ID A0A1V2QE21_9PSEU Unreviewed; 1187 AA.
AC A0A1V2QE21;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ALI22I_26335 {ECO:0000313|EMBL:ONI86219.1};
OS Saccharothrix sp. ALI-22-I.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharothrix.
OX NCBI_TaxID=1933778 {ECO:0000313|EMBL:ONI86219.1, ECO:0000313|Proteomes:UP000188617};
RN [1] {ECO:0000313|EMBL:ONI86219.1, ECO:0000313|Proteomes:UP000188617}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ALI-22-I {ECO:0000313|EMBL:ONI86219.1,
RC ECO:0000313|Proteomes:UP000188617};
RX PubMed=28087533;
RA Yeager C.M., Gallegos-Graves V., Dunbar J., Hesse C.N., Daligault H.,
RA Kuske C.R.;
RT "Polysaccharide degradation capability of Actinomycetales soil isolates
RT from a semi-arid grassland of the Colorado Plateau.";
RL Appl. Environ. Microbiol. 0:0-0(2017).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ONI86219.1}.
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DR EMBL; MTQP01000064; ONI86219.1; -; Genomic_DNA.
DR RefSeq; WP_077008210.1; NZ_MTQP01000064.1.
DR AlphaFoldDB; A0A1V2QE21; -.
DR STRING; 1933778.ALI22I_26335; -.
DR Proteomes; UP000188617; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000188617}.
FT DOMAIN 639..800
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 818..975
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1187 AA; 129314 MW; 01EC67BD9A358F25 CRC64;
MPQPGPLSGL LTAVLPDKAL RALADSVGVP DLELEGPPAA RPLVAAALAN SAPVLAVTAT
GREADDLKAV LCDLIGPEQV ALFPSWETLP HERLSPRADT VGARLEVLRR LAHPDGHPLK
VVITTVRSLI QPMAPGLGDL KPVHLTVGGE HDFEALLVNL TEIAYTRVDM VEKRGEFAVR
GGILDVFPPT AEHPLRVEFW GDEVSEIRPF AVADQRSLPQ EISEFIAPPC RELLLTESVK
GKAAVLAEEH AADAHLHELL DKIANGIPAE GMEALIPALC EGELQLLTDV VPEGTHVVLN
DPEKIRARAQ DLVRTGQEFL EASWMAAAGG GQSPIDLDSS AYHSLADVAR AAKAKNRPWW
TLSQLTTEGG DVIRLELKHV EAYQGDIDRA FADLRAHTVS GGTAVLVVPG AGTAQRATEQ
LREADVNVVL RDTLDTAPKP GAVTVVRGAL EDGFSLPELA LVVLTETDLT GGRHGTSTKD
MRRMPSRRRN AVDPLALKAG DYVVHEQHGI GKYVEMVQRT VAGATREYLV LEYGSSKRGQ
PGDRLFVPTD QLDEVSRYVG GELPTLNKLG GSDWKNTKAK AKKAVKQIAA ELVQLYAARQ
AAPGHAFASD TPWQRELEDA FPFTETIDQM AAIDEVKSDM ERTVPMDRVI CGDVGYGKTE
IAVRAAFKAV QDGKQVVVLV PTTLLAQQHL NTFTERMRAF PVNIKGLSRF TDALEAEQTI
TGLAEGDVDI VIGTHRLLQK SLRYKDLGLV IVDEEQRFGV EHKEHIKALR THVDVLTMSA
TPIPRTLEMS LAGIREMSTI LTPPEERHPI LTYVGGYDDK QVGAAIRREL LRDGQVFYVH
NRVSSIERAA RHIRELVPEA RVITAHGQMN EDKLEKIIQG FWENEYDVLV CTTIVETGLD
ISNANTLIVE RGDLLGLAQL HQLRGRVGRG RERGYAYFLY PPEAPLTETA HDRLATIAQN
TELGAGMAVA MKDLEIRGAG NILGAEQSGH IAGVGFDLYV RLVGEAVEAF RKHAGADGEL
EEDLAEVRVD LPVDAHIPHD YVPGERLRLE AYRKIAAAGD AESLQSVWDE LKDRYGTPPL
PVERLLAVAR FRQTCRAHGV TEVATQGATI RFAPLELKDS QLVRLRRLFP KALYKQANGT
VSVPRPTEGA AGGRMGAPQL RDQELLDWCA QFLASLAGTP VSGVTRS
//
