ID A0A1U9K6H3_9BACL Unreviewed; 697 AA.
AC A0A1U9K6H3;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=B0W44_07340 {ECO:0000313|EMBL:AQS55622.1};
OS Novibacillus thermophilus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Thermoactinomycetaceae;
OC Novibacillus.
OX NCBI_TaxID=1471761 {ECO:0000313|EMBL:AQS55622.1, ECO:0000313|Proteomes:UP000188603};
RN [1] {ECO:0000313|EMBL:AQS55622.1, ECO:0000313|Proteomes:UP000188603}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SG-1 {ECO:0000313|EMBL:AQS55622.1,
RC ECO:0000313|Proteomes:UP000188603};
RX PubMed=25951858; DOI=10.1099/ijs.0.000306;
RA Yang G., Chen J., Zhou S.;
RT "Novibacillus thermophilus gen. nov., sp. nov., a Gram-staining-negative
RT and moderately thermophilic member of the family Thermoactinomycetaceae.";
RL Int. J. Syst. Evol. Microbiol. 65:2591-2597(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
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DR EMBL; CP019699; AQS55622.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1U9K6H3; -.
DR STRING; 1471761.B0W44_07340; -.
DR KEGG; ntr:B0W44_07340; -.
DR OrthoDB; 9803554at2; -.
DR Proteomes; UP000188603; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005738; TopoIII.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR NCBIfam; TIGR01056; topB; 1.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:AQS55622.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000188603};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 2..135
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 697 AA; 80638 MW; E85CD4AC0281879D CRC64;
MKTLVLAEKP SVGRDLARVL GCRQQHKTYI EGSRYIVTWA LGHLVELKMP EDYDKRYETW
RMEDLPIIPE QMELKVIRKT SAQFKAIQKL TKRKDIKELV IATDAGREGE LVARWIIKKI
HWRKPVKRLW ISSQTDRAIR EGFKQLKPAH AYDALYASAV CRAEADWLIG LNVTRALTTK
YDDPLSAGRV QTPTLNIILE REHEIETFVP KEFRTLTAHF GSFSAQWEQN GDKRLFDLER
AEQLSSTLKG EKGVVRSVKQ KTKREPQPLP YDLTELQRDA NRRYGFSAKK TLNILQQLYE
YHKLITYPRT DSRYLTKDME TTMPKRLKAL SDGYRDEVKL LLGRNAPLLA KRVFNDHKVT
DHHAIIPTDE PLVLSDLSAD ERKVYDMIVR RFLALFHPPH RYSVITAVID IAGETFVTHE
TREVERGFTV VMRQERAAAN QSEPLPLKEG QTVVPKDIVM EKGYTEPPAR LNEADLLARM
EKHGLGTPAT RADIIERLLE TEVVTRHNGR FRPTPKGKQL INLVSEELRS PELTSRWERE
LEAIARGKGN AKTFLANIRR QTYTLVNEIK TSDQTYRAYN LTGSKCPECE AKLKEVKRKN
GTMLVCSNRR CTYRRRKDPV LSNRRCPQCR KRMEIHQGKA GPYFQCRSCN LVEKKDRSTG
NVSKREERQL LKKYSGHESL KTNLGEALKA ALQKREE
//
