ID A0A1V6LXD0_9BACT Unreviewed; 740 AA.
AC A0A1V6LXD0;
DT 07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT 07-JUN-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Transcription elongation factor GreA {ECO:0000256|ARBA:ARBA00013729, ECO:0000256|HAMAP-Rule:MF_00105};
DE AltName: Full=Transcript cleavage factor GreA {ECO:0000256|HAMAP-Rule:MF_00105};
GN Name=greA {ECO:0000256|HAMAP-Rule:MF_00105};
GN ORFNames=BIY37_11860 {ECO:0000313|EMBL:OQD44798.1};
OS Candidatus Brocadia sapporoensis.
OC Bacteria; Planctomycetota; Candidatus Brocadiia; Candidatus Brocadiales;
OC Candidatus Brocadiaceae; Brocadia.
OX NCBI_TaxID=392547 {ECO:0000313|EMBL:OQD44798.1, ECO:0000313|Proteomes:UP000242219};
RN [1] {ECO:0000313|EMBL:OQD44798.1, ECO:0000313|Proteomes:UP000242219}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=40 {ECO:0000313|EMBL:OQD44798.1,
RC ECO:0000313|Proteomes:UP000242219};
RX PubMed=27932661;
RA Ali M., Haroon M.F., Narita Y., Zhang L., Rangel Shaw D., Okabe S.,
RA Saikaly P.E.;
RT "Draft Genome Sequence of the Anaerobic Ammonium-Oxidizing Bacterium
RT 'Candidatus Brocadia sp. 40'.";
RL Genome Announc. 4:e01377-16(2016).
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreA releases sequences of 2 to 3 nucleotides.
CC {ECO:0000256|ARBA:ARBA00024916, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family.
CC {ECO:0000256|ARBA:ARBA00008213, ECO:0000256|HAMAP-Rule:MF_00105,
CC ECO:0000256|RuleBase:RU000556}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OQD44798.1}.
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DR EMBL; MJUW02000116; OQD44798.1; -; Genomic_DNA.
DR RefSeq; WP_070068044.1; NZ_MJUW02000116.1.
DR AlphaFoldDB; A0A1V6LXD0; -.
DR Proteomes; UP000242219; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.50.30; Transcription elongation factor, GreA/GreB, C-terminal domain; 1.
DR Gene3D; 1.10.287.180; Transcription elongation factor, GreA/GreB, N-terminal domain; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR006359; Tscrpt_elong_fac_GreA.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR NCBIfam; TIGR01462; greA; 1.
DR PANTHER; PTHR30437; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR PANTHER; PTHR30437:SF4; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR SUPFAM; SSF54534; FKBP-like; 1.
DR SUPFAM; SSF46557; GreA transcript cleavage protein, N-terminal domain; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00105}; Reference proteome {ECO:0000313|Proteomes:UP000242219};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00105};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00105}.
FT DOMAIN 586..656
FT /note="Transcription elongation factor GreA/GreB N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF03449"
FT DOMAIN 665..737
FT /note="Transcription elongation factor GreA/GreB C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01272"
SQ SEQUENCE 740 AA; 85882 MW; 961E9F8D054024A2 CRC64;
MSNEVLIHKN TFSINELISL VNTEQYNKLE EAWLGAIESN NNNLSALFEI VDLLMKREEK
KRAHDFLMTL TSLYKQKGLH QDVLEILKKI LEYNPKEKGL AKEFAECYSY IYKDRPYAKD
LITRTCPETT TDIRNAMKLL ERYFYLDQGN YVFHKSWGVG EVISVDTHSE KIHINFEKKS
NHSVAIDIAP NILQKLDNDD LLVMIFARKD ALQKMIEEQP VDLIKLTLKY FKGKASVSQL
KNRLIAGVIP PDGWGRWWTH TKKLLKKDPY IKLTEGTPTT SFLEFRTSPM THHQEILERL
NQTEDLDKKI EITKKYISEM RHTEVCKETL TQLSDEFKKA ADQCHDTNPS VALECLILLE
EMQCMLKAEP DKYKNTIETL IRKNNNLPAL VDAVGILEYK KYSLDLIKKI RQEKWQEDFA
HLLFINRGNL WEFIIKELIA ENKQHTIEEV AFKAFNHFNA YPEHYIWFCK NSMHGRYPEL
YKNIDPAIML NRLIELLDNL CFKIQKGRDG DFKTIVNKIK NLLEDKGTDY AINILNDANA
ESIYNVVSSS KGLEDWFKVS IESVIQDRYP ELFEKPGLPK LDEHKIYVTK EGYEKKKKEF
DHLMNVEFPE NARDLGEAIS RGDLRENAEY KAAREKQAML VEKAERMKSE LQRVVILDPH
SVHPDSASPG TRVTLRHQGK TELEIYTILG PWDVDIENGV ISYLSPIGKG LLNKNAGETV
TIKLPEGESS YEIIKIEKAL
//
