ID A0A1U8JD05_GOSHI Unreviewed; 789 AA.
AC A0A1U8JD05;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Isoamylase 1, chloroplastic-like {ECO:0000313|RefSeq:XP_016686119.1};
GN Name=LOC107904305 {ECO:0000313|RefSeq:XP_016686119.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016686119.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016686119.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016686119.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
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DR RefSeq; XP_016686119.1; XM_016830630.1.
DR AlphaFoldDB; A0A1U8JD05; -.
DR STRING; 3635.A0A1U8JD05; -.
DR PaxDb; 3635-A0A1U8JD05; -.
DR GeneID; 107904305; -.
DR KEGG; ghi:107904305; -.
DR OMA; HAFVNDQ; -.
DR OrthoDB; 610at2759; -.
DR Proteomes; UP000189702; Chromosome 19.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0043170; P:macromolecule metabolic process; IEA:UniProt.
DR CDD; cd11326; AmyAc_Glg_debranch; 1.
DR CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR048650; ISOA1-3-like_C.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21156; ISOA1-3_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 227..662
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 789 AA; 89285 MW; 35ACED9C92EC1119 CRC64;
MDLIRCTSCF QHSPTLIAPT FRHQTTRKPN LTTISNNHGT VSTTRMVVKA RGGGAAEAET
ALVDKPKVLK RFQVSEGHPA PFGATIQDCG VNFAIYSANS TSATLCLIAL SDLQQNRATE
QIPLDPLANK TGDVWHVFLK GNFKDMLYGY IFDGEFSPEI GHYYDSSRIL LDPYAKAVIS
RGEFGALGPE DNCWPQMAGM VPTSEYQFDW EGDLPLRHPD RDLIIYEMHV RGYTKHESSG
TKFPGTYCGV VEKLDHLKEL GVNCIELMPC QEFNELEYYS YNSVLGDYKL NFWGYSTINY
FSPMIRYSSS GIRSCGRDAI NEFKYLVKEA HKRGIEVIMD VVFNHTAEGN EKGLSLSFRG
VDNCVYYMLA PKGEYYNYSG CGNTFNCNHP VVRQFILDCL RYWVTEMHVD GFRFDLASIM
TRSSSLWDSV NVYGDPLEGD LITTGTPLSN PPLIDMMSND PVLHGVKLIA EAWDAGGLYQ
VGRFPHWGVW SEWNGKYRDI VRQFIKGTDG LSGAFAECLC GSPNLYQEGG GKPWNSVNFI
CAHDGFTLAD LVTYNNKHNL ANGEDNNDGE SHNYSWNCGH EGEFASISVK KLRKRQMRNF
FLCLMVSQGV PMIHMGDEYG HTKGGNNNTY CHDNYINYFR WDKKEESSSD LFRFFHLMTK
FRRECESLGL NDFPTAERLQ WHGHTPGKPD WSDTSRFVAF TLIDSVKGEI YVAFNTSHLP
VTITLPERPG YRWEPLVDTS KPAPFDFLSN EVPERDLAIK QYAHFLDANL YPMLSYSSIV
LLLSPDENA
//
