ID A0A1U8NQH0_GOSHI Unreviewed; 490 AA.
AC A0A1U8NQH0;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=LOC107950779 {ECO:0000313|RefSeq:XP_016741227.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016741227.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016741227.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016741227.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR RefSeq; XP_016741227.1; XM_016885738.1.
DR AlphaFoldDB; A0A1U8NQH0; -.
DR STRING; 3635.A0A1U8NQH0; -.
DR PaxDb; 3635-A0A1U8NQH0; -.
DR GeneID; 107950779; -.
DR KEGG; ghi:107950779; -.
DR OMA; LVFRTCD; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000189702; Chromosome 7.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 26..490
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5010397641"
FT DOMAIN 29..483
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 410
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 461
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 470
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 490 AA; 53529 MW; 838CB0C45C4D5405 CRC64;
MAAPITMLIL CTSFLSLSLL STAFTSRDYA DALEKSILFF EGQRSGPLPS NQRLKWRGNS
GLSDGASYHV DLVGGYYDAG DNVKFGLPMA FTTTMLAWSV IEFGGSMHSQ IGNAKTALRW
ATDYLLKAAT ATPDSLYVQV GDPNMDHKCW ERPEDMDTPR NVYKVSAQNP GSDVAGETAA
ALAAASIVFK DSDPSYSAKL LQTAKNVFDF ADKYRGSYSD SLNSVVCPFY CSYSGYQDEL
LWGASWMHRA TQDSSYLSYI QSNGHTMGSD NDDFSFSWDD KRAGTKVLLS KDFLEKSTSE
FQLYKSHSDN YICSLIPGSS GFQAQYTPGG LLYKASESNL QYVTTTTFLL LTYAKYLSTN
GGVVTCGTST VTAESLIAQA KKQVDYILGD NPAKMSYMVG FGERYPQHVH HRGSSVPSIK
AHPDRINCNN GFQYLYANSP NPNVLAGAVI GGPDSKDNFA DDRNNYQQSE PATYINAPFV
GALAFFSGKA
//
