ID A0A1V4J293_9CLOT Unreviewed; 1192 AA.
AC A0A1V4J293;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=dnaE {ECO:0000313|EMBL:OPJ65817.1};
GN ORFNames=CLCHR_03900 {ECO:0000313|EMBL:OPJ65817.1}, D2A34_04925
GN {ECO:0000313|EMBL:RII36729.1};
OS Clostridium chromiireducens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=225345 {ECO:0000313|EMBL:OPJ65817.1, ECO:0000313|Proteomes:UP000191056};
RN [1] {ECO:0000313|EMBL:OPJ65817.1, ECO:0000313|Proteomes:UP000191056}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 23318 {ECO:0000313|EMBL:OPJ65817.1,
RC ECO:0000313|Proteomes:UP000191056};
RA Poehlein A., Daniel R.;
RT "Genome sequence of Clostridium chromiireducens DSM 23318.";
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:RII36729.1, ECO:0000313|Proteomes:UP000265930}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C1 {ECO:0000313|EMBL:RII36729.1,
RC ECO:0000313|Proteomes:UP000265930};
RA Xing M., Wei Y., Ang E.L., Zhao H., Zhang Y.;
RT "Genome of Clostridium chromiireducens C1, DSM12136.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA polymerase III is a complex, multichain enzyme
CC responsible for most of the replicative synthesis in bacteria. This DNA
CC polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain
CC is the DNA polymerase. {ECO:0000256|ARBA:ARBA00025611}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: DNA polymerase III contains a core (composed of alpha, epsilon
CC and theta chains) that associates with a tau subunit. This core
CC dimerizes to form the POLIII' complex. PolIII' associates with the
CC gamma complex (composed of gamma, delta, delta', psi and chi chains)
CC and with the beta chain to form the complete DNA polymerase III
CC complex. {ECO:0000256|ARBA:ARBA00026073}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE
CC subfamily. {ECO:0000256|ARBA:ARBA00009496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OPJ65817.1}.
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DR EMBL; MZGT01000004; OPJ65817.1; -; Genomic_DNA.
DR EMBL; QXDJ01000001; RII36729.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1V4J293; -.
DR STRING; 225345.CLCHR_03900; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000191056; Unassembled WGS sequence.
DR Proteomes; UP000265930; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:OPJ65817.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000191056};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OPJ65817.1}.
FT DOMAIN 8..75
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1192 AA; 135775 MW; 685543BE9B6161DF CRC64;
MMEEKQFCHL HLHTEYSLLD GSGKVGKLMK RAKELGMKSI AITDHGVLYG LVDFFKAAKD
NDIKPILGCE VYVVPKSRHI KQPDKENSTY HLVLLVKDKV GYENLMKIVS VASIEGFYYK
PRVDYDYLRK HSEGLIALSA CLGGEVQSCH LKGNYEKAKE TALIYKEIFN GDFYLEIQNH
GMEEQKKVNE ENIKLSKETG IPLVATNDVH YISKEDSKSH DILMCIQTAK TIDDPHRRRY
PSDQFYLKSA EEMWDMFSYV PEAIENTIKI TEQCNYEYKF HESKLPKFPL EEGQDPFEYL
RDTCYKGLIE RYDVFEQLRD SDLDYNKINE IILKREEAKE YIDRLEYELE VIGQMGYVDY
FLIVWDFIRF SYESGIPTGP GRGSAAGSIV AYTLGITKID PIKYSLIFER FLNPERVSMP
DIDSDFCYER RQEVIDYVVD KYGADNVSQI ITFGTMAARL CIRDVGRAMN YSYAEVDRIA
KMIPTMLGIT IEKALDLNPE LKLAYDSDER VKALIDVSKD LEGLPRHSST HAAGVVIASR
PLVEYVPLQK NDEMIVTQFG MNTLEELGLL KMDFLGLRTL TVMNDAVKMI KENRGIDVDL
DKIDFEDEEV YKMIGDGNTA GVFQLESPGM TSFMKELKPD SFEDIIAGIS LYRPGPMAEI
PRYIEGKRNP EKATYLTKEL EPILKVTYGC LVYQEQVMQA VRDLAGYSMG RSDMVRRAMS
KKKHKVMEEE RKNFIHGIVE NGEVVVPGCV RNGISEEVAN KIFDSMMDFA SYAFNKSHAA
AYAVVGYQTA YLMKYYPVEM IAAMLNSIMG ISEKVAYYIG IAEDLGIQVL PPNINESFSR
FTVKGNKIRF GLAAIKNVGA NVVDSIAKAR EEKGKFESLV DFINKMDPSS INKRAVECLI
KAGALDDFKV FRSKMLAVHE KLIDNISSDK KRNIDGQISL FASEELKNPE VNYPNIKEFN
KRNLLAMEKE MTGLYITGHP LDDYAQSLKM QTTNEISKIF LVQETLDDAL ESDIGEVNIF
NRQDTLQDND RVILGGILAS VNQKVTRNNS IMAFLKLEDL TGTIEVIVFP KTLDKVKELC
VTDSLVIVKG RLSLKEDEPP KLICESIEPL EKINSSKVYL RVEDTIAAKE LNKKLKELLV
KEYIGDTPIY IFANKEKQKF RVPRDRWISL ESDAISLLKD NLGEENIKIQ DA
//
