ID   A0A1V2H4G8_9PROT        Unreviewed;       791 AA.
AC   A0A1V2H4G8;
DT   07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2017, sequence version 1.
DT   24-JAN-2024, entry version 21.
DE   SubName: Full=Penicillin acylase {ECO:0000313|EMBL:ONG55008.1};
GN   ORFNames=BKE38_09650 {ECO:0000313|EMBL:ONG55008.1};
OS   Pseudoroseomonas deserti.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC   Acetobacteraceae; Pseudoroseomonas.
OX   NCBI_TaxID=1817963 {ECO:0000313|EMBL:ONG55008.1, ECO:0000313|Proteomes:UP000188879};
RN   [1] {ECO:0000313|EMBL:ONG55008.1, ECO:0000313|Proteomes:UP000188879}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=M3 {ECO:0000313|EMBL:ONG55008.1,
RC   ECO:0000313|Proteomes:UP000188879};
RA   Subhash Y., Lee S.;
RT   "Draft Genome sequence of Roseomonas sp. strain M3.";
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC       Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC   -!- SIMILARITY: Belongs to the peptidase S45 family.
CC       {ECO:0000256|ARBA:ARBA00006586}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ONG55008.1}.
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DR   EMBL; MLCO01000077; ONG55008.1; -; Genomic_DNA.
DR   RefSeq; WP_076957164.1; NZ_MLCO01000077.1.
DR   AlphaFoldDB; A0A1V2H4G8; -.
DR   OrthoDB; 9760084at2; -.
DR   Proteomes; UP000188879; Unassembled WGS sequence.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR   CDD; cd03747; Ntn_PGA_like; 1.
DR   Gene3D; 1.10.1400.10; -; 1.
DR   Gene3D; 2.30.120.10; -; 1.
DR   Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR   Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR   InterPro; IPR029055; Ntn_hydrolases_N.
DR   InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR   InterPro; IPR043147; Penicillin_amidase_A-knob.
DR   InterPro; IPR023343; Penicillin_amidase_dom1.
DR   InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR   InterPro; IPR002692; S45.
DR   PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR   PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR   Pfam; PF01804; Penicil_amidase; 1.
DR   PIRSF; PIRSF001227; Pen_acylase; 1.
DR   SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE   3: Inferred from homology;
KW   Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW   Reference proteome {ECO:0000313|Proteomes:UP000188879};
KW   Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT   ACT_SITE        256
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT   BINDING         328
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT   BINDING         331
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ   SEQUENCE   791 AA;  86591 MW;  7D63D335866CAAFC CRC64;
     MNDTVAPPAS PTLPGETIRL RGLQAPASIH VDRWGIPHLR AENEADLFFL QGFNAARDRL
     WQIDLWRKRG LGLLAADFGP GFLAQDRASR LFLYRGDMEA EFSAYAPDAR QICEAFVAGI
     NAFVDLVEAE PARLPAEFRA LGTRPSRWAA EDVVRIRSHS LTRNALSEVL RAIVLSKADL
     ATDLLRKHLE PMVEPVRPEG LDLSTLGTEV LTQFKLATAA VTFSAERLAA PLDDWRRWTA
     VTDLGDVVMG AEMEGSNNWA IHGSRSESGR PILASDPHRA HAAPSLRYIV HLDSPGFNAI
     GAGEPAVPGI SIGHNGTSAF ALTIHGADQE DVYVYETAEG DRNSYRHGDA FSDMDVVRDN
     FAVKGHPDQT LMLRFTQHGP VIAEDVANRR AVAIRTVWSM PGSAPYLASL ASMRATTLEE
     FRTAIRRWGT PSVNKVYADT SGTIAWTPAG FAPQRPNWDG LLPVPGDGRY EWSGLLDPAE
     HPWRVDPLEG FVHSANEQNL PADWNHAEKT YGYEWVEASR ATRIRESLTR GPLLTLADCT
     ALQTDVLSIP ARRLCALLLT LPRGSAPELW AAQTLLEDWD FRLAADSAAA GLFEVFLTKH
     LKPRLLAALV PDATLRPLLL PQDMESLLLV LENPDARLPA AARDALLADC LMAAFHDCES
     RMGSCPSGWS WGKLHHGYFA HALQGLTAET ATLDVGPFPV GGSAQTPMHT GYRPGDFRCT
     FGASVRLVMD VGEWDNSLCI NAPGQSGDPA SPHYADLAPH WAQGEYVPLL YSREAVDRME
     MQRIALVPVA D
//