ID A0A1V2QW38_9PSEU Unreviewed; 4522 AA.
AC A0A1V2QW38;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Polyketide synthase {ECO:0008006|Google:ProtNLM};
GN ORFNames=ALI22I_04505 {ECO:0000313|EMBL:ONI92325.1};
OS Saccharothrix sp. ALI-22-I.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharothrix.
OX NCBI_TaxID=1933778 {ECO:0000313|EMBL:ONI92325.1, ECO:0000313|Proteomes:UP000188617};
RN [1] {ECO:0000313|EMBL:ONI92325.1, ECO:0000313|Proteomes:UP000188617}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ALI-22-I {ECO:0000313|EMBL:ONI92325.1,
RC ECO:0000313|Proteomes:UP000188617};
RX PubMed=28087533;
RA Yeager C.M., Gallegos-Graves V., Dunbar J., Hesse C.N., Daligault H.,
RA Kuske C.R.;
RT "Polysaccharide degradation capability of Actinomycetales soil isolates
RT from a semi-arid grassland of the Colorado Plateau.";
RL Appl. Environ. Microbiol. 0:0-0(2017).
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ONI92325.1}.
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DR EMBL; MTQP01000038; ONI92325.1; -; Genomic_DNA.
DR RefSeq; WP_077004283.1; NZ_MTQP01000038.1.
DR STRING; 1933778.ALI22I_04505; -.
DR OrthoDB; 9778690at2; -.
DR Proteomes; UP000188617; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR CDD; cd08952; KR_1_SDR_x; 2.
DR CDD; cd08956; KR_3_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 3.40.50.11460; -; 1.
DR Gene3D; 6.10.140.1830; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF18369; PKS_DE; 1.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 6.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 3.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000188617};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 33..459
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1410..1488
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1504..1925
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2930..3005
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3028..3454
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4363..4441
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 4522 AA; 479943 MW; B3B25C22C34BC8AF CRC64;
MGNEDRLRAY LKRVTADARE LRRRLDEVEE HESEPIAVIG IACRFPGAVA TPEDLWRLVA
QETDAISEFP RDRGWDLDGL IDPDPGVTGK SYTRHGGFLD GADGFDAAFF DIAPREALGM
DPQQRLLLET SWEAVERAGI RPCELSGSAT GVFVGAMTQD YGPRMWRAPE EVAGYLATGN
TGSVLSGRVS YTFGLEGPAL TIDTGCSSSL VALHLAIRAL RHRECSLAFA GGVTVIANPG
