AANLYYSQIE DLMFELSMWR CSDELRVRAD ELHRSLRRDA
KHYIEFWKQI PPSEPYRVIL GDVRDKLYQT RERSRQLLSH GMSEIPEEAT FTNIEQFLEP
LELCYRSLCC CGDRPIADGS LLDFLRQVST FGLSLVRLDI RQESDRHTDV LDAITKHLEI
GSYREWSEEH QKQEWLLSEL SGKRPLFGSD LPKTEEIADI LDTFNVLAEL PADNFGAYII
SMATAPSDVL AVELLQRECH VKQPLRVVPL FEKLADLEAA PAALARLFSV DWYRNRINGK
QEVMIGYSDS GKDAGRLSAA WQLYKAQEEL IKVAKQFGMK LTMFHGRGGT VGRGGGPTHL
AILSQPPETI HGSLRVTVQG EVIEQSFGEE HLCFRTLQRF TAATLEHGMH PPVSPKPEWR
ALMDEMAIVA TEEYRSIVFK EPRFVEYFRL ATPELEYGRM NIGSRPSKRK PSGGIESLRA
IPWIFAWTQT RFHLPVWLGF GAAFKHAIEK DIKNLQMLQE MYNEWPFFRV TIDLVEMVFA
KGDPGIAGLY DKLLVSPELW SFGERLRTNY EETKCLLLQI AAHKDLLEGD PYLKQRLRLR
DSYITTLNVC QAYTLKRIRD PNYNVKFRPH ICKPADELVK LNPTSDYAPG LEDTLILTMK
GIAAGMQNTG
//
