ID   A0A1V6TWV3_9EURO        Unreviewed;       762 AA.
AC   A0A1V6TWV3;
DT   07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2017, sequence version 1.
DT   13-SEP-2023, entry version 20.
DE   RecName: Full=Diphthine--ammonia ligase {ECO:0000256|ARBA:ARBA00018426};
DE            EC=6.3.1.14 {ECO:0000256|ARBA:ARBA00012089};
DE   AltName: Full=Diphthamide synthase {ECO:0000256|ARBA:ARBA00029814};
DE   AltName: Full=Diphthamide synthetase {ECO:0000256|ARBA:ARBA00031552};
GN   ORFNames=PENFLA_c003G00511 {ECO:0000313|EMBL:OQE30299.1};
OS   Penicillium flavigenum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=254877 {ECO:0000313|EMBL:OQE30299.1, ECO:0000313|Proteomes:UP000191342};
RN   [1] {ECO:0000313|Proteomes:UP000191342}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBT 14082 {ECO:0000313|Proteomes:UP000191342};
RX   PubMed=28368369; DOI=10.1038/nmicrobiol.2017.44;
RA   Nielsen J.C., Grijseels S., Prigent S., Ji B., Dainat J., Nielsen K.F.,
RA   Frisvad J.C., Workman M., Nielsen J.;
RT   "Global analysis of biosynthetic gene clusters reveals vast potential of
RT   secondary metabolite production in Penicillium species.";
RL   Nat. Microbiol. 2:17044-17044(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC         AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC         H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC         COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC         ChEBI:CHEBI:456215; EC=6.3.1.14;
CC         Evidence={ECO:0000256|ARBA:ARBA00001559};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OQE30299.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; MLQL01000003; OQE30299.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1V6TWV3; -.
DR   STRING; 254877.A0A1V6TWV3; -.
DR   OrthoDB; 103959at2759; -.
DR   Proteomes; UP000191342; Unassembled WGS sequence.
DR   GO; GO:0017178; F:diphthine-ammonia ligase activity; IEA:UniProtKB-EC.
DR   CDD; cd01994; Alpha_ANH_like_IV; 1.
DR   CDD; cd06155; eu_AANH_C_1; 1.
DR   CDD; cd06156; eu_AANH_C_2; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   Gene3D; 3.90.1490.10; putative n-type atp pyrophosphatase, domain 2; 1.
DR   Gene3D; 3.30.1330.40; RutC-like; 2.
DR   InterPro; IPR002761; Diphthami_syn_dom.
DR   InterPro; IPR030662; DPH6/MJ0570.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR035959; RutC-like_sf.
DR   InterPro; IPR006175; YjgF/YER057c/UK114.
DR   NCBIfam; TIGR00290; MJ0570_dom; 1.
DR   PANTHER; PTHR12196:SF2; DIPHTHINE--AMMONIA LIGASE; 1.
DR   PANTHER; PTHR12196; DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN; 1.
DR   Pfam; PF01902; Diphthami_syn_2; 1.
DR   Pfam; PF01042; Ribonuc_L-PSP; 1.
DR   SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR   SUPFAM; SSF55298; YjgF-like; 2.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000191342}.
FT   DOMAIN          12..283
FT                   /note="Diphthamide synthase"
FT                   /evidence="ECO:0000259|Pfam:PF01902"
SQ   SEQUENCE   762 AA;  83322 MW;  6601A26F5ECA0E57 CRC64;
     MSAAQPSAPG LNVIALISGG KDSLYSILHC IRNGHKVVAL ANLYPEPQNK TSTNPKHLRH
     DEEDDIDSFM YQTIGHNIIP LYETALQIPL YRQPITGGAV DTSRIYGTKP SEKDETESLV
     PLLQRIKKAH PEANAVSAGA ILSTYQRTRI ENVANRLGLV PLAWLWMYPS LPAPPARSAD
     TLAIRQAGLL EDMAAARCDA RIIKVASGGL DEGFLWENVS GAGSGGRMMR RYLVKAMSRF
     AAAEDIRGAV LGEGGEYETL ALDGPGFLWK QRIEVGSREE KVGEGGVAFV RLRGARCTAK
     SVWEIGDGIA PSDIRRPGLL DAVFEGVLGS ALDFSGSRDD SKSRAMTSTA RSPDWPVCEP
     VHRLSGSTWT ISNIVAPEAG PGAGEQMKAI AHKVQKVFKS FSHPKSGSRS TDDIVFATVL
     LNSMADFGSM NDIYVSLFKK PNPPARVTVA CGDRLPSNVK VMVTFVVDLG ARDRRQGLHV
     QSRSYWAPAN IGPYSQALSV PLQNASRIVY IAGQIPLDPG SMDLAQVEGP GSWAENYRLR
     AVLALQHLWR IGEAMQVNWW LGAVAFLARG EHANTQAQVA WRLWERMHAL PDNEDEDDDD
     GPQLDAWDIK YGRRTEELTD APVPSLPQFT ALVVSHTSIP PFLAVQVDEL PRGSDIEWQG
     LGGRCEQVKL DIKGGTSSTT MDNQYKYTNI EIDAGSSDET LKQILRSVNE AYRRDSSLSQ
     AVLYTTYPVP EPLWPVQVVP CRSVWGREGR RLSAGVVLQQ LS
//