VFVEFSRQRG LSPDGRCKAF SASADGTGFA EGVGVLLLER LSDARRLGHP IRGVVRGSAV
NQDGASNGLT APNGPAQQQV IRAALHDAQV SADEVDLMEA HGTGTVLGDP IEAQALQATY
GRNRSADHPL WLGSVKSNIG HTQAAAGVAG VIKVLMAMRY GRMPRTLHVT APSGNVDWSA
GAVRLLTEAR PWERTGSPRR AGVSAFGVGG TNAHVLLEEP GPTAAEDRDE PVVAPAAVAW
PISAKSAAAL RQQAGRLRHH VAENADATPA DVACSLARRG DFAHRAVVVG RDHEELDRGL
SVLNGGGLSD RVVRGIAVPG RKVAFVFPGQ GSQWPGMARE LLATAPPFAD SVRDCASALE
SHVDWSPLDV LSGAPGAPSL ERVDVVQPAL FTVMVSLARL WRSHGVEPDA VVGHSQGEIA
AAYIAGALSL EDAAKVVALR SRALAELAGA GGMAAVSLPA TRLAGLLERW SGRLSIAAAN
GPTATVVAGD PDALEQLLHR LAEDGVRCGR LPVDYASHSP QVERIRSELL RLTADVRAAA
AGVPIYSAVT GDVLDLGALT PEYWYRNLRS TVRFDDVVRK LLDAEHRALV EVSPHPVLTA
AIEETAEDHG VDDAVVVETL RRDDGGLSRF LLSVSRAQVH GVPVDWDAAL NGARARRVDL
PTYPFQHERY WLTDGTPEER PEPDPADSRY RITWKEVGTG SQRLFGTWLV VRTSAADARE
WADEAEGALR ANGADVTALV LDGARTDRTE LSAVLANKFR DERPAGILSL LSFARDPHPG
HPALSTGTAL TLTLVQALAD VDARVPLWVA TSGAVAVHDS ETIGDPEDAR LWGLGIVAAL
EHHEWWGGLV DLPRTAERIP PELWAAVLAA PGGEDQVALR PSGLFARRLV RDDAVRRSWS
SGRPGGVLIT GGAGAAGAEV ARWFAAQGAE HLVLTCDRDG EVADLVAELT ARGPQVTVVT
RDSADRAGFA EQVVRFRATT PVRTVVHAAT HTCLTPLVGL ALDDFAEAVA HRTQEAAELD
ALFPDADDFV VFSSVTGVWG AKDHAADAAA NAHLDALAER RRHRGLAGSS VAWGVWADAA
GTAGRAVSRQ GLPALSTRSV HAALDRIFPR LNASTTVADI RWERFLPVFT TFRRCRLFDE
IPETHQPDCA AEDPGSSSLF AEISRLPSAD RERKLLDFVV AQVAEVLGYA SAASVRSEST
FKELGITSLT AVDLRNRIRE AGGLGLPVTL VFDHPTPAAL AAHLAAAQTG TATEPASPDR
ADPDEPIAIV GMACRFPGGV ASPEDLWRLV SEGVDAISPA PSDRGWDLRG LRFPDGAQGY
AGGFLPDVDG FDAAFFGISP REAVAMDPQQ RLLLEVSWEA VERAGIDPAA LRGSRTGVFI
GTNGQDYVNL VVNEESKGDG FLATGNAASV MSGRLSYTYG LEGPSLTLDT ACSSSLSALH
LACQALRQDE CRLAFAGGVT VMATPESLSE FSRQGVLAPD GRCKAFSANA DGAGFAEGVG
LVLLERLSDA RRNGREVLAV VRGSAVNQDG ASNGLTAPNG PSQQRVIQAA LASGGLSPAD
IDAVEGHGTG TVLGDLVEVQ ALMAAYGRNR AEEQPLWLGS LKSNIGHTQA AAGIAGVIKS
VMSMRHGTVP RTLHVAQPLT TVDWSAGRMR LAAEPVPWPE TGRPRRAAVS SFGMSGTNAH
VVIEHIPHDV NASDRCEDEP RALPWPISAH GDDALHAQAA RLREHVEAHP ELTTADVGWS
LATRRGTLER RAVVIARGRD EFIGALAKVD EAGGQSANVV RGGAPTTGKV VFVFPGQGTQ
WPRMARELMR DSAVFARGIR ACEQALERHV DWSLTAVLDG EPGAAPLDRV DVVQPVLFSV
MVSLAEMWRS HGVHPAAVVG HSQGEVAAAC VAGALSLEDA AKVIALRSRA LREVVGRGAM
ASIALPPDQV RTVAAGQGEG ISIAAVNSPR STVVAGDSAA VDRLLAQLAR DGVRTHRVQV
DYASHSPQVE PVRANLEAVL AGIRPAASGT SFYSTVEGRP IATGELDAGY WYRNLRSEVR
FDRTVRRLLD DGFDVFLEVS PHPVLVTGIQ ETVEDVQADN VVVLGSLRRD EGTGRRFLRS
VAEAHVHGVA VDWEPAFAGM DPRPAPLPTY AFQRRRFWVK PDSPPTHRDA AEATFWNAVE
