ID A0A1V1REZ9_9ACTN Unreviewed; 462 AA.
AC A0A1V1REZ9;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=Uncharacterized RNA methyltransferase CT0009 {ECO:0000313|EMBL:GAV31094.1};
GN Name=trmA {ECO:0000313|EMBL:GAV31094.1};
GN ORFNames=emb_1c0064 {ECO:0000313|EMBL:GAV31094.1};
OS Coriobacteriaceae bacterium EMTCatB1.
OC Bacteria; Actinomycetota; Coriobacteriia; Coriobacteriales;
OC Coriobacteriaceae.
OX NCBI_TaxID=1927122 {ECO:0000313|EMBL:GAV31094.1, ECO:0000313|Proteomes:UP000191049};
RN [1] {ECO:0000313|EMBL:GAV31094.1, ECO:0000313|Proteomes:UP000191049}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EMTCatB1 {ECO:0000313|EMBL:GAV31094.1};
RA Kobayashi H., Fu Q., Maeda H., Sato K.;
RT "Draft genome sequence of a novel Coriobacteriaceae sp. strain EMTCatB1
RT reconstructed from the metagenome of a thermophilic electromethanogenic
RT biocathode.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAV31094.1}.
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DR EMBL; BDLO01000001; GAV31094.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V1REZ9; -.
DR STRING; 1927122.emb_1c0064; -.
DR Proteomes; UP000191049; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000191049};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 28..87
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 420
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 420
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 301
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 330
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 350
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 393
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 462 AA; 49349 MW; A42743517C890400 CRC64;
MYRHTKPPPE PCSPLETPAP PTGLYHGPVT EGADVRLSIE RLAYGGDGVS VAPDGRTVFV
PSTCPGDVVE AEVIADKGRY LKARALEIVE PSPDRVEPVC PYFGSCGGCQ WQHVSYEKQL
EWKRRAVEDA LARIGHVCAE VQPPVPSPAE YGYRNKIELL PGSADTPLSM GFARLGSSDV
VPVRSCALLP SVRSGLPAAV TGALRFVSGR ADTSISRVAI RVASDGETEV DVWTPPSAFP
RHLAAKTISD ATGARTVTRV VARGPASARD VRKVEVLTGP GYWTERLGED RYVVSAPSFF
QVNTHAAERL RALVVDLSRE LAPVSVVDAY AGVGTFTLPL ARLADVTAIE SSSYALADLR
RNLERARLAV AVVPGDAAHA LAEVRGVDLA LVDPPRTGLS ADALRAVIAT GTPHVLYVSC
DPTTLARDAA RLADAGFRLE RVVPVDLFPQ TYHVETVALF SR
//
