ID A0A1V4IW61_9CLOT Unreviewed; 710 AA.
AC A0A1V4IW61;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN Name=pulA {ECO:0000313|EMBL:OPJ64186.1};
GN ORFNames=CLORY_07510 {ECO:0000313|EMBL:OPJ64186.1};
OS Clostridium oryzae.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1450648 {ECO:0000313|EMBL:OPJ64186.1, ECO:0000313|Proteomes:UP000190080};
RN [1] {ECO:0000313|EMBL:OPJ64186.1, ECO:0000313|Proteomes:UP000190080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 28571 {ECO:0000313|EMBL:OPJ64186.1,
RC ECO:0000313|Proteomes:UP000190080};
RA Poehlein A., Daniel R.;
RT "Genome sequence of Clostridium oryzae DSM 28571.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OPJ64186.1}.
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DR EMBL; MZGV01000005; OPJ64186.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V4IW61; -.
DR SMR; A0A1V4IW61; -.
DR STRING; 1450648.CLORY_07510; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000190080; Unassembled WGS sequence.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR049117; pulA_all-beta.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF21653; pulA_all-beta; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Glycosidase {ECO:0000313|EMBL:OPJ64186.1};
KW Hydrolase {ECO:0000313|EMBL:OPJ64186.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000190080}.
FT DOMAIN 240..610
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 710 AA; 80432 MW; 68133C1977AC7F8D CRC64;
MQLLNCSLID FTTVCIKTDS SDYLNQQNIN VINGKKFISI EKTSIKDSCV YLKLTAEINI
KYKTIIEYDN QKFNVNYYSL YSTKEFDDRY YTEKPLGIHY SKTQTIFKLW SPIAENVDLL
IYDSANSVPS KFSMEETKNG VWQIKLSGDY NGFFYNYQVK IQGEENEIID PYARACSING
IKGAILDFSL SNPKGFSKDS HIPLSNYSDA VLYEINVRDI SIHPDSGIEK KGKFLGLCEE
NTSSSKNILT GLSHIKELGI THVQLMPVFD FSYQSVDEEH PYKYNWGYDP QNYNIPEGNY
STAPSNPLSR ILELKQAVAA FHRNNIGVIM DVVYNHIFKY EYSNLHNLFP HYFFRECENG
DISNGSGCGN DIASEHSMVR KFITDSLIYW MEEYHIDGFR FDLMGILDVD TVNYIISQLK
SRNSDVIIYG EGWNMGTAIA ECKRCSINNN RATKNLGFFN DVIRDSIKGS VFQENEKGFV
SGKQNLESMI KYCAAGCCIT VDDKSPIFDS PTQTINYISC HDNYTLWDKL KISCPGASEN
NLKAMCKLAY GILLTSQGIP LLHSGCEFCR TKSGISNSYN ATDIINRIDW NLKYKNMDLF
QYVKGLISIR RGHNAFREVS SEDIKDHLVF LPSPPNTVAY LLKDNGARDS WNDILIIYNS
TSETQSISIP DGSWNIVANG KSAGLEVLEK IEGSMITVSP VSAIIAYKSN
//
