ID A0A1V0A195_9ACTN Unreviewed; 519 AA.
AC A0A1V0A195;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE SubName: Full=Glycoside hydrolase 43 family protein {ECO:0000313|EMBL:AQZ63968.1};
GN ORFNames=BKM31_23130 {ECO:0000313|EMBL:AQZ63968.1};
OS Nonomuraea sp. ATCC 55076.
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Streptosporangiaceae; Nonomuraea.
OX NCBI_TaxID=1909395 {ECO:0000313|EMBL:AQZ63968.1, ECO:0000313|Proteomes:UP000190797};
RN [1] {ECO:0000313|Proteomes:UP000190797}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 55076 {ECO:0000313|Proteomes:UP000190797};
RX PubMed=28626548; DOI=10.1039/c6md00637j;
RA Nazari B., Forneris C.C., Gibson M.I., Moon K., Schramma K.R.,
RA Seyedsayamdost M.R.;
RT "Nonomuraea sp. ATCC 55076 harbours the largest actinomycete chromosome to
RT date and the kistamicin biosynthetic gene cluster.";
RL Med. Chem. Commun. 8:780-788(2017).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
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DR EMBL; CP017717; AQZ63968.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V0A195; -.
DR STRING; 1909395.BKM31_23130; -.
DR KEGG; noa:BKM31_23130; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000190797; Chromosome.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18617; GH43_XynB-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000190797}.
FT DOMAIN 319..514
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 15
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 183
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 124
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 519 AA; 56486 MW; D54D6F6EBCB5A83D CRC64;
MRTFRNPVLP GCHPDPSICR VGADFYLVTS SFAYYPGLPL FHSRDLVRWR RLTHVLNRPG
QLPLQGLDVS DGVWAPTIRH HDGTFYVVST VAVNRQGSIT FVVTATDPAG PWSDPVPLEA
EGIDPSLFFD DDGRCWFTAC RDAAPGGRGP GELWMRELDL ERLKLVGPTH VLWHGAVRGA
WVEAPRLYKR DGVYHLLAAE GGTERNHSVT AARAATVTGP YVTDPRSPLL THRHRGPAEP
VHNVGHADLV DTPDGETWAV TLAVRPIDGT HTLGREVFLV PVTWTEHGPV FAPETGGVRL
TERLPRLAAP FVEEAARGRT NFDGAVPGLE WSSLRGPVTH LIRPAADGRG LEIDAAPEPL
TTTGVPAFIA RRQQHVRCHA TTSLTFPAAH PAEEAGLAVF QNQAHHATLA LTVDEEGHPQ
VALTIREVGT TTRLATTPLP PGKTHLSVES DESRYLFRAR PAGTADWTEV GTVERSFFST
ERAGGFVGVH LGLYATANGR PSTARATVRR FDYEPGLGE
//
