ID A0A1U8M219_GOSHI Unreviewed; 738 AA.
AC A0A1U8M219;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN Name=LOC107932238 {ECO:0000313|RefSeq:XP_016719693.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016719693.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016719693.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016719693.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR RefSeq; XP_016719693.1; XM_016864204.1.
DR AlphaFoldDB; A0A1U8M219; -.
DR PaxDb; 3635-A0A1U8M219; -.
DR GeneID; 107932238; -.
DR KEGG; ghi:107932238; -.
DR OrthoDB; 5489808at2759; -.
DR Proteomes; UP000189702; Unplaced.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF63; BETA-GALACTOSIDASE 12; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 2.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..35
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 36..738
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010552725"
FT DOMAIN 44..348
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 371..427
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 479..546
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT DOMAIN 628..714
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 738 AA; 82055 MW; 834D96977FAE1638 CRC64;
MWAKHNMLSS TTTRANMLFM LWLLFSSWVC SLVSATVSYD SKAIIINGRR RILLSGSIHY
PRSTPEMWPD LIAKAKEGGL DVIQTYVFWN GHEPSPGKYY FEDRYDLVRC VKLVQQAGLY
VNLRIGPYVC AEWNFGGFPV WLKYVPGIAF RTDNGPFKAA MQKFTAKIVN MMKAEKLFQT
QGGPIIMAQI ENEFGPVEWE IGAPGKSYTK WAAQMAVGLR TGVPWIMCKQ DDAPDPVINT
CNGFYCENFT PNAKYKPKMW TENWTGWFTE FGGAVPTRPA EDIAFSVARF IQNGGSFVNY
YMYHGGTNFG RTAGGPFIAT SYDYDAPIDE YGLTREPKWG HLRDLHKAIK SSEAALVSAY
PTVTSLGSKQ EAHVFKSKSG ACAAFLANYD TRYSVKVKFG NGQYDLPRWS ISILPDCKTA
VFNTAKIGAH SSKKAMVPVN SAFSWQSYTE QSPTADDREA TVHNGLWEQI YLTRDASDYL
WYMTDVKIDP NEGFLKGGQG PILTVWSAGH ALHVFTNGQL SGTVYGGLDN PKLTFSNNVK
LRAGINRISL LSVAVGLPNV GTHFETWNAG VLGPVTLKGL NEGTRDISKQ NWSYKIGLKG
EALNLHTVTG SSSVEWVQGS QLVKKQPMTW YKTTFNAPRG NEPLALDMSS MGKGQVWING
QSIGRHWPGY IARGSCGACD YAGTYTDKKC RTNCGEPSQR WYHVPRSWVK PSGNLLVVFE
EWGGDPSRIA LVKRTATA
//
