ID A0A1U8J065_GOSHI Unreviewed; 953 AA.
AC A0A1U8J065;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
GN Name=LOC107902034 {ECO:0000313|RefSeq:XP_016683752.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016683752.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016683752.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016683752.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
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DR RefSeq; XP_016683752.1; XM_016828263.1.
DR AlphaFoldDB; A0A1U8J065; -.
DR GeneID; 107902034; -.
DR OrthoDB; 167550at2759; -.
DR Proteomes; UP000189702; Chromosome 18.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03363; TOPRIM_TopoIA_TopoI; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR HAMAP; MF_00952; Topoisom_1_prok; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005733; TopoI_bac-type.
DR InterPro; IPR028612; Topoisom_1_IA.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034149; TOPRIM_TopoI.
DR NCBIfam; TIGR01051; topA_bact; 1.
DR PANTHER; PTHR42785:SF1; DNA TOPOISOMERASE; 1.
DR PANTHER; PTHR42785; DNA TOPOISOMERASE, TYPE IA, CORE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 326..441
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT REGION 203..315
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..264
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 953 AA; 106206 MW; 372B1C91DE4E0A14 CRC64;
MQRCLLSSPP TSNCAALRLH PSLMPKFQLK VVKLISSIPS QPRFKKLRPP SSLMPSYPNF
ATLHSASTFA LPPNHITYTL PRNRNVISTC VTPQAMRSTS AFVPLSKMFF SHSPPVATKN
DGMSVRDGKS SGFSFRRFHR HWKKLKGVAA LNGDRAAPFE SPASTSLLNS AKPDSTSYSL
VNTANPDSTS TSLVINAKPE SKASLIKKKQ SRSKNKKEQV SSTVVSEETP VAKSSKTSSL
VGTSNISKKN GQRPKQTSEK VSTGNLLAEA PDASASTKKQ SKKNSTGSGR KRQPVADVNE
PVQKQVQKNI SQREKPLKQL YPPAAKSVMV VESVTKAKVI QGYLGDMFEV LPSYGHVRDL
AARSGSVRPD DDFSMVWEVP SAAWTHLKSI KVALSGAENL VLASDPDREG EAIAWHIIEM
LQQQDALGEN VSVARVVFHE ITEASIKSAL QMPREIDVNL VHAYLARRAL DYLIGFNISP
LLWRKLPGCQ SAGRVQSAAL SLICDRETEI DEFKPLEYWT IEVRFEEPNS FPAHLTHFNS
KKLGQFSISS DTEAKDVRQK INIENFKVIN SKRNTMRRNP PAPYITSTLQ QDAANKLNFP
ATYTMKLAQK LYEGIQLSDG KATGLITYSR TDGLHISDEA VKDIRSLVME RYGSRFTPEN
ACKYYKKVKN AQEAHEAIRP TNIRRLPSML VGALDEDSLK LYTLIWSRTM ACQMEPSILE
QIQLETGNCD ESIIFRSSCS RVAFLGYQSA YQDVESETIR FKDNEASHNA VAFGILSSLK
KGDQLSIGEV EIKQHHTQAP PRYSEGSLVK KLEELGIGRP STYASTLKVL QDRSYVTVKS
RVLYPEFRGR MVSAFLSHHF SEVTDYSFTA DMESELDNVS AGLTEWKGLL RDYWTRFSSY
CSRAESVHVH QVEKMLEKTF GDFLFASLPN KSRTCPSFLS LRTCFVYMGV ASR
//
