ID A0A1U8MQL7_GOSHI Unreviewed; 950 AA.
AC A0A1U8MQL7;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Non-lysosomal glucosylceramidase {ECO:0000256|PIRNR:PIRNR028944};
DE Short=NLGase {ECO:0000256|PIRNR:PIRNR028944};
DE EC=3.2.1.45 {ECO:0000256|PIRNR:PIRNR028944};
GN Name=LOC107939039 {ECO:0000313|RefSeq:XP_016727814.1,
GN ECO:0000313|RefSeq:XP_016727816.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016727814.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016727814.1, ECO:0000313|RefSeq:XP_016727816.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016727814.1,
RC ECO:0000313|RefSeq:XP_016727816.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Non-lysosomal glucosylceramidase that catalyzes the
CC hydrolysis of glucosylceramide (GlcCer) to free glucose and ceramide.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine + H2O = an N-
CC acylsphing-4-enine + D-glucose; Xref=Rhea:RHEA:13269,
CC ChEBI:CHEBI:4167, ChEBI:CHEBI:15377, ChEBI:CHEBI:22801,
CC ChEBI:CHEBI:52639; EC=3.2.1.45;
CC Evidence={ECO:0000256|PIRNR:PIRNR028944};
CC -!- SIMILARITY: Belongs to the non-lysosomal glucosylceramidase family.
CC {ECO:0000256|PIRNR:PIRNR028944}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; XP_016727814.1; XM_016872325.1.
DR RefSeq; XP_016727816.1; XM_016872327.1.
DR STRING; 3635.A0A1U8MQL7; -.
DR PaxDb; 3635-A0A1U8MQL7; -.
DR GeneID; 107939039; -.
DR KEGG; ghi:107939039; -.
DR OMA; WNENDEF; -.
DR OrthoDB; 997839at2759; -.
DR Proteomes; UP000189702; Unplaced.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008422; F:beta-glucosidase activity; IBA:GO_Central.
DR GO; GO:0004348; F:glucosylceramidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006680; P:glucosylceramide catabolic process; IEA:InterPro.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR014551; B_Glucosidase_GBA2-typ.
DR InterPro; IPR006775; GH116_catalytic.
DR InterPro; IPR024462; GH116_N.
DR PANTHER; PTHR12654; BILE ACID BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR12654:SF25; NON-LYSOSOMAL GLUCOSYLCERAMIDASE; 1.
DR Pfam; PF04685; DUF608; 1.
DR Pfam; PF12215; Glyco_hydr_116N; 1.
DR PIRSF; PIRSF028944; Beta_gluc_GBA2; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR028944};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR028944};
KW Lipid metabolism {ECO:0000256|PIRNR:PIRNR028944};
KW Membrane {ECO:0000256|PIRNR:PIRNR028944};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702}.
FT DOMAIN 92..408
FT /note="Glycosyl-hydrolase family 116 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12215"
FT DOMAIN 524..884
FT /note="Glycosyl-hydrolase family 116 catalytic region"
FT /evidence="ECO:0000259|Pfam:PF04685"
SQ SEQUENCE 950 AA; 106051 MW; F2DF575D56CA2AF8 CRC64;
MENGVKDTLE NDFTSCSASL EVDPAKPASL TWLRQLNSIG KPPAGFGLSF KEIMYLAPIG
FRLWRYTREE EATGRIPIFD FFRKHLVTCD HGVPLGGIGA GSIGRGYRGE FQRFKLFGTV
CEEGPILANQ FSAFVSRPNG QKYSTVLCAR SPEVTKGSTG SGIESWDWNL KGEKCTYHGL
FPRSWTIYEG EPDPELRISC RQISPFIPHN YKESSLPVSV FTFTLSNTGR TSADVTLLFT
WANSVGGISG FSGDHLNSKM KMENGVRGVL LHHKTAKGQP PLTFAISAKE TNEVHVSECP
CFLISGKSRG ISAKDMWHEI KKNGSFENLS YEECSPSEQG SSIGAAVAAS VTVPPGSDRR
ATFSLAWDCP EVRFGDKTYY KRYSKYYGTL GDAAGNIASD AIFDHSTWES QIESWQRPIL
EDKRLPEWYP VTLFNELYYL NAGGTIWTDG SAPMQSLASL GDRKFSLDKS RSHLDNAINT
ANHKNIAIEI LERMTSTLEK AHIPLTSNSA LGSQLLQDGE ENIGQFLYLE GSEYLMFNTY
DVHFYSSFAL LMLFPKLELS FQRDFAAAVM MHDPSKMEIM YDGKWVPRKI LGAVPHDIGL
NDPWFEVNAY NLFNTDNWKD LNPKFVLQIH RDIVATGDKS FALAVWPSVY TAMAFMDQFD
KDSDGMIENE GFPDQTYDAW PVDGVSAYSG GLWVAALQAT ASIAHQVGDD EAAAYFHTKY
QKAKSVYATL WNGSYFNYDN TANSSSRCIL ADQLAGQWYT GASGLMPIAD DDKVRTALQK
IYDFNVLKVK GGMRGAVNGM LPDGRVDMTG MQSREIWAGV TYALAATMIQ EGMVETAFKT
AAGAHEAAWS QQGLGYSFQT PEAWNTDEEY RSLCYMRPLA IWAMQWALTN PKLSTEETKH
PYGEIDECLY QKQHLAFSKV AHLLKLPNKE ETSKTFLRSL VQFICRRLPI
//
