ID A0A1U9NLS4_9BACT Unreviewed; 720 AA.
AC A0A1U9NLS4;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA {ECO:0000313|EMBL:AQT68859.1};
GN ORFNames=STSP2_02035 {ECO:0000313|EMBL:AQT68859.1};
OS Anaerohalosphaera lusitana.
OC Bacteria; Planctomycetota; Phycisphaerae; Sedimentisphaerales;
OC Anaerohalosphaeraceae; Anaerohalosphaera.
OX NCBI_TaxID=1936003 {ECO:0000313|EMBL:AQT68859.1, ECO:0000313|Proteomes:UP000189674};
RN [1] {ECO:0000313|Proteomes:UP000189674}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ST-NAGAB-D1 {ECO:0000313|Proteomes:UP000189674};
RA Spring S., Bunk B., Sproer C.;
RT "Comparative genomics and description of representatives of a novel lineage
RT of planctomycetes thriving in anoxic sediments.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP019791; AQT68859.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1U9NLS4; -.
DR STRING; 1936003.STSP2_02035; -.
DR KEGG; alus:STSP2_02035; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000189674; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000189674}.
FT DOMAIN 9..285
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 286..558
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 720 AA; 81232 MW; 125C5BDA9AC6C2E9 CRC64;
MTRQAFTTDQ LTESQRRAVF HKDGPLLVIA GPGSGKTRVI TSRIAALIDA GVRPWNICAI
TFTNKAAEEM RERLAAGGGG EGVHTSTFHS LCVRLLRMYA DAAGISNNFS IYDTDDQRRC
VKEAIKNSNI DSKTFTPAKV LSAISNFKND LETPEQILER ADEFYLKTAG RVYEQYQKLL
KKNNALDFDD LLINTAFLLR DNPDIRQALG NRYQYLLVDE YQDTNHAQYQ IAKGLALEHR
NICVTGDPDQ SIYKWRGADI QNILMFEKDW PDAVVVKLEE NFRSTPNILE MADVLIANNT
QRKEKRLVAT HDRGADAEIE CYGDGLEESV EVAGKIQALI EEGADPNEIA VFYRVNSMSR
TVEEAFVRQQ IPYQVVRGVE FYARKEIRDM LSYMKVIANP SDDMALLRAV GTHSRGIGKM
TLSRVQHYAS QYGITMFNAM NQADAIDTIT PRTQTKVKAF AAMIEKWRAD ADGPVAPLME
RVFDESGLLD SLRAGGEDEE SAIENIDELI NSASRYDEMT EEPSLIDYLQ SIALYSDTDA
YDAEAGRVSL MTLHAAKGLE FDNVFIIGLE EGLLPHERSL VGGEEELEEE RRLFFVGITR
ARKVLHISHA RYRTMRGQHL RTTPSKFLYE IGYTPKEEQF ESYTYDTEFS QVAPSGFGSK
IEKKEAPFRT GELVEHRKFG TGRVKEFQDM GDNSIIVVRF QSGQVKTLML KYANLTKLSG
//
