ID A0A1V4HVX9_NITVU Unreviewed; 3250 AA.
AC A0A1V4HVX9;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:OPH82039.1};
GN ORFNames=B2M20_14300 {ECO:0000313|EMBL:OPH82039.1};
OS Nitrobacter vulgaris.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Nitrobacter.
OX NCBI_TaxID=29421 {ECO:0000313|EMBL:OPH82039.1, ECO:0000313|Proteomes:UP000189940};
RN [1] {ECO:0000313|EMBL:OPH82039.1, ECO:0000313|Proteomes:UP000189940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ab1 {ECO:0000313|EMBL:OPH82039.1,
RC ECO:0000313|Proteomes:UP000189940};
RA Mellbye B.L., Davis E.W., Spieck E., Chang J.H., Bottomley P.J.,
RA Sayavedra-Soto L.A.;
RT "Genome sequence of the nitrite-oxidizing bacterium Nitrobacter vulgaris
RT strain Ab1.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OPH82039.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MWPQ01000051; OPH82039.1; -; Genomic_DNA.
DR STRING; 29421.B2M20_14300; -.
DR OrthoDB; 9803968at2; -.
DR Proteomes; UP000189940; Unassembled WGS sequence.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:InterPro.
DR CDD; cd05930; A_NRPS; 1.
DR CDD; cd17649; A_NRPS_PvdJ-like; 1.
DR CDD; cd19543; DCL_NRPS; 1.
DR CDD; cd19534; E_NRPS; 1.
DR CDD; cd05931; FAAL; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR Gene3D; 3.30.300.30; -; 3.
DR Gene3D; 3.40.50.980; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 3.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 3.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR040097; FAAL/FAAC.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR NCBIfam; TIGR01720; NRPS-para261; 1.
DR PANTHER; PTHR45398; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR PANTHER; PTHR45398:SF1; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR Pfam; PF00501; AMP-binding; 3.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 3.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 6.
DR PROSITE; PS00455; AMP_BINDING; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT DOMAIN 573..648
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1629..1703
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3157..3232
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 3250 AA; 355109 MW; 48CB52D4F8530276 CRC64;
MYNDNLVERL REHVARRPDK AALRFLEQDD LVEELTFAEL DVRIRSVAAR LQQLGGAGER
ALILLPSGIN YVVAFYACLY SGVVAVPAYP PEGSSERYAG RLSGILRDAA PRFILLESDL
RSLVETAFPE LTDVQVIAVD SVLTEPADEW RETRLAADAI AFLQYTSGST SQPKGVCVSH
ANLAANERAM ESATGGTLDD VFVSWLPLYH DMGLIGGLLS PLFTGFTCVL MSPRNFLERP
RRWLEAIDRH GGTISGGPDF AYALCTDRVS DETIDRLNLS RWKFAFSGSE FVRHTTLQRF
GDRFARAGFD RRALNACYGL AEATLLVTAS DITAETISYT LDTAKLAAGL VACADKGTDL
VACGRPAADH ATRIMRVDGS VAAGADEVGE IWVSGPSVTL GYWNNPDATR QVFVERDGFR
WLRTGDLGFI RDGALVVAGR LKDVLIVRGQ NVYPTDVEQA VEADVNSVRR GRVAAFAVEA
EGREGIGVAA EFSRAVLKSE NPEALADAIG QAVLRQAQEY PAVIVLLNPR AMPLTTSGKL
QRSACAARLA NDTLDSFMVF EKGRRRDSTT LTPPVTDTER AVSLIWCDAL GLRAVHCEDD
