ID A0A1V2ES30_9SPHN Unreviewed; 830 AA.
AC A0A1V2ES30;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Nitrite reductase {ECO:0000313|EMBL:ONF95098.1};
DE EC=1.7.1.4 {ECO:0000313|EMBL:ONF95098.1};
GN Name=nasD {ECO:0000313|EMBL:ONF95098.1};
GN ORFNames=SPHI_27280 {ECO:0000313|EMBL:ONF95098.1};
OS Sphingomonas jeddahensis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=1915074 {ECO:0000313|EMBL:ONF95098.1, ECO:0000313|Proteomes:UP000188729};
RN [1] {ECO:0000313|EMBL:ONF95098.1, ECO:0000313|Proteomes:UP000188729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=G39 {ECO:0000313|EMBL:ONF95098.1,
RC ECO:0000313|Proteomes:UP000188729};
RA Poehlein A., Wuebbeler J.H., Steinbuechel A., Daniel R.;
RT "Genome sequence of Sphingomonas jeddahensis G39.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRNR:PIRNR037149};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- COFACTOR:
CC Name=siroheme; Xref=ChEBI:CHEBI:60052;
CC Evidence={ECO:0000256|ARBA:ARBA00001929};
CC -!- PATHWAY: Nitrogen metabolism; nitrate reduction (assimilation).
CC {ECO:0000256|ARBA:ARBA00005096}.
CC -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain
CC family. {ECO:0000256|ARBA:ARBA00010429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ONF95098.1}.
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DR EMBL; MPSB01000016; ONF95098.1; -; Genomic_DNA.
DR RefSeq; WP_076745488.1; NZ_MPSB01000016.1.
DR AlphaFoldDB; A0A1V2ES30; -.
DR STRING; 1915074.SPHI_27280; -.
DR OrthoDB; 9768666at2; -.
DR UniPathway; UPA00653; -.
DR Proteomes; UP000188729; Unassembled WGS sequence.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0050661; F:NADP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-UniRule.
DR CDD; cd19944; NirB_Fer2_BFD-like_2; 1.
DR Gene3D; 3.30.390.30; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR Gene3D; 3.30.413.10; Sulfite Reductase Hemoprotein, domain 1; 1.
DR Gene3D; 3.90.480.10; Sulfite Reductase Hemoprotein;Domain 2; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR016156; FAD/NAD-linked_Rdtase_dimer_sf.
DR InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR InterPro; IPR036136; Nit/Sulf_reduc_fer-like_dom_sf.
DR InterPro; IPR012744; Nitri_red_NirB.
DR InterPro; IPR017121; Nitrite_Rdtase_lsu.
DR InterPro; IPR006067; NO2/SO3_Rdtase_4Fe4S_dom.
DR InterPro; IPR045854; NO2/SO3_Rdtase_4Fe4S_sf.
DR InterPro; IPR006066; NO2/SO3_Rdtase_FeS/sirohaem_BS.
DR InterPro; IPR041575; Rubredoxin_C.
DR NCBIfam; TIGR02374; nitri_red_nirB; 1.
DR PANTHER; PTHR43809; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR PANTHER; PTHR43809:SF1; NITRITE REDUCTASE (NADH) LARGE SUBUNIT; 1.
DR Pfam; PF04324; Fer2_BFD; 2.
DR Pfam; PF01077; NIR_SIR; 1.
DR Pfam; PF03460; NIR_SIR_ferr; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR Pfam; PF18267; Rubredoxin_C; 1.
DR PIRSF; PIRSF037149; NirB; 1.
DR PRINTS; PR00368; FADPNR.
DR PRINTS; PR00411; PNDRDTASEI.
DR PRINTS; PR00397; SIROHAEM.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 2.
DR SUPFAM; SSF56014; Nitrite and sulphite reductase 4Fe-4S domain-like; 1.
DR SUPFAM; SSF55124; Nitrite/Sulfite reductase N-terminal domain-like; 1.
DR PROSITE; PS00365; NIR_SIR; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|PIRNR:PIRNR037149};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|PIRNR:PIRNR037149}; Heme {ECO:0000256|ARBA:ARBA00022617};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063,
KW ECO:0000256|PIRNR:PIRNR037149};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000313|EMBL:ONF95098.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000188729}.
FT DOMAIN 16..291
FT /note="FAD/NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF07992"
FT DOMAIN 327..392
FT /note="NADH-rubredoxin oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF18267"
FT DOMAIN 432..478
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 493..542
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 568..632
FT /note="Nitrite/Sulfite reductase ferredoxin-like"
FT /evidence="ECO:0000259|Pfam:PF03460"
FT DOMAIN 641..770
FT /note="Nitrite/sulphite reductase 4Fe-4S"
FT /evidence="ECO:0000259|Pfam:PF01077"
FT REGION 805..830
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 830 AA; 89136 MW; FE307F78C45CD3D2 CRC64;
MKHEPIRVAE ADKRPHLIVI GNGMAGCRAV EELLARDPAR YRVTIVGAER HVNYNRIMLS
PVLAGEKTFD DIVINGRDWY DSNGITLIAG DPIVAIDRAA RTVTSRGGIV MHYDRLLIAT
GSDPFIIPVP GKDLTGVISF RDMTDVDAML AAAEKGGSAV VIGGGLLGLE AAHGLSLRGM
TVTVIHLMPT LMERQLDEAA GWLLKTALEA RGQTIMTGAD TAEIVGDGRV EAVRLKDGTE
IPADLVVMAV GIRPSVALAR EAGLEVGRGI KVDDHMVTSD PAILAVGECV EHQGQVYGLV
APLWDMCRAL ADGLVDAPSG YTPAPTSTKL KVAGLDVFSA GDFSGGDGAE DIVLRDASRG
IYKRVVVKED RIVGAVLYGD TADGNWYFDL LRRAESVADI REALIFGQAF ATGGALADPK
AAVAALSEEA EICGCNGVSK GQVAACISMG AHSLDAVRAA CKASASCGSC TGIVEILLAL
TLGGEVEAGP KTMCKCTSFG HDDVRREIVA QGMRSIPEVM QKLHWTTPDG CSSCRPALNY
YLLCALPGDY VDDQQSRFVN ERMHANIQKD GTYSVVPRMW GGLTNPRELR AIADVVEKFN
APMVKVTGGQ RLDIFGIRKE DLPAVWADLN AAGMVSGHAY GKSLRTVKTC VGSEWCRFGT
QDSTGLGVKL ERATWGSWMP HKFKIAVSGC PRNCAEATIK DFGVVCVDSG YELHVGGNGG
IKVRATDLLC KVATEAEAME MCAAFIQLYR EEARYLERTA PWIERVGLPY IQARLLPDAA
ARAELARRFF FSQQFSQDDP WAARVEGGER EQHAPMARFE PVAARQEEMA
//
