ID A0A1U8MVH4_GOSHI Unreviewed; 679 AA.
AC A0A1U8MVH4;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN Name=LOC107940604 {ECO:0000313|RefSeq:XP_016729533.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016729533.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016729533.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016729533.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC rhamnogalacturonan I domains in ramified hairy regions of pectin
CC leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC {ECO:0000256|ARBA:ARBA00010418}.
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DR RefSeq; XP_016729533.1; XM_016874044.1.
DR AlphaFoldDB; A0A1U8MVH4; -.
DR STRING; 3635.A0A1U8MVH4; -.
DR PaxDb; 3635-A0A1U8MVH4; -.
DR GeneID; 107940604; -.
DR KEGG; ghi:107940604; -.
DR OrthoDB; 1215403at2759; -.
DR Proteomes; UP000189702; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR CDD; cd10317; RGL4_C; 1.
DR CDD; cd10316; RGL4_M; 1.
DR CDD; cd10320; RGL4_N; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR029413; RG-lyase_II.
DR InterPro; IPR029411; RG-lyase_III.
DR InterPro; IPR010325; Rhamnogal_lyase.
DR PANTHER; PTHR32018:SF6; RHAMNOGALACTURONAN ENDOLYASE; 1.
DR PANTHER; PTHR32018; RHAMNOGALACTURONATE LYASE FAMILY PROTEIN; 1.
DR Pfam; PF14683; CBM-like; 1.
DR Pfam; PF14686; fn3_3; 1.
DR Pfam; PF06045; Rhamnogal_lyase; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE 3: Inferred from homology;
KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|RefSeq:XP_016729533.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 397..469
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14686"
FT DOMAIN 483..671
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14683"
SQ SEQUENCE 679 AA; 79337 MW; 9931FA97D1663944 CRC64;
MEKVVNMSHW RRKLVLWMVM VIQLSLFLSV FSTEIHARKF LKDNDINTND VRLHISDRQV
IIDNGLVEVT IENPSGYLIG IKYKKMQNVL ERRNHISNRG YWDIVWDGDS YDKLETKHVE
VITQTNDIVE LSFTKTWNSK NHGKTIPLNI QKRYIVRRGI SGVYMYGIFE RQEDFPKAHM
YQIRIAFKLK EDKFRFMALS DTIQRVMPRS EDRVKGHSKP LDFKEAVLLT NPSNPKLKGE
VDDKYQYSIE NKDNKLNGWI SDHDSIGFWI MTPSNEFRTG GPHKQDLTSH VGPTALSMFV
STHYTGTEID TFYKEGEAWK KVFGPVLIYL NSASLKDDDH RKILWNDAKR QLSEEIESWP
YNFTRSEDFP HSQQRGQVNG QLLVQDRYMD KQLMQAKSAF VGLAPPGEAD SWQKEGKGYQ
FWTQTDKIGR FNIKNVRPGV YNLYAWVHGF IGSYKLDLNI TIQPENKIEL DTLIYDPPRN
GPTLWEIGIP DRTAAEFFIP EPYPQYVNSI TNDGADKFRQ YGLWDRYSDI YRDSDLVYTV
GTSNYSKDWF FAHVPRKIGD DWYRPTTWQI KYNLQDVNNR GTYTLQMAIA AASFAEVEVR
FNDPNSDRPH FTTRRIGYDN AVPRHGIHGL YRLYSIEVPG YRFLKGSNTI YLTQTRSDNS
FEAVMYDYIR LEGPNSLNN
//
