ID A0A1V4J1T0_9CLOT Unreviewed; 469 AA.
AC A0A1V4J1T0;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=PTS system mannitol-specific EIICB component {ECO:0000256|ARBA:ARBA00021825};
DE EC=2.7.1.197 {ECO:0000256|ARBA:ARBA00011909};
DE AltName: Full=EIICB-Mtl {ECO:0000256|ARBA:ARBA00033349};
GN Name=mtlA {ECO:0000313|EMBL:OPJ66242.1};
GN ORFNames=CLCHR_01150 {ECO:0000313|EMBL:OPJ66242.1}, D2A34_21210
GN {ECO:0000313|EMBL:RII32741.1}, GKZ28_03485
GN {ECO:0000313|EMBL:MVX62762.1};
OS Clostridium chromiireducens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=225345 {ECO:0000313|EMBL:OPJ66242.1, ECO:0000313|Proteomes:UP000191056};
RN [1] {ECO:0000313|EMBL:OPJ66242.1, ECO:0000313|Proteomes:UP000191056}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 23318 {ECO:0000313|EMBL:OPJ66242.1,
RC ECO:0000313|Proteomes:UP000191056};
RA Poehlein A., Daniel R.;
RT "Genome sequence of Clostridium chromiireducens DSM 23318.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RII32741.1, ECO:0000313|Proteomes:UP000265930}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C1 {ECO:0000313|EMBL:RII32741.1,
RC ECO:0000313|Proteomes:UP000265930};
RA Xing M., Wei Y., Ang E.L., Zhao H., Zhang Y.;
RT "Genome of Clostridium chromiireducens C1, DSM12136.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:MVX62762.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NM79_F5 {ECO:0000313|EMBL:MVX62762.1};
RA Navarre W., Wong E.;
RT "Microbes associate with the intestines of laboratory mice.";
RL Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (sugar PTS), a major carbohydrate active transport system,
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane. The
CC enzyme II CmtAB PTS system is involved in D-mannitol transport.
CC {ECO:0000256|ARBA:ARBA00002434}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-mannitol(out) + N(pros)-phospho-L-histidyl-[protein] = D-
CC mannitol 1-phosphate(in) + L-histidyl-[protein];
CC Xref=Rhea:RHEA:33363, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:16899, ChEBI:CHEBI:29979, ChEBI:CHEBI:61381,
CC ChEBI:CHEBI:64837; EC=2.7.1.197;
CC Evidence={ECO:0000256|ARBA:ARBA00001655};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OPJ66242.1}.
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DR EMBL; WSRQ01000004; MVX62762.1; -; Genomic_DNA.
DR EMBL; MZGT01000001; OPJ66242.1; -; Genomic_DNA.
DR EMBL; QXDJ01000006; RII32741.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V4J1T0; -.
DR STRING; 225345.CLCHR_01150; -.
DR OrthoDB; 9814222at2; -.
DR Proteomes; UP000191056; Unassembled WGS sequence.
DR Proteomes; UP000265930; Unassembled WGS sequence.
DR Proteomes; UP000656077; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0022872; F:protein-N(PI)-phosphohistidine-mannitol phosphotransferase system transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR CDD; cd05567; PTS_IIB_mannitol; 1.
DR Gene3D; 3.40.50.2300; -; 1.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR029503; PTS_EIIB_mannitol.
DR InterPro; IPR003352; PTS_EIIC.
DR InterPro; IPR013014; PTS_EIIC_2.
DR InterPro; IPR004718; PTS_IIC_mtl.
DR NCBIfam; TIGR00851; mtlA; 1.
DR Pfam; PF02378; PTS_EIIC; 1.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF52794; PTS system IIB component-like; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
DR PROSITE; PS51104; PTS_EIIC_TYPE_2; 1.
PE 4: Predicted;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Reference proteome {ECO:0000313|Proteomes:UP000191056};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 21..44
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 50..69
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 81..112
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 246..265
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 272..294
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 314..337
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 15..344
FT /note="PTS EIIC type-2"
FT /evidence="ECO:0000259|PROSITE:PS51104"
FT DOMAIN 381..469
FT /note="PTS EIIB type-2"
FT /evidence="ECO:0000259|PROSITE:PS51099"
SQ SEQUENCE 469 AA; 48995 MW; B2EC390374C50DBB CRC64;
METNKTSLKG SVQRLGSFLS GMVLPNIGAF IAWGLITALF IPTGWVPNEY FGKLVGPMIT
YLLPILIGYT GGKLVYGQRG AVVGAIATAG VVVGASIPMF LGAMIMGPFG GFVIKKVDQL
FEGKVPTGFE MLVNNFSAGI FGAIVSLIGY TCIEPVVTAF SNTLGALATI VTNMGLLPLI
DIFIEPAKVL FLNNAINHGI LSPLGIQQAQ EVGKSIFFLL EANPGPGLGI LLAYTLYGKG
NAKQSAPGAI IIHFLGGIHE IYFPYILMKP VLIVAAIAGG ICADLVFVLT NAGLAAPASP
GSIFAVMAMT PKGGYFSVLA GVAVGAIVSF LVGSVILKAS KDDENEGNID EAQAKMKGMK
AESKGSKAAE TTVKVSKSDV KLIVFACDAG MGSSAMGESI LKKALKDAGI NDVAVKHSSV
DSIPQDADVV FTQENLVERA KKSANTANII TVKNFLDRSK YDEFISTLK
//
