ID A0A1V4CHN3_9BURK Unreviewed; 1040 AA.
AC A0A1V4CHN3;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000256|ARBA:ARBA00017273, ECO:0000256|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902};
GN ORFNames=BC358_06000 {ECO:0000313|EMBL:OPF64383.1};
OS Hydrogenophaga sp. H7.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Hydrogenophaga.
OX NCBI_TaxID=1882399 {ECO:0000313|EMBL:OPF64383.1, ECO:0000313|Proteomes:UP000189907};
RN [1] {ECO:0000313|Proteomes:UP000189907}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H7 {ECO:0000313|Proteomes:UP000189907};
RA Florea S., Webb J.S., Jaromczyk J., Schardl C.L.;
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|ARBA:ARBA00007391, ECO:0000256|HAMAP-
CC Rule:MF_01902}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OPF64383.1}.
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DR EMBL; MCIC01000012; OPF64383.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V4CHN3; -.
DR STRING; 1882399.BC358_06000; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000189907; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_01902}; Ligase {ECO:0000256|ARBA:ARBA00023146};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022840};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01902}; Reference proteome {ECO:0000313|Proteomes:UP000189907};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01902}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1040 AA; 115335 MW; 83D286D9FAA57919 CRC64;
MSLPDYAELL CCSNFTFLRG ASHAEELVLR AQAMGYTALA VTDECSMAGI ARAHVAAKKH
GLPLIVGSQF LVRADGDPSG DFTLLLLATN LNGYGNLCEL ITRLRRSADK GTYRLRLGDV
NPEILTDCLA IAVPPRGTTQ DQCNATARWL LGTFLGRCWL GVTQLGRLDD AIHLHRLREA
GALTAVPLVA VGDVHMHVRS RKPLQDVLTA IRIGRPLTDC GFALAPNAEQ HLRTRVRLAQ
SFPPELLAET LHIAGCCTKW SLDELCYQYP AEVVPEGRTP AQHLRDLTEA GAAEHWPAGV
PPKVRAQIEH ELALITELGY EHYFLTVHDI VAFARSQGIL CQGRGSAANS AVCYCLGVTA
VDPARSSLLF ERFLSRERNE PPDIDVDFEH ERREEVIQYL YRKYGRDRAA LTATVISYRP
RSTVRDVGKA LGFDDELLER LARDHRWWDE DGLADTRLRE AGLDPDSLAA RQFVTLVKQL
LGFPRHLSQH TGGFVLTSGP LARLVPIENA SMPDRTVIQW DKDDLDAMRL LKVDVLALGM
LTAIRKSLDF IGQRRGRPMP MHTIPDGDEA TYDMICAADT VGVFQIESRA QMAMLPRLRP
RKFYDLVVEV ALVRPGPIQG GAVHPYLRRR QGKEDVDYPK GLEAALERTL GVPVFQEQCM
QIAMIAADFT PGEADGLRRA MGAWRRTGGL GAYEHRLIDG MTRNDYPLEF AQRVVEQVKG
FSSYGFPESH AVSFALLVYD SCWIKRHHPA EFLAAMLNSQ PLGFYTPSQL VQDAQRHGVV
VRPVDVGHSD WDCTLEDMPA PEAPGHPVVR LGLRLVSGLS KEEALRISAA RAERPFTSTE
DLALRAQLDR QTLQQLAAAD ALQSLAGHRR QQVWDASALQ AAPAILRRAP VNEATLALDE
APEGEAIVWD YASTSLTLRR HPLALLRERL QKRRFMTAEQ LKAAPDGRLV RACGIVTCRQ
QPGTSQGVVF VTLEDETGTV QVIVWRALRE RQRAELTGSR LLAVHGVWQR EGEVCNLIAG
RLENLNPLLG RLAPGSRDFH
//
