ID A0A1U8K9W5_GOSHI Unreviewed; 829 AA.
AC A0A1U8K9W5;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891, ECO:0000256|RuleBase:RU362092};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891, ECO:0000256|RuleBase:RU362092};
GN Name=LOC107914775 {ECO:0000313|RefSeq:XP_016699287.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016699287.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016699287.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016699287.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Introduces a single-strand break via transesterification at a
CC target site in duplex DNA. Releases the supercoiling and torsional
CC tension of DNA introduced during the DNA replication and transcription
CC by transiently cleaving and rejoining one strand of the DNA duplex. The
CC scissile phosphodiester is attacked by the catalytic tyrosine of the
CC enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme
CC intermediate and the expulsion of a 3'-OH DNA strand.
CC {ECO:0000256|RuleBase:RU362092}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213,
CC ECO:0000256|RuleBase:RU362092};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446, ECO:0000256|RuleBase:RU362092}.
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DR RefSeq; XP_016699287.1; XM_016843798.1.
DR AlphaFoldDB; A0A1U8K9W5; -.
DR GeneID; 107914775; -.
DR OrthoDB; 166270at2759; -.
DR Proteomes; UP000189702; Chromosome 22.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR11390:SF20; DNA TOPOISOMERASE 3-BETA-1; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362092};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU362092};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Signal {ECO:0000256|SAM:SignalP};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029,
KW ECO:0000256|RuleBase:RU362092}.
FT SIGNAL 1..16
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 17..829
FT /note="DNA topoisomerase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010580745"
FT DOMAIN 1..108
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT REGION 792..829
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 829 AA; 93258 MW; 85610A50C48EBC18 CRC64;
MGSFLAFMPI IKLLQLLDMY SDFPASYQDW NATNPLDLFQ ASVVKTEANP RAHISKHLSQ
EARGCGYLVL WLDCDREGEN ICFEVIECTR FPMNEAKKRV YRARFSSVTE KDILKAMDNL
VEPNRHEALA VDARQEIDLK VGVAFTRFQT SYFQGKYGNL DSRVISYGPC QTPTLGFCVQ
RYLQINTFKP EKFWALRPYI IQNGYELQLE WKRQKLFDID VAMMFQKLVT EDGILEVIDV
SEKQETKGRP SGLNTVNLLK VASSALGYGP QTAMQIAERL YTQGFISYPR TESTAYPSSF
DFKGTLKVLE NNPSWSDCVQ RLLAEGYHKP RTGTDAGDHP PITPMRPATE DVLGNDAWRL
YQYICQHFMG TVSPDCKYVR TKVEFSIGGE FFHCIGQRVT VQGFTYIMPW LAINEKNLPQ
FTKGEKIEVS RVELYEGETS PPDYLSESEL ISLMEKNGIG TDASIPVHIN NICERNYVQV
QAGRRLVPTT LGITLIRGYQ CIDPDLCLPD IRSFIEHQIT LVAKGQANHS RVVQHVLQQF
KQKFCYFLQQ IENMDALFEA QFSPLADSGR ALSKCGKCLR YMKYISAQPQ RMFCGTCEEV
YYLPQKGTIK LYKELTCPLD NFELLIFSMP GPEGKSFPLC PYCYNSPPFE GIGTLFGSAK
IGSTGKLGKG AGMPCFLCLH PTCRHSVVAQ GVCACPECSG TLVLDPVSAP KWRLYCNKCN
CLVLLPEGAH RIATTRDRCA ECDSTIIEVD FNKKTTPLED GATLHTGCIL CDELLHSLVE
VKHGKSFSRG FGARGRGRGR GTGRGRGGYR GTGRTAGKKV DPRMSFRDF
//