FFFLGGNSIT AGQVASAIRE RFGLELELRS FFDAPTLAAF ASHIDSLART GVRPPLPSIP
KAAAADRATL SHAQERLWFL WNMDPVGTAY TVASAIRLKG GLDHAALTGA IDQIVCRHEA
LRTTFVAIEG RATQVVHDSM GVDIRHEDLR AYPANARDAH AAEIRRSELA KPFDLANGPL
LRTVLLQLAD DAHELLLLSH HIIVDGWSLD VLLQELSSLY RGALGQKADL PPVPAVQYAD
FAIWQRNWLA EGEGDRQLRY WRTKLGDEHP VLALPHDRPR PVTQSHGGDT VGFAIDGVLA
ARLQKVAAEH RVSVFMLILA AYQALLYRYC GQSDLRIGVP IAGRQQAQLE RLIGCFVNTL
VLRAKIVDDA SFTSFLQQVK ETVIEALSHQ DLPFEMLVDG LRPERNGSHN PLFQAKFNYM
TASRGFDGVD GLTADIDIMD LAGSHFDLAL DVVDSLSGMK ATFNYATDLF DRPTIERFAA
QFCLMLRQIV DDAERRISDF VIDDAGRQVV ATQSATFEFN DVVSLHRAST LGKRAVVAVR
YGDETLTFAD LEQRSNRIAN MLIARGVTRE VAVALWMERT PSFVVALLGV LKAGAAYVPL
DPKWPLERIK RILDDGRIEI LLATTENLTN SHALDCVVID ANAEATNEEI SDGPPETVIH
PAQTAYVIYT SGSTGTPKGV AVSHGALANY VQALLQRLQP QSSASMAMVS TVAADLGHTV
LFGALASGTT LNLLSSEAVL DADAFARAME EGKVGVLKIV PSHLRGLLQA RRSADLLPCE
TLILGGEACD AALLDEVRRL RPHCRIINHY GPTEATVGAA IHECGSEHKN GSVPIGLPLA
NLRVHVLDNA LNEVPIGVAG ELYIGGAGVA RGYRGSAGLT AERFVPDPFG AAGDRLYRTG
DRVRCDQAGR LIFIGRNDDQ IKLRGYRIEP DEVGRAIKAL PGINDAVVIA RSIDAKVGRQ
ELVAYCVPGD GMTLEAEAIK RQLSAFVPDY MVPSHIVVLE RLPLTPNGKI DRKALPESAG
GVAASNYVAP AGDTETTIAA VWCEVIGRER VGRNDNFFEL GGDSILSLQI VARLRKRGIR
LTPKQIFSQQ TIAGLAAVAI LTTASAPEKE RSGSRTGSDP APCMSHLLPI QTRFFTEEIE
NRNHWNQAVL LIPQGRMDWE MLRRALITVV EHHDALRLRF KQVDGAWRAQ KDVSPTPSDL
LWTHAGVGDA IQVTALASAA QRSLSLSSGP LLRAVGMDLA DGSQRLLLAI HHLVVDGVSW
RILLEDLASA YEQMEKGGAL ELAPKSDSYA SWGERLHTYA ASAEIASELP VWLDCEANSN
LPCDDNHGGV DLVGDSAEVS LVLDPELTSR LLEEAPAAYR TQVNDLMLAG LARAVSRWSG
CDDVTVELEG HGREDIFPGA DISRTVGWFT TAFPVRLQGG DDDASLIKAV KEKLRAIPNH
GLGYGVLRYL GSEEQRRALK RMAEPQIVFN YLGRFDASVG ESSRFLFAPE IAGSSRCASG
PMRGWLVING LVRDGRLRLS LEYGRKRFQR ATIERLAELY ETALRELVTH CCSGAFGLTP
SDVALSGLRQ SDLDRFALDA RNIQDIYPLS PMQQGMLFHA LRDGGNDAYV NQIGIELLGF
DAGKLRMAWQ AVSDRHAALR TGFVWQDLSG ATQQVVYRHV AVPFVEEDWR QRAAVLERDD
KRAELETALA EISRREYEKG FDLAQVPLQR VRLIHLDEKR HWLIWTHHHI LADGWSSARL
VAEVLQHVRE GVLPTAQGSY RDYIAWLQNR DSASAEKFWR DTLAELETPT LLANTMVTNK
ASEARGHASI VLAVNAELTW SLKSLAKRER VTLNTVIQAA WAQLLRRLSG QFAVCFGVTV
SGRPEELHGS EETVGLFINT LPIVDAPSPQ TKVGDWLRRL QEQNLVLREH GWTPLYEIQR
FAGHGGQTLF DNILVFENYP IDEGLQQAGG SGPRLGRVDH VTPTNFALAV AVFAGNDRLN
LEFNYDLARF DEASIQRLRD MLRGLLEQIA FDAGSPLGRL GSAVSDEARV FNWSNAAQLA
SPASSIGVVA HIEAQAARAR SSVAVIWRDR RLSYGELNTR ANQLARQLRG QGIGPDCLVG
IALARSPEMM VALLAVLKAG AAYLPLDPDY PAERLVHMLR DSGTRLILTE SEQLKSLSSV
LDQIAVETFC IDRPSLFAGE DAGNLNIEIH PDSLAYVIYT SGSTGTPKGV AVAHGPLAMH
CEAISRLYRM TSRDREFQTA SINFDIAHER WLVPLMSGGS LVLPSRPGLL VDDLVHEIER
SSVTTIFLPP AYADQLSAAL RQSGRKLSIR ACIVGGEAWL DTGIKAVREV IDTDLLINAY
GPTETVIAPT AWVVDDAKLK PRQAAPIGRP VGTRTAYILD ADLNVVPAGV TGELFIGGAG
LARGYLNRSA MTAERFIPDP FCANGDRLYR TGDLARWRDD GVIDYVGRAD LQVKIRGFRI
ELGEIEARLI EQRGVRAAAV VARESKTGRH LVAYVSGDAA LDGAALRDAL AAVLPDYMVP
ATIVVLEKLP LTLNGKIDRQ ALPAPHEDML ACRRYEAPED DVEIALARIW SEILGVERVG
RNDHFFELGG HSLLAVRVLS LVSQEIGVSI PISDLFAHPD LASFARVVSI SLIEQEFDAE
ELRELIAAEQ
//
