ID   A0A1V8T7G4_9PEZI        Unreviewed;       472 AA.
AC   A0A1V8T7G4;
DT   07-JUN-2017, integrated into UniProtKB/TrEMBL.
DT   07-JUN-2017, sequence version 1.
DT   24-JAN-2024, entry version 20.
DE   RecName: Full=Citrate synthase {ECO:0000256|RuleBase:RU000441};
GN   ORFNames=B0A48_07794 {ECO:0000313|EMBL:OQO07224.1};
OS   Rachicladosporium antarcticum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Cladosporiales; Cladosporiaceae; Rachicladosporium.
OX   NCBI_TaxID=1507870 {ECO:0000313|EMBL:OQO07224.1, ECO:0000313|Proteomes:UP000192596};
RN   [1] {ECO:0000313|Proteomes:UP000192596}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCFEE 5527 {ECO:0000313|Proteomes:UP000192596};
RA   Coleine C., Masonjones S., Stajich J.E.;
RT   "Genomes of endolithic fungi from Antarctica.";
RL   Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + oxaloacetate + propanoyl-CoA = (2S,3S)-2-methylcitrate +
CC         CoA + H(+); Xref=Rhea:RHEA:23780, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57392, ChEBI:CHEBI:58853; EC=2.3.3.5;
CC         Evidence={ECO:0000256|ARBA:ARBA00036244};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16;
CC         Evidence={ECO:0000256|ARBA:ARBA00000308};
CC   -!- PATHWAY: Organic acid metabolism; propanoate degradation.
CC       {ECO:0000256|ARBA:ARBA00005026}.
CC   -!- SIMILARITY: Belongs to the citrate synthase family.
CC       {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|RuleBase:RU000441}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OQO07224.1}.
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DR   EMBL; NAJO01000015; OQO07224.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1V8T7G4; -.
DR   STRING; 1507870.A0A1V8T7G4; -.
DR   InParanoid; A0A1V8T7G4; -.
DR   OrthoDB; 3513214at2759; -.
DR   Proteomes; UP000192596; Unassembled WGS sequence.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   NCBIfam; TIGR01793; cit_synth_euk; 1.
DR   PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR11739:SF15; CITRATE SYNTHASE 3, MITOCHONDRIAL; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000192596};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000441}.
FT   ACT_SITE        308
FT                   /evidence="ECO:0000256|PIRSR:PIRSR610109-1"
FT   ACT_SITE        354
FT                   /evidence="ECO:0000256|PIRSR:PIRSR610109-1"
FT   ACT_SITE        411
FT                   /evidence="ECO:0000256|PIRSR:PIRSR610109-1"
SQ   SEQUENCE   472 AA;  51631 MW;  D756AB856D1F760D CRC64;
     MAISTRTATK ALRAAKLTNA TPLYHASRSY ATAEPDLKET LKSVIPAKRE LLKKVKAQSS
     KTIGEVKIEN TLGGMRGLKA MVWEGSVLDA NEGIRFHGRT IKDCQAELPK GTSGTEMLPE
     SMFWLLLTGQ VPSTNQVRVL SKELAQKAQI PAFVSQMLDN FPTDLHPMTQ FACAVSALNY
     ESKFAKAYEK GLNKADYWEP TFDDSISLLA KLPTIAAKIY QNAYRGGGAL PAEIDESQDW
     SYNFAAMLGK GGKENEGFQD LLRLYLALHG DHEGGNVSAH ATHLVGSALS DPFLSYSAGL
     QGLAGPLHGL AAQEVLRWIL QMQETVGTSF SDQDVKDYLW TTLKSGRVIP GYGHAVLRKP
     DPRFEALMDF AAAREDIAAN PVFQLVKKNS ELAPAVLTEH GKTKNPYPNV DSSSGVLFHH
     YGFKETLYYT ATFGVSRGLG PLAQLIWDRA LGLPIERPKS MNLQGLLDQA TK
//