ID A0A1V2YBF7_9FIRM Unreviewed; 733 AA.
AC A0A1V2YBF7;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=ATP-dependent DNA helicase {ECO:0000256|RuleBase:RU364053};
DE EC=5.6.2.4 {ECO:0000256|RuleBase:RU364053};
GN ORFNames=BEN19_03350 {ECO:0000313|EMBL:OOB76920.1};
OS Epulopiscium sp. Nuni2H_MBin003.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Candidatus Epulonipiscium.
OX NCBI_TaxID=1884657 {ECO:0000313|EMBL:OOB76920.1, ECO:0000313|Proteomes:UP000189211};
RN [1] {ECO:0000313|EMBL:OOB76920.1, ECO:0000313|Proteomes:UP000189211}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Nuni2H_MBin003 {ECO:0000313|EMBL:OOB76920.1};
RA Ngugi D.K., Miyake S., Stingl U.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618,
CC ECO:0000256|RuleBase:RU364053};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922, ECO:0000256|RuleBase:RU364053}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OOB76920.1}.
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DR EMBL; MDJN01000202; OOB76920.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V2YBF7; -.
DR STRING; 1884657.BEN19_03350; -.
DR Proteomes; UP000189211; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR01073; pcrA; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA-binding {ECO:0000256|RuleBase:RU364053};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000189211}.
FT DOMAIN 5..286
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 287..560
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 733 AA; 83788 MW; CBB3272AC45ACF86 CRC64;
MDFLSGLNSM QQKAVLHTTG PLLILAGAGS GKTRVLTHRI ANLITNENIN PWEILAITFT
NKAALEMRER IGKLIGEDTL KDMWISTFHS MCVRILRRDG NKIGYNRYFT IYDTSDQKSL
IKDILKLNNI PEKDFPIGTV LSSISSKKNH FILPKQAKQD GKNDYRENIL AQLYDVYQDK
LFENNAMDFD DLLINVCILF QSNTEVLDFY QRKFKYILVD EYQDTNTVQY ELIRLLSNAS
KNLCVVGDDD QSIYGWRGAD IRNILGFEQD FPNTMIIKLE QNYRSTKNIL DAANKVVSTN
KGRKDKTLFT QNDTGEKISV VCVANEYREA DFVANTIVTE IESGNREYKN FAILYRTNAQ
SRVLEEKMIY NDIPYRMLGG VKFYERKEIK DIIAYLRVIT NNQDDVATKR IINVPKRGIG
ATSIQKIDNF AINNNIKFYD CAKNCSQLGV LANASAVKVM SFVQVIEELR DNKDDIVNLI
KRVLEKTDYI NYIKQTESED SQDRIQNIQE FVSKATAYMK NAESATLEGF LEEVALVADI
DNYDSDSNAV ILMTLHSAKG LEFPIVFMPG LEEGLFPSYM SDSDDAKLEE ERRLCYVGIT
RACEKLHILY TNERTVYGQT RANTPSRFIG ELPEDVIETI GAMKRPKSNY ETYRTSYTSP
QKKTFNSQGT YKPPLQKEIP KLYAGDIIKH KKFGRGTIVK MAKMPDNEII ATIEFESGEK
KNLNVKYANL QKG
//
