ID A0A1V4I991_9FIRM Unreviewed; 513 AA.
AC A0A1V4I991;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Citrate lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE Short=Citrase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE EC=2.8.3.10 {ECO:0000256|PIRNR:PIRNR009451};
DE EC=4.1.3.6 {ECO:0000256|PIRNR:PIRNR009451};
DE AltName: Full=Citrate (pro-3S)-lyase alpha chain {ECO:0000256|PIRNR:PIRNR009451};
DE AltName: Full=Citrate CoA-transferase subunit {ECO:0000256|PIRNR:PIRNR009451};
GN Name=citF {ECO:0000313|EMBL:OPJ56454.1};
GN ORFNames=CLOTH_08590 {ECO:0000313|EMBL:OPJ56454.1};
OS [Clostridium] thermoalcaliphilum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptostreptococcaceae.
OX NCBI_TaxID=29349 {ECO:0000313|EMBL:OPJ56454.1, ECO:0000313|Proteomes:UP000190140};
RN [1] {ECO:0000313|EMBL:OPJ56454.1, ECO:0000313|Proteomes:UP000190140}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 7309 {ECO:0000313|EMBL:OPJ56454.1,
RC ECO:0000313|Proteomes:UP000190140};
RA Poehlein A., Daniel R.;
RT "Genome sequence of Clostridium thermoalcaliphilum DSM 7309.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + citrate = (3S)-citryl-CoA + acetate;
CC Xref=Rhea:RHEA:19405, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089,
CC ChEBI:CHEBI:57288, ChEBI:CHEBI:57321; EC=2.8.3.10;
CC Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = acetate + oxaloacetate; Xref=Rhea:RHEA:10760,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:30089; EC=4.1.3.6;
CC Evidence={ECO:0000256|PIRNR:PIRNR009451};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR009451}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OPJ56454.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MZGW01000002; OPJ56454.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V4I991; -.
DR STRING; 29349.CLOTH_08590; -.
DR OrthoDB; 9767643at2; -.
DR Proteomes; UP000190140; Unassembled WGS sequence.
DR GO; GO:0009346; C:ATP-independent citrate lyase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008815; F:citrate (pro-3S)-lyase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008814; F:citrate CoA-transferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1080.10; Glutaconate Coenzyme A-transferase; 2.
DR InterPro; IPR006472; Citrate_lyase_asu.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR NCBIfam; TIGR01584; citF; 1.
DR PANTHER; PTHR40596; CITRATE LYASE ALPHA CHAIN; 1.
DR PANTHER; PTHR40596:SF1; CITRATE LYASE ALPHA CHAIN; 1.
DR Pfam; PF04223; CitF; 1.
DR PIRSF; PIRSF009451; Citrt_lyas_alpha; 1.
DR SUPFAM; SSF100950; NagB/RpiA/CoA transferase-like; 2.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|PIRNR:PIRNR009451};
KW Lyase {ECO:0000256|PIRNR:PIRNR009451, ECO:0000313|EMBL:OPJ56454.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000190140};
KW Transferase {ECO:0000256|PIRNR:PIRNR009451, ECO:0000313|EMBL:OPJ56454.1}.
SQ SEQUENCE 513 AA; 54935 MW; CF1A9623F11AC9AA CRC64;
MATNKIGREI PDYIEGIGEL RAYKGPFSIK PTTRKFGRKI STITPKESKM LESIEEAIIE
TGLKDGMTIS FHHHFREGDY IINMVVDAIA KLGIKNITLA PSSLNSVHAP IIEHIENGVI
TKIETSGLRG SLADAISKGI LEYPVKIRSH GGRARAIEAG EVKIDVAFLG VPACDEYGNA
NGHSGKSFCG SLGYAKVDAQ YADKVVLITD NLVPYPNVPA SISQTDVDFV VKIDAIGNPK
GIVSGATRYT KNPRELLIAK YAATVIKESG YLVDGFSMQC GTGGASLAVA RSIKQTMIEK
NIKASFALGG ITGQFVEMLE EGLIGKLYDT QCFDLTAAKS IGTNPNHVEI DAGFYANPHN
KGCAVNMLDI VILSALEIDT DFNVNVITGS DGVLRGASGG HCDTAAGSKL SIIVAPLIRG
RIPTIVESVN TVTTPGETID VVVTDRGIAI NPLRTDLIEK LSNTNLPLLT IEELKEKAEK
IAGKPKAIEY GEKIVALVEY RDGTIIDVIR EVK
//