REDLEAVAET LGVDPEQRET LNRALPMLST WRRRRHEQDI IGRWRYRVGW KPVTGPRESR
LTGEWLLVRP PGEGIDAWAR QVRAHTDEVS ELVVHPGEVE RTALASELAE LRGRHLTGVV
SLLALDEQPH DTFPLVRGLT GTAVLVQALG DAGLEVPLWC VTSGAVKVGE TDAPVSPAQA
GTWGLGLVAA LEHPDLWGGL IDMVGPDAMR WLGAVVTGAE DQVAVRVSGL WARRLVSAGR
GVPRSWRPRG TVLVTGGTGA LGARVARWLA EHGADHLVLA SRRGAGAPGA ARLRAELERA
GAQVTLAACD VGDRAALSDL LNELRERDSR LRAVVHTAGS LRIAPLSETT VADFARVASA
KVGGAMLLDE LLRGEDLDAF VLFSSNAGVW GGGGQGAYAA ANAYLDALAL RRRAHGETAT
SVSWGLWAGG GMGGEEADEE LLRRGVVAME PQRALAALHH AIEQDDDHVA IADIDWDRFV
PAYTSGGRRP LISDLPGARR LLAAPTTTGT ARSRALVEEL SDRSAAGQRG VLVELVRTIA
AAVLGHDDVD GIAHTRPFRD LGVDSLRAVE LRDRLSSATG LRLATTLVFD HPTPAALAEH
LRHALLGSEG ERHRGAEQRR TARCDDSGDP LAIVAMACRF PGGIASPEDL WRVVESGADV
IGAFPRDRGW DLAGLHASDP DRPGTCYVRE GGFLHDAGMF DPELFGISPR EARSMDPQQR
LLLETAWEAF ERAGLDPTAL RGSRTAVFVG ATYQDYGSVV RRDRESKEGH LLTGNSSSVM
SGRVAYTFGL VGPAITVDTA CSSSLVALHL AGDALRRGEC SLALVGSAAV MATPAAFVEF
SRQRGLAPDG RCKSFADTAD GTAWSEGAGA ILLERLSDAR RNGHPVLAIV RGTAVNQDGA
SNGLTAPSGL AQQDVIRAAL ANAGLAPAEV DAVEGHGTGT PLGDPIEAQA VLATYGRQRP
ADQPLWLGSL KSNIGHAQAA AGIAGMVKTV MALRHALLPR SIHLGEPTRQ VDWSAGHVRL
VTDSRPWPQT GRPRRAGVSS FGISGTNAHV ILEQAPDEDP RPREDRSNRS VLPWLLSAKT
PRALRAQGAR LLSFVEGNPD LDLADVGLSL ASSRAGLEHR AAVVGTKRDC FLRGLSALVG
DESAPEVHED VVVEGGVAFL FPGQGAEWPG MGRELYAAFP VFATAVDEVV CALDADIAAD
LREAMFGEAR DLLERTVFAQ AALFAVEVGS FRLLESWGVV PDFVLGHSVG ELAAAHVSGA
LSLTDAARLV SARGRLMQAR LPAGAMVALR ATPDDVLPLL DERVSLAAVN GPSSVVISGD
EDAVAELVDR CTARGWRHRR LSTAHAFHSP HVDLMEDDFE EVASDLSVAE PAIPLVSGTT
GEPVGVRELR RPGHWCRQAR DTVRFYDGVR WLSAQGVRTF MELGPDSVLS AMGAECVEDR
TRSAFIPIMR SGRDAEHTTA RAVAQLHNRG VGVDWRAVFR GTGAREADLP TYAFQRQPYW
AGVPVEREAR EAEEPGGELY RCDWVPISDN GVVEPSSWAF VGSCPEGWAT AVGGTDYPDR
TSLCRAVDTG TDVPQFVVVG VAAATEDDRA EGAHSAAHEV LELVQWWLGD SRWDSSRLVV
VTDGATGTGL AHAPVWGLVR SAQAEHPDRF VLVDRDSGTT APEGLFRAVA SGEKEVVVRG
EEVVVPSWIP VAPPVSPAST DLSGGTVLVT GGTGVLGSCV ARFLVERWGV GSLVLASRRG
PDAPGASDLV VALESSGARV RVVSCDVGSR EEVADLVASV PSGWPLRGVV HAAGTTEDGV
ISGAGPDVVP RVFRAKVTGA WWLHQATAEL DLAFFVFFSS AAGVLGSGGQ GIYAGANAFL
DGLARFRRYR GLPGQSLAWG LWEQRSGLTE RLSRVDDERL ARSGVLPLST EEGLRLFDAA
RAIDEPVLMP IRTNAGLSAE RADIGAAGSG DLARRLASQT PDQRYHTLLR LVGAEVATVL
ALPTAEAVDP QQAFADLGFD SMMAVELRNR LSATTGVRLR ATVVFDHPTA GDLAAHLRSE
LEPDAARPAA HTAVLGRLDQ LETALSELTP DDDGHTAITA RLKRLLTVWT GTGKQPAGGS
PQLDSATDDE VFTLLGRDFG IS
//
