ID A0A1U8PFB7_GOSHI Unreviewed; 675 AA.
AC A0A1U8PFB7;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN Name=LOC107957840 {ECO:0000313|RefSeq:XP_016748919.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000189702, ECO:0000313|RefSeq:XP_016748919.1};
RN [1] {ECO:0000313|Proteomes:UP000189702}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000189702};
RX PubMed=25893780; DOI=10.1038/nbt.3208;
RA Li F., Fan G., Lu C., Xiao G., Zou C., Kohel R.J., Ma Z., Shang H., Ma X.,
RA Wu J., Liang X., Huang G., Percy R.G., Liu K., Yang W., Chen W., Du X.,
RA Shi C., Yuan Y., Ye W., Liu X., Zhang X., Liu W., Wei H., Wei S., Huang G.,
RA Zhang X., Zhu S., Zhang H., Sun F., Wang X., Liang J., Wang J., He Q.,
RA Huang L., Wang J., Cui J., Song G., Wang K., Xu X., Yu J.Z., Zhu Y., Yu S.;
RT "Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1)
RT provides insights into genome evolution.";
RL Nat. Biotechnol. 33:524-530(2015).
RN [2] {ECO:0000313|RefSeq:XP_016748919.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_016748919.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC rhamnogalacturonan I domains in ramified hairy regions of pectin
CC leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC {ECO:0000256|ARBA:ARBA00010418}.
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DR RefSeq; XP_016748919.1; XM_016893430.1.
DR AlphaFoldDB; A0A1U8PFB7; -.
DR STRING; 3635.A0A1U8PFB7; -.
DR PaxDb; 3635-A0A1U8PFB7; -.
DR GeneID; 107957840; -.
DR KEGG; ghi:107957840; -.
DR OrthoDB; 1215403at2759; -.
DR Proteomes; UP000189702; Chromosome 10.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR CDD; cd10317; RGL4_C; 1.
DR CDD; cd10316; RGL4_M; 1.
DR CDD; cd10320; RGL4_N; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR029413; RG-lyase_II.
DR InterPro; IPR029411; RG-lyase_III.
DR InterPro; IPR010325; Rhamnogal_lyase.
DR PANTHER; PTHR32018:SF8; RHAMNOGALACTURONAN ENDOLYASE; 1.
DR PANTHER; PTHR32018; RHAMNOGALACTURONATE LYASE FAMILY PROTEIN; 1.
DR Pfam; PF14683; CBM-like; 1.
DR Pfam; PF14686; fn3_3; 1.
DR Pfam; PF06045; Rhamnogal_lyase; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE 3: Inferred from homology;
KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|RefSeq:XP_016748919.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000189702};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 394..465
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14686"
FT DOMAIN 480..668
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14683"
SQ SEQUENCE 675 AA; 77579 MW; C7186D80CB476C4A CRC64;
MTSKVRRIID CFSDCCGCKT CKGNARQDML NMNQDTNCDP MLTQGVRLHI QDRYVVMDNG
IIQVSLSKPG GIVTGIWYNG IDNLLEVQNK ETYRGYWDLH WNEIGGKGIF DVIQGTSFRV
IVENEEQVEI SFTRTWNHSL EGKFIPLNID KRFIMLRCSS GFYSYAIYEH FREWPGFELG
ETRITFKLRK DKFQYMAVAD NRQRYMPFPD DRSNGRGVPL AYPEAVLIVN PLDQRLTGEV
DDKYQYSCEN KELRVHGGIC FDPPVGFWQI TPSDEFRSGG PLKQNLSSHV GPTTLAMFLS
SHYAGKYMVP QFEAGEPWKK VFGPIFMYFN SAAYGNDPLL LWEDAKIKMM VEVQSWPYSF
PASEDFPKSE QRGNANGRIL IQDRYISNYC VIASGAYVGL APPGDAGSWQ MESKNYQFWT
QANEDGFFSI RNIRPGDYNL YAWVPGFIGD YRHEAVITII SGCNIEMGDV IYEPPRDGPT
LWEIGIPDRS AAEFYIPDPD PKYINRLFVN HSDRFRQYGL WERYTELYPE GDLVYKIGVS
DYRKDWFFAQ VVRKIGDNAY QGTTWQIKFE LDNVDRNGIY KLRVALASAT LAELQVRVTD
PTSNRPLFTT GLIGRDNAIA RHGIHGIYKL YNVDIPGTRF VKGENTIFFK QPRCNSPFQG
FMYDYIRLEG PSTPC
//